Humic acid-assisted synthesis of stable copper nanoparticles as a peroxidase mimetic and their application in glucose detection

Abstract

In this report, stable copper nanoparticles (Cu NPs) were prepared by a facile annealing process using humic acid as the reducing and stabilizing agent. The products were characterized by X-ray powder diffraction, scanning electron microscope and Fourier transform infrared spectroscopy. The prepared Cu NPs show remarkable intrinsic peroxidase-like activity, which can fleetly catalyze the oxidation of peroxidase substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce a blue 10 color reaction. The detection limit of H 2 O 2 by Cu NPs can be as low as 1.32 × 10 -7 M. More importantly, the prepared Cu NPs shows excellent stability, which can hardly been oxidized even after 6 months. Based on the above mechanism, a simple, rapid and selective colorimetric method for the glucose detection was developed, and the detection limit of the glucose was 6.86 × 10 -7 M. This work provides a novel method for the preparation of stable Cu NPs, which may have wide applications on the detection of 15 glucose in human body and pear juice.

Peroxidase‐Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors

“…Based on the peroxidase‐like activity of Cu NCs, the Cu NCs showed a linear range from 1.0 × 10 −4 mol L −1 to 2 × 10 −3 mol L −1 and a LOD of 1.0 × 10 −4 mol L −1 for the colorimetric biosensing of glucose. The peroxidase‐like activity of the Cu NPs and its application for colorimetric biosensing of glucose was reported Ai et al In this report, the authors prepared the stable Cu NPs through a facile annealing process using humic acid as the reductant and stabilizer. The as‐prepared Cu NPs indicate excellent stability which can use to oxidize even after 6 months.…”

“…Metal‐based nanomaterials including gold nanoparticles (nanoclusters) (Au NPs, Au NCs), silver nanoparticles (Ag NPs), platinum nanotubes (nanoclusters, nanoparticles) (Pt NTs, Pt NCs, Pt NPs), rhodium nanoparticles (Rh NPs), copper nanoparticles (nanoclusters) (Cu NPs, Cu NCs), bimetal nanomaterials, and iridium nanoparticles (Ir NPs), were shown to have great intrinsic peroxidase‐like activity and applied to various applications. The peroxidase‐like activity of Au NPs was reported by Li et al in 2010 for the first time .…”

New Organomineral Complex from Humic Substances Extracted from Poultry Wastes: Synthesis, Characterization and Controlled Release Study

“…However, the humification and aromaticity content are not only related to the qualitative composition of functional groups, but also to the structural reorganization of such compounds, for example, the modifications that occur when the humic fractions complex with metals. 27,28 We highlight that for the FA-Zn and FA-Cu complexes the assigned peaks are not so intense, which may have impaired the calculation of aromatic and aliphatic carbon.…”

Systematic study on preparation of copper nanoparticle embedded porous carbon by carbonization of metal–organic framework for enzymatic glucose sensor

“…Recently, metal nanoparticle 39 or metal nanoparticle embedded composite materials have been widely used for enzyme mimicking catalysis applications. 40 Among many biological catalytic reactions, peroxidase-like catalytic activity has drawn much attention due to its wide potential applications (such as decomposition of reactive oxygen species).…”