Humane Endpoints and End of Life in Primates Used in Laboratories

Wolfensohn, Sarah

doi:10.1007/978-3-030-82708-3_16

Cited by 2 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Severity (Supplementary Table 3) was determined by the extent and distribution of inflammation, vascular change (infarction, thrombosis, pregnancy-associated vascular remodeling and/or the lack thereof), and non-vascular necrosis across the tissue section or organ (multiple slides were necessary to evaluate the placenta). Scores were averaged and compared between treatment groups as previously reported [24].…”

Section: Histologymentioning

confidence: 99%

Listeria monocytogenes infection in pregnant macaques alters the maternal gut microbiome

Hugon,

Deblois,

Simmons

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

Objectives The bacterium Listeria monocytogenes (Lm) is associated with adverse pregnancy outcomes. Infection occurs through consumption of contaminated food that is disseminated to the maternal–fetal interface. The influence on the gastrointestinal microbiome during Lm infection remains unexplored in pregnancy. The objective of this study was to determine the impact of listeriosis on the gut microbiota of pregnant macaques. Methods A non-human primate model of listeriosis in pregnancy has been previously described. Both pregnant and non-pregnant cynomolgus macaques were inoculated with Lm and bacteremia and fecal shedding were monitored for 14 days. Non-pregnant animal tissues were collected at necropsy to determine bacterial burden, and fecal samples from both pregnant and non-pregnant animals were evaluated by 16S rRNA next-generation sequencing. Results Unlike pregnant macaques, non-pregnant macaques did not exhibit bacteremia, fecal shedding, or tissue colonization by Lm. Dispersion of Lm during pregnancy was associated with a significant decrease in alpha diversity of the host gut microbiome, compared to non-pregnant counterparts. The combined effects of pregnancy and listeriosis were associated with a significant loss in microbial richness, although there were increases in some genera and decreases in others. Conclusions Although pregnancy alone is not associated with gut microbiome disruption, we observed dysbiosis with listeriosis during pregnancy. The macaque model may provide an understanding of the roles that pregnancy and the gut microbiota play in the ability of Lm to establish intestinal infection and disseminate throughout the host, thereby contributing to adverse pregnancy outcomes and risk to the developing fetus.

show abstract

Section: Histologymentioning

confidence: 99%

Listeria monocytogenes infection in pregnant macaques alters the maternal gut microbiome

Hugon,

Deblois,

Simmons

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…The copyright holder for this preprint this version posted June 19, 2023. ; https://doi.org/10.1101/2023.06.18.545418 doi: bioRxiv preprint distribution of inflammation, vascular change (infarction, thrombosis, pregnancy-associated vascular remodeling and/or the lack thereof), and non-vascular necrosis across the tissue section or organ (multiple slides were necessary to evaluate the placenta). Scores were averaged and compared between treatment groups as previously reported [24].…”

Section: Histologymentioning

confidence: 99%

Listeria monocytogenesinfection in pregnant macaques alters the maternal gut microbiome

Hugon

Golos

2023

Preprint

View full text Add to dashboard Cite

Objectives The bacterium Listeria monocytogenes (Lm) is associated with adverse pregnancy outcomes. Infection occurs through consumption of contaminated food that is disseminated to the maternal-fetal interface. The influence on the gastrointestinal microbiome during Lm infection remains unexplored in pregnancy. The objective of this study was to determine the impact of listeriosis on the gut microbiota of pregnant macaques. Methods A nonhuman primate model of listeriosis in pregnancy has been previously described [1, 2]. Both pregnant and nonpregnant cynomolgus macaques were inoculated with L. monocytogenes and bacteremia and fecal shedding were monitored for 14 days. Nonpregnant animal tissues were collected at necropsy to determine bacterial burden, and fecal samples from both pregnant and nonpregnant animals were evaluated by 16S rRNA next-generation sequencing. Results Unlike pregnant macaques, nonpregnant macaques did not exhibit bacteremia, fecal shedding, or tissue colonization by Lm. Dispersion of Lm during pregnancy was associated with a significant decrease in alpha-diversity of the host gut microbiome, compared to nonpregnant counterparts. The combined effects of pregnancy and listeriosis were associated with a significant loss in microbial richness, although there were increases in some genera and decreases in other. Conclusions Although pregnancy alone is not associated with gut microbiome disruption, we observed dysbiosis with listeriosis during pregnancy. The macaque model may provide an understanding of the roles that pregnancy and the gut microbiota play in the ability of Lm to establish intestinal infection and disseminate throughout the host, thereby contributing to adverse pregnancy outcomes and risk to the developing fetus.

show abstract

Humane Endpoints and End of Life in Primates Used in Laboratories

Cited by 2 publications

References 31 publications

Listeria monocytogenes infection in pregnant macaques alters the maternal gut microbiome

Listeria monocytogenes infection in pregnant macaques alters the maternal gut microbiome

Listeria monocytogenesinfection in pregnant macaques alters the maternal gut microbiome

Contact Info

Product

Resources

About