Human Vγ9/Vδ2 effector memory T cells express the killer cell lectin-like receptor G1 (KLRG1)

Eberl, Matthias; Engel, Rosel; Aberle, Silke; Fisch, Paul; Jomaa, Hassan; Pircher, Hanspeter

doi:10.1189/jlb.0204096

Cited by 34 publications

(26 citation statements)

References 18 publications

(32 reference statements)

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“…Similar to NK cells, γδ T cells have little effect on murine LCMV infection and CTL-mediated clearance , Eberl et al 2005. γδ T cells have been implicated in immunosurveillance of tumours and infections via cytokine secretion and cytolysis (Poccia et al 2002, Jacobs et al 2005.…”

Section: Discussionmentioning

confidence: 99%

“…Since CD3 + CD16 + cells often include γδ T cells (Eberl et al 2005), we also examined that population using flow cytometry. Vγ9 T cells, which represent approximately 80% of the γδ T cells in primate peripheral blood, exhibited a similar pattern of reduction in the percentage and absolute numbers as the NK cells (CD3 -CD8 + ) after the second day of infection (Fig.…”

Section: Viremia Was Detected 4-6 Days After Inoculation With Lcmv-we -mentioning

confidence: 99%

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Circulating natural killer and γδ T cells decrease soon after infection of rhesus macaques with lymphocytic choriomeningitis virus

Rodas

Cairo

Djavani

et al. 2009

Mem. Inst. Oswaldo Cruz

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Rhesus macaques infected with the WE strain of lymphocytic choriomeningitis virus (LCMV-WE) serve as a model for human infection with Lassa fever virus. To identify the earliest events of acute infection, rhesus macaques were monitored immediately after lethal infection for changes in peripheral blood mononuclear cells (PBMCs). Changes in CD3, CD4, CD8 and CD20 subsets did not vary outside the normal fluctuations of these blood cell populations; however, natural killer (NK) and γδ T cells increased slightly on day 1 and then decreased significantly after two days. The NK subsets responsible for the decrease were primarily CD3 -CD8 + or CD3 -CD16 + and not the NKT (primarily CD3 + CD56 + ) subset. Macaques infected with a non-virulent arenavirus, LCMV-Armstrong, showed a similar drop in circulating NK and γδ T cells, indicating that this is not a pathogenic event. Vγ9 T cells, representing the majority of circulating γδ T cells in rhesus macaques, displayed significant apoptosis when incubated with LCMV in cell culture; however, the low amount of cell death for virus-co-cultured NK cells was insufficient to account for the observed disappearance of this subset. Our observations in primates are similar to those seen in LCMV-infected mice, where decreased circulating NK cells were attributed to margination and cell death. Thus, the disappearance of these cells during acute hemorrhagic fever in rhesus macaques may be a cytokine-induced lymphopenia common to many virus infections. Keywords: NK cells -γδ T -rhesus macaque -LCMV -hemorrhagic feverArenaviruses are rodent-borne pathogens that have occasionally been known to cause lethal diseases in human beings (Oldstone 2002, Salvato & Rodas 2005. Every year, Lassa fever and the South American hemorrhagic fever viruses account for almost half a million cases worldwide, with approximately 16% mortality (Jahrling et al. 1985, McCormick et al. 1986, Fisher-Hoch et al. 2000. Although rodents serve as a reservoir for arenaviruses, studies of hemorrhagic fever have relied on guinea pig, hamster and primate models rather than mouse models because the disease mechanism is fundamentally different in non-reservoir species (Peters et al. 1987).Numerous murine studies of viral persistence and cell-mediated immunity have involved the prototype arenavirus, lymphocytic choriomeningitis virus (LCMV) , Ahmed et al. 1984, Salvato et al. 1991. The WE strain of LCMV is hepatotropic in mice, guinea pigs and primates (Riviere et al. 1985, Zinkernagel et al. 1986, Lukashevich et al. 2002, but in contrast to murine LCMV infections, LCMV-WE infection of rhesus macaques can resemble Lassa hemorrhagic fever in human beings (Jahrling et al. 1980, Peters et al. 1987, Lukashevich et al. 2002. The Armstrong (ARM) strain of LCMV does not cause overt disease in monkeys, even after intravenous inoculation (Danes et al. 1963, Peters et al. 1987, Lukashevich et al. 2002.Several immunological studies have shown the importance of CD8 T lymphocytes in both protection and in induction of immuno-pathogenesis i...

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Section: Discussionmentioning

confidence: 99%

Section: Viremia Was Detected 4-6 Days After Inoculation With Lcmv-we -mentioning

confidence: 99%

Circulating natural killer and γδ T cells decrease soon after infection of rhesus macaques with lymphocytic choriomeningitis virus

Rodas

Cairo

Djavani

et al. 2009

Mem. Inst. Oswaldo Cruz

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“…Human KLRG1 is also found on a subset of γδ T cells (Eberl et al 2005) and in a large proportion of CD4 + and CD8 + T cells found in cord blood (Marcolino et al 2004). In young adults, the expression of KLRG1 is about 40% on CD8 + T cells and 20% on CD4 + T cells (Voehringer et al 2002).…”

Section: Klrg1-more Than a Marker For T Cell Memorymentioning

confidence: 99%

KLRG1—more than a marker for T cell senescence

Henson

Akbar

2009

AGE

144

118

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The co-inhibitory receptor killer-cell lectin like receptor G1 (KLRG1) is expressed on NK cells and antigen-experienced T cells and has been postulated to be a marker of senescence. Whilst KLRG1 has frequently been used as a marker of cellular differentiation, data are emerging indicating that KLRG1 plays an inhibitory role. In this review we examine evidence highlighting this view of KLRG1 with emphasis on the functional defects that arise during T cell differentiation with age that may, in part, be actively maintained by inhibitory receptor signalling.

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“…It is well known that gdT cells express several NK receptors [31] and that their activity is regulated by both positive and inhibitory signals transduced by cell surface receptors including the C-type lectin family, immunoglobulin (Ig) superfamily and natural cytotoxicity receptors [32][33][34]. To characterize the activation state as well as NK and chemokine receptor expression, PE-gdT from TB and PB-gdT from TB and HS were analysed by flow cytometry.…”

Section: Characterization Of Gdt Cells In Peripheral Blood and Pleuramentioning

confidence: 99%

CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy

Yokobori

Schierloh

Geffner

et al. 2009

Clinical and Experimental Immunology

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SummaryTuberculous pleurisy is a naturally occurring site of Mycobacterium tuberculosis (Mtb) infection. Herein, we describe the expression of activation, natural killer (NK) and cell migration markers, as well as effector functions from gdT cells in peripheral blood (PB) and pleural effusion (PE) from tuberculosis patients (TB). We observed a decreased percentage of circulating gdT from TB patients and differential expression of NK as well as of chemokine receptors on PB and PE. Two subsets of gdT cells were differentiated by the CD3/gdT cell receptor (gdTCR) complex. The gdTCR low subset had a higher CD3 to TCR ratio and was enriched in Vd2 + cells, whereas most Vd1 + cells belonged to the gdTCR high subset. In PB from TB, most gdTCR high were CD45RA + CCR7 -and gdTCR low were CD45RA +/-CCR7 + CXCR3 + . In the pleural space the proportion of CD45RA -CCR7 + CXCR3 + cells was higher. Neither spontaneous nor Mtbinduced interferon (IFN)-g production was observed in PB-gdT cells from TB; however, PE-gdT cells showed a strong response. Both PB-and PE-gd T cells expressed surface CD107a upon stimulation with Mtb. Notably, PE-gdTCR low cells were the most potent effector cells. Thus, gdT cells from PB would acquire a further activated phenotype within the site of Mtb infection and exert full effector functions. As gdT cells produce IFN-g within the pleural space, they would be expected to play a beneficial role in tuberculous pleurisy by helping to maintain a T helper type 1 profile.

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Human Vγ9/Vδ2 effector memory T cells express the killer cell lectin-like receptor G1 (KLRG1)

Cited by 34 publications

References 18 publications

Circulating natural killer and γδ T cells decrease soon after infection of rhesus macaques with lymphocytic choriomeningitis virus

Circulating natural killer and γδ T cells decrease soon after infection of rhesus macaques with lymphocytic choriomeningitis virus

KLRG1—more than a marker for T cell senescence

CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy

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