Humic acid effects on retrotransposon polymorphisms caused by zinc and iron in the maize (Zea mays L.) genome

Yiğider, Esma; Taspinar, Mahmut Sinan; Aydın, Murat; Ağar, Güleray

doi:10.1007/s42976-020-00111-3

Cited by 7 publications

(4 citation statements)

References 51 publications

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“…Evrensel et al ( 2011 ) investigated the mobility of Nikita and BARE-1 retrotransposons in barley ( Hordeum vulgare L.) under plant tissue culture conditions using the IRAP molecular marker technique and reported that the polymorphism that occurs in callus of different ages is due to the movements of Nikita and BARE-1 retrotransposons. Yigider et al ( 2020 ) determined the polymorphism resulting from the movement of some retrotransposons by heavy metal stress in maize using IRAP and REMAP techniques. In our previous study, where we determined the effect of deltamethrin and ALA applications on DNA methylation changes (Taspinar et al 2017 ), the high level of DNA methylation polymorphism caused by deltamethrin decreased to lower values with ALA.…”

Section: Resultsmentioning

confidence: 99%

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5-Aminolevulinic acid treatment mitigates pesticide stress in bean seedlings by regulating stress-related gene expression and retrotransposon movements

Yuksel,

Aydin,

Agar

et al. 2024

Protoplasma

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Overdoses of pesticides lead to a decrease in the yield and quality of plants, such as beans. The unconscious use of deltamethrin, one of the synthetic insecticides, increases the amount of reactive oxygen species (ROS) by causing oxidative stress in plants. In this case, plants tolerate stress by activating the antioxidant defense mechanism and many genes. 5-Aminolevulinic acid (ALA) improves tolerance to stress by acting exogenously in low doses. There are many gene families that are effective in the regulation of this mechanism. In addition, one of the response mechanisms at the molecular level against environmental stressors in plants is retrotransposon movement. In this study, the expression levels of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), and stress-associated protein (SAP) genes were determined by Q-PCR in deltamethrin (0.5 ppm) and various doses (20, 40, and 80 mg/l) of ALA-treated bean seedlings. In addition, one of the response mechanisms at the molecular level against environmental stressors in plants is retrotransposon movement. It was determined that deltamethrin increased the expression of SOD (1.8-fold), GPX (1.4-fold), CAT (2.7-fold), and SAP (2.5-fold) genes, while 20 and 40 mg/l ALA gradually increased the expression of these genes at levels close to control, but 80 mg/l ALA increased the expression of these genes almost to the same level as deltamethrin (2.1-fold, 1.4-fold, 2.6-fold, and 2.6-fold in SOD, GPX, CAT, and SAP genes, respectively). In addition, retrotransposon-microsatellite amplified polymorphism (REMAP) was performed to determine the polymorphism caused by retrotransposon movements. While deltamethrin treatment has caused a decrease in genomic template stability (GTS) (27%), ALA treatments have prevented this decline. At doses of 20, 40, and 80 mg/L of ALA treatments, the GTS ratios were determined to be 96.8%, 74.6%, and 58.7%, respectively. Collectively, these findings demonstrated that ALA has the utility of alleviating pesticide stress effects on beans.

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Section: Resultsmentioning

confidence: 99%

“…The REMAP reactions were based on a previously published method (Yigider et al 2020 ). For amplification, the IRAP primers [Nikita-E2647, Stowaway, Sukkula, and Bare 1(0)] were combined with ISSR primers (8081, 8082) (Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

5-Aminolevulinic acid treatment mitigates pesticide stress in bean seedlings by regulating stress-related gene expression and retrotransposon movements

Yuksel,

Aydin,

Agar

et al. 2024

Protoplasma

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“…The REMAP reactions were based on previously published method (Yigider et al 2020). For ampli cation the IRAP primers [Nikita-E2647, Stowaway, Sukkula and Bare 1(0)] were combined with ISSR primers (8081, 8082) (Table S2).…”

Section: Remapmentioning

confidence: 99%

Overexpression of Some Stress Tolerance Genes Confers Enhanced Pesticide Tolerance in 5-Aminolevulinic Acid Treated Bean Seedlings

Aydın¹,

Arslan²,

Ağar³

et al. 2021

Preprint

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Overdose of pesticides leads to a decrease in yield and quality of plants such as bean. The unconscious use of deltamethrin, one of the synthetic insecticides, increases the amount of reactive oxygen species (ROS) by causing oxidative stress in plants. In this case, plants tolerate to stress by activating the antioxidant defense mechanism and many genes. 5-Aminolevulinic acid (ALA) improves tolerance to stress by external administration in low doses. There are many gene families that are effective in the regulation of this mechanism. In addition, one of the response mechanisms at the molecular level against environmental stressors in plants is retrotransposon movements. In this study, the expression levels of SOD, GPX, CAT and SAP genes were determined by Q-PCR in deltamethrin (0.5 ppm) and various doses (20, 40 and 80 mg/l) of ALA treated bean seedlings. It was determined that while deltamethrin increased the expression of these genes, 20 and 40 mg/l ALA gradually increased the expression of genes at levels close to control, but 80 mg/l ALA increased almost the same level as deltamethrin. In addition, REMAP was performed to determine the polymorphism caused by retrotransposon movements. While deltamethrin treatment has caused to decrease of genomic template stability (GTS), ALA treatments have prevented this decline. Collectively, these findings demonstrated that ALA gives the utility for alleviating pesticide stress effects on bean.

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“…Heavy metal stress also induced the retrotransposon (Nikita-57 N (57), Sukkula, Stowaway, Nikita E2647, and WLTR2105) in maize. As a result of IRAP and REMAP analyses Zn (from 37.4 to 48.2%) and Fe (from 22.9 to 36.4%), for Zn from 74.3 to 58.0% and for Fe from 87.6 to 65.4% polymorphism ratios were observed via IRAP and REMAP analyses, respectively(Yigider et al 2021).…”

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confidence: 93%

Investigation the effect of drought stress on tolerant and resistant rice (Oryza sativa L.) varieties in terms of morphologic and genetic characters

Arvas

YOLCİ

Marakli

2022

Genet Resour Crop Evol

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Drought associated with global warming is a major limiting factor for crop production. This abiotic stress affects the quality and yield of rice ( Oryza sativa L.) which is a model organism and also one of the major consumed products all over the world. In this study, we aimed to analyse morphologic (root length, shoot length, root wet weight, shoot wet weight, root dry weight and shoot dry weight) of rice varieties (dhan 65, dhan 71, kızıltan and Karacadağ local rice) grown under drought stress during 21 days. For genetic properties, Hopi and Osr30 retrotransposon movements were investigated by using IRAP marker technique. Depending on the decreasing in the amount of water, we observed that all growth parameters showed variable results in four varieties. Moreover, IRAP-PCR indicated different polymorphism ratios among samples. These rates were 0-75% for Hopi and 0-50% for Osr30 . As far as we know, this is the rst detailed report on dhan 65, dhan 71, kızıltan and Karacadağ local rice varieties grown under drought stress.

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Humic acid effects on retrotransposon polymorphisms caused by zinc and iron in the maize (Zea mays L.) genome

Cited by 7 publications

References 51 publications

5-Aminolevulinic acid treatment mitigates pesticide stress in bean seedlings by regulating stress-related gene expression and retrotransposon movements

5-Aminolevulinic acid treatment mitigates pesticide stress in bean seedlings by regulating stress-related gene expression and retrotransposon movements

Overexpression of Some Stress Tolerance Genes Confers Enhanced Pesticide Tolerance in 5-Aminolevulinic Acid Treated Bean Seedlings

Investigation the effect of drought stress on tolerant and resistant rice (Oryza sativa L.) varieties in terms of morphologic and genetic characters

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