Human Vascular Wall Microfluidic Model for Preclinical Evaluation of Drug-Induced Vascular Injury

Ersland, Erik E; Ebrahim, Neven; Mwizerwa, Olive; Oba, Takahiro; Oku, Keisuke; Nishino, Masafumi; Hikimoto, Daichi; Miyoshi, Hayato; Tomotoshi, Kimihiko; Rahmanian, Omid; Ekwueme, Emmanuel C.; Neville, Craig M.; Sundback, Cathryn A.

doi:10.1089/ten.tec.2021.0227

Cited by 4 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A variant of MPFRAP has been used to validate the simulated interstitial flow and diffusion within a microfluidic device that controls cellular communication mediated by interstitial flow 24 . Shear is a salient feature of the flow profiles in narrow microfluidic channels and has been exploited in numerous such devices to stimulate biological responses in cells 25 , 26 . Due to their small size, the aqueous flow in microfluidic devices is typically laminar, and diffusion is a prominent process by which solutes can be separated 27 , 28 .…”

Section: Discussionmentioning

confidence: 99%

“… 24 Shear is a salient feature of the flow profiles in narrow microfluidic channels and has been exploited in numerous such devices to stimulate biological responses in cells. 25 , 26 Due to their small size, the aqueous flow in microfluidic devices is typically laminar, and diffusion is a prominent process by which solutes can be separated. 27 , 28 For example, a T-sensor is a diagnostic microfluidic device for chemical assays which utilizes the diffusion of solutes to extract information about the solute molecular weight and concentration.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow

Elias

Brown

2023

J. Biomed. Opt.

View full text Add to dashboard Cite

Multi-photon fluorescence recovery after photobleaching (MPFRAP) is a nonlinear microscopy technique used to measure the diffusion coefficient of fluorescently tagged molecules in solution. Previous MPFRAP fitting models calculate the diffusion coefficient in systems with diffusion or diffusion in laminar flow. Aim:We propose an MPFRAP fitting model that accounts for shear stress in laminar flow, making it a more applicable technique for in vitro and in vivo studies involving diffusion.Approach: Fluorescence recovery curves are generated using high-throughput molecular dynamics simulations and then fit to all three models (diffusion, diffusion and flow, and diffusion and shear flow) to define the limits within which accurate diffusion coefficients are produced. Diffusion is simulated as a random walk with a variable horizontal bias to account for shear flow.Results: Contour maps of the accuracy of the fitted diffusion coefficient as a function of scaled velocity and scaled shear rate show the parameter space within which each model produces accurate diffusion coefficients; the shear-flow model covers a larger area than the previous models. Conclusion:The shear-flow model allows MPFRAP to be a viable optical tool for studying more biophysical systems than previous models.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow

Elias

Brown

2023

J. Biomed. Opt.

View full text Add to dashboard Cite

show abstract

Investigation of the Dysfunction Caused by High Glucose, Advanced Glycation End Products, and Interleukin‐1 Beta and the Effects of Therapeutic Agents on the Microphysiological Artery Model

Nam,

Kim,

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Diabetes mellitus (DM) has substantial global implications and contributes to vascular inflammation and the onset of atherosclerotic cardiovascular diseases. However, translating the findings from animal models to humans has inherent limitations, necessitating a novel platform. Therefore, herein, an arterial model is established using a microphysiological system. This model successfully replicates the stratified characteristics of human arteries by integrating collagen, endothelial cells (ECs), and vascular smooth muscle cells (VSMCs). Perfusion via a peristaltic pump shows dynamic characteristics distinct from those of static culture models. High glucose, advanced glycation end products (AGEs), and interleukin‐1 beta are employed to stimulate diabetic conditions, resulting in notable cellular changes and different levels of cytokines and nitric oxide. Additionally, the interactions between the disease models and oxidized low‐density lipoproteins (LDL) are examined. Finally, the potential therapeutic effects of metformin, atorvastatin, and diphenyleneiodonium are investigated. Metformin and diphenyleneiodonium mitigate high‐glucose‐ and AGE‐associated pathological changes, whereas atorvastatin affects only the morphology of ECs. Altogether, the arterial model represents a pivotal advancement, offering a robust and insightful platform for investigating cardiovascular diseases and their corresponding drug development.

show abstract

Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling

Nam,

Lee,

Ahmad

et al. 2024

BioChip J

View full text Add to dashboard Cite

The vascular system, essential for human physiology, is vital for transporting nutrients, oxygen, and waste. Since vascular structures are involved in various disease pathogeneses and exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific vascular models. While animal models possess sophisticated vascular morphologies, they exhibit significant discrepancies from human tissues due to species differences, which limits their applicability. To overcome the limitations arising from these discrepancies and the oversimplification of 2D dish cultures, microphysiological systems (MPS) have emerged as a promising alternative. These systems more accurately mimic the human microenvironment by incorporating cell interactions, physical stimuli, and extracellular matrix components, thus facilitating enhanced tissue differentiation and functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model and patient responses. This review focuses on recent advancements in MPS, particularly in modeling the human organ-specific vascular system, and discusses their potential in biological adaptation.

show abstract

Human Vascular Wall Microfluidic Model for Preclinical Evaluation of Drug-Induced Vascular Injury

Cited by 4 publications

References 47 publications

Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow

Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow

Investigation of the Dysfunction Caused by High Glucose, Advanced Glycation End Products, and Interleukin‐1 Beta and the Effects of Therapeutic Agents on the Microphysiological Artery Model

Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling

Contact Info

Product

Resources

About