Humoral and bronchial immune responses in cattle experimentally infected with Mycoplasma mycoides subsp. mycoides small colony type

Abdo, El-Mostafa; Nicolet, Jacques; Miserez, R.; Gonçalves, Raquel; Regalla, J.; Griot, Christian; Bensaid, Albert; Krampe, Marike; Frey, Joachim

doi:10.1016/s0378-1135(97)00184-3

Cited by 46 publications

(42 citation statements)

References 22 publications

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“…mycoides SC strains [1,9]. The group constituting African and Australian strains and the type strain PG1 was also characterised by the presence of a 70 kDa antigen, corresponding to lipoprotein LppB, which was not detected in European strains [9,29].…”

Section: Discussionmentioning

confidence: 98%

“…The library was plated according to the manufacturer's protocol using the E. coli host strain XL1-Blue MRF'. Screening of the library was performed following a standard protocol with serum from a cow experimentally infected with the Afadé strain (cow #512, serum taken at slaughter, 42 days post-infection) [1] used at a dilution of 1:100. Positive clones were selected, amplified and excised in vivo into pBK-CMV phagemids using the filamentous helper phage ExAssist (Stratagene) as indicated by the manufacturer.…”

Section: Strains Growth Conditions and Dna Extractionmentioning

confidence: 99%

“…DNA sequencing and analysis were performed as previously described by Abdo et al [1]. [12] and by the method of Kyte and Doolittle [14].…”

Section: Molecular Biology Techniques and Bioinformatic Analysismentioning

confidence: 99%

“…mycoides SC, the European strain L2 (cow #502) and African strain Afadé (cows #511 and #512), which have been described in detail [1]. Sera from cows that were naturally infected were obtained from F. Santini, Teramo, Italy and F. Poumarat, Lyon, France and had CFT titers ranging from 1:10 to 1:1280. approximately the same amount of protein in Freund incomplete adjuvant (Difco Laboratories) were performed 21 days and 33 days after the first injection.…”

Section: Cbpp Sera and Production Of Monospecific Rabbit Anti-lppcn'-mentioning

confidence: 99%

“…mycoides SC and reacted with serum from cows with CBPP, have molecular masses of 110, 98, 95 85, 80, 72, 62, 48, 44, and 39 kDa [1,9]. These typical antigens are currently used to confirm sera that were positive in the complement fixation test (CFT) for CBPP.…”

Section: Introductionmentioning

confidence: 99%

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Antigenic and genetic characterisation of lipoprotein lppC from Mycoplasma mycoides subsp. mycoides SC

et al. 2003

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-Lipoprotein LppC, an immunodominant antigen, and its corresponding gene lppC were characterised in Mycoplasma mycoides subspecies mycoides small colony (SC) type, the etiological agent of contagious bovine pleuropneumonia (CBPP). The lppC gene was found in the type strain of M. mycoides subsp. mycoides SC and in field strains isolated in Europe, Africa, and Australia, as well as in vaccine strains. Southern blot analysis indicated the presence of at least four copies of lppC in the genome of M. mycoides subsp. mycoides SC, of which only one seems to be functional. Genes homologous to lppC have also been detected in closely related mycoplasmas such as M. mycoides subsp. mycoides large colony (LC) type and in M. sp. bovine group 7. LppC is encoded as a precursor with a consensus sequence for a prokaryotic signal peptidase II. The amino acid sequence of LppC and its precursor showed similarity to both LppB (at the N-terminal domain) and LppQ (at the C-terminal domain), two lipoproteins described previously in M. mycoides subsp. mycoides SC. The N-terminal domain of the mature LppC seems to be surface exposed. The C-terminal domain presented an integral membrane structure made up of five repeated units, rich in hydrophobic and aromatic amino acids, which may have pore forming potential in the mycoplasmal membrane. A recombinant peptide representing the N-terminal half of LppC was obtained following cloning in vector pETHIS-1 and expression in Escherichia coli hosts. The recombinant protein was used on immunoblots for serological analysis of sera from cattle that were naturally or experimentally infected with M. mycoides subsp. mycoides SC.Mycoplasma mycoides subsp. mycoides SC / contagious bovine pleuropneumonia / multiple copy gene / antigenic protein / lipoprotein

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Section: Discussionmentioning

confidence: 98%

Section: Strains Growth Conditions and Dna Extractionmentioning

confidence: 99%

“…DNA sequencing and analysis were performed as previously described by Abdo et al [1]. [12] and by the method of Kyte and Doolittle [14].…”

Section: Molecular Biology Techniques and Bioinformatic Analysismentioning

confidence: 99%

Section: Cbpp Sera and Production Of Monospecific Rabbit Anti-lppcn'-mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antigenic and genetic characterisation of lipoprotein lppC from Mycoplasma mycoides subsp. mycoides SC

et al. 2003

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Contagious Bovine Pleuropneumonia

Thiaucourt¹,

Nwankpa²,

Amanfu³

2021

Veterinary Vaccines

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The protection rate of T1/44 and T1sr vaccines assessed in two independent vaccine trials varied between 33 and 67% (Thiaucourt et al., 2000). Animals were challenged against local pathogenic strains, 3 months after single vaccination. Further experiments demonstrated that T1/44 confers longer immunity than T1sr after a single dose, while a booster vaccination received 12 months after initial immunisation increased the efficacy of both vaccines (> 80%; Wesongaet al., 2000). In order to be effective vaccines must contain at least 10 7 CFU/dose of live Mmm (WOAH, 2021).Both vaccines confer low efficacy at primo-vaccination and short-term immunity, requiring re-vaccinations every 6 and 12 months respectively for T1sr and T1/44. T1/44 has residual virulence, which results in occasional cases of severe inflammation at the site of injection called Willems' reactions. These local reactions can evolve into systemic disease and may even result in death of the animals in the absence of antibiotic treatments, which can discourage vaccine acceptance by farmers . Willems' reactions are observed mainly upon first vaccination and with low occurrence after re-vaccination (Muuka et al., 2014). No side effects have been reported for T1sr.Both T1/44 and T1sr are currently produced as freeze-dried live vaccines with cold chain requirements until delivery. After reconstitution, delivery time is restricted to 30 min -2 hrs. In field conditions and with hot temperatures, viability rapidly drops below the minimum recommended titre affecting vaccine efficacy.The quality of CBPP vaccines is known to differ among producers / batches, which may result in variable vaccine efficacy. Due to the potential side effects and cold chain requirements, vaccine delivery should be restricted to fully trained veterinary personnel. GAPS :Vaccine safety: Side effects associated with the T1/44 strain are perceived as an important issue. For example, T1/44 is not accepted in some countries due to reported adverse reactions and T1sr is used instead, although the frequency of vaccination campaigns is normally once per year. Vaccine delivery can exacerbate side effects, since applying inappropriate administration routes (intradermal or intramuscular instead of subcutaneous) or using inappropriate needle gauge, etc. can result in increased adverse reactions and, thus, reduced acceptability by farmers.Vaccine efficacy: The true efficacy of T1 vaccines in the field is not well known. Control programs based on vaccination alone are not sufficient for CBPP eradication in the absence of additional countermeasures such as animal movement control. For ex.: Northern Namibia and Western Zambia, where CBPP mass vaccination campaigns are delivered annually, are still struggling to control the disease, with many outbreaks declared in 2021 and 2022.Vaccine delivery: Fully trained personnel is needed to manage vaccination, since administration problems such as use of wrong diluents and vial sizes, inappropriate number of doses per vial, etc. can result in reduced viability a...

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Domain analysis of lipoprotein LppQ in Mycoplasma mycoides subsp. mycoides SC

Bonvin-Klotz

Vilei

Kühni-Boghenbor

et al. 2007

Antonie van Leeuwenhoek

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The lipoprotein LppQ is the most prominent antigen of Mycoplasma mycoides subsp. mycoides small colony type (SC) during infection of cattle. This pathogen causes contagious bovine pleuropneumonia (CBPP), a devastating disease of considerable socio-economic importance in many countries worldwide. The dominant antigenicity and high specificity for M. mycoides subsp. mycoides SC of lipoprotein LppQ have been exploited for serological diagnosis and for epidemiological investigations of CBPP. Scanning electron microscopy and immunogold labelling were used to provide ultrastructural evidence that LppQ is located to the cell membrane at the outer surface of M. mycoides subsp. mycoides SC. The selectivity and specificity of this method were demonstrated through discriminating localization of extracellular (i.e., in the zone of contact with host cells) vs. integral membrane domains of LppQ. Thus, our findings support the suggestion that the accessible N-terminal domain of LppQ is surface exposed and such surface localization may be implicated in the pathogenesis of CBPP.

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Humoral and bronchial immune responses in cattle experimentally infected with Mycoplasma mycoides subsp. mycoides small colony type

Cited by 46 publications

References 22 publications

Antigenic and genetic characterisation of lipoprotein lppC from Mycoplasma mycoides subsp. mycoides SC

Antigenic and genetic characterisation of lipoprotein lppC from Mycoplasma mycoides subsp. mycoides SC

Contagious Bovine Pleuropneumonia

Domain analysis of lipoprotein LppQ in Mycoplasma mycoides subsp. mycoides SC

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