Human Umbilical Vein Endothelial Cells Migration in Matrigel by the Concentration Gradient of Vascular Endothelial Growth Factor

Obi, Naoko; Toda, Hiroyuki; Tabata, Yasuhiko

doi:10.4172/2155-952x.1000210

Cited by 8 publications

(4 citation statements)

References 55 publications

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“…The dependency of cell migration to the VEGF content of the scaffolds was also studied by Obi et al [55]. They incorporated VEGF into a gelatin hydrogel sheet and put Matrigel seeded with HUVECs on these gelatin surfaces.…”

Section: Cell Migrationmentioning

confidence: 97%

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Jafarkhani¹,

Salehi²,

Mashayekhan³

et al. 2020

Biomed. Mater.

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Endothelial cell migration is a crucial step in the procedure of new blood vessel formation-a necessary process to maintain cell viability inside thick tissue constructs. Here, we report a new method for maintaining cell viability and inducing cell migration using a perfused microfluidic platform based on collagen gel and a gradient hydrogel sheet. Due to the helpful role of the extracellular matrix (ECM) components in cell viability, we developed a hydrogel sheet from decellularized tissue (DT) of the bovine heart and chitosan (CS). The results showed that the hydrogel sheets with an optimum weight ratio of CS/DT=2 possess a porosity of around 75%, a mechanical strength of 23 kPa, and display cell viability up to 78%. Then, we immobilized a radial gradient of vascular endothelial growth factor (VEGF) on the hydrogel sheet to promote human umbilical vein endothelial cells (HUVECs) migration. Finally, we incorporated the whole system as an entirety on the top of the microfluidic platform and studied cell migration through the hydrogel sheet in the presence of soluble and immobilized VEGF. The results demonstrated that immobilized VEGF stimulated cell migration in the hydrogel sheet at all depths compared with soluble VEGF. The results also showed that applying a VEGF gradient in both soluble and immobilized states had a significant effect on cell migration at limited depths (<100 μm). The main finding of this study is a significant improvement in cell migration using an in vivo imitating, costefficient, and highly reproducible platform, which may open up a new perspective for tissue engineering applications.

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Section: Cell Migrationmentioning

confidence: 97%

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Jafarkhani¹,

Salehi²,

Mashayekhan³

et al. 2020

Biomed. Mater.

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show abstract

“…It is largely bound to glycosaminoglycans in vivo and released in response to need. Its angiogenic potential has been assessed in many in vitro studies such as cell migration assays using matrigel [8], collagen gels [9] and transwell migration assays [10]. It has also been evaluated in the CAM assay [11 14] as well as in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

Exploration of 2-Deoxy-D-Ribose and 17β-Estradiol as Alternatives to Exogenous VEGF to Promote Angiogenesis in Tissue-Engineered Constructs

et al. 2019

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Aim:In this study, we explored the angiogenic potential and proangiogenic concentration ranges of 2deoxy-D-ribose (2dDR) and -Estradiol (E2) in comparison with vascular endothelial growth factor (VEGF). 2dDR and E2 were then loaded into tissue engineering (TE) scaffolds to investigate their proangiogenic potential when released from fibres. Materials and Methods: Ex-ovo chick chorioallantoic membrane (CAM) assay was used to evaluate angiogenic activity of 2dDR and E2. Both factors were then introduced into scaffolds via electrospinning to assess their angiogenic potential when released from fibres. Results: Both factors were approximately 80% as potent as (VEGF) and showed a dose-dependent angiogenic response. The sustained release of both agents from the scaffolds stimulated neovascularisation over 7 days in the CAM assay. Conclusion: We conclude that both 2dDR and E2 provide attractive alternatives to VEGF for the functionalisation of TE scaffolds to promote angiogenesis in vivo. Graphical abstract:

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“…Moreover, it had already been shown that endothelial cells, cultured in a proper matrix such as Matrigel, migrate in the direction of VEGF release. Accordingly, the gradient concentration of VEGF, formed in Matrigel, induces the migration of endothelial cell 42 …”

Section: Discussionmentioning

confidence: 99%

A preliminary study of endothelial cell migration during angiogenesis using a meshless method approach

Guerra

Belinha

Jorge

2020

Numer Methods Biomed Eng

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Angiogenesis, the development of new blood capillaries, is crucial for the wound healing process. This biological process allows the proper blood supply to the tissue, essential for cell proliferation and viability. Several biological factors modulate angiogenesis, however the vascular endothelial growth factor (VEGF) is the main one. Given the complexity of angiogenesis, in the last years, computational modelling aroused the interest of scientists since it allows to model this process with different, more economic and faster methodologies, comparatively to experimental approaches. In this work, a mathematical model motivated by the analysis of the effect of VEGF diffusion gradient in endothelial cell migration is presented. This is the process that allows capillary formation and it is essential for angiogenesis. The proposed mathematical model is combined with the Radial Point Interpolation Method, being the area discretized considering an unorganized nodal cloud and a background mesh of integration points, without predefined relations. The nodal connectivity was achieved using the “influence‐domain” approach. The interpolation functions were constructed using the Radial Point Interpolators techniques. This method combines a radial basis functions with a polynomial functions to obtain the approximation. This preliminary work does not account for the whole complexity of cell and tissue biology, and numerical results are presented for an idealised two‐dimensional setting. Nevertheless, the developed RPIM software is a valid numerical tool that can be adjusted to biological problems and may also be able to complement the biological and medical subjects.

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Human Umbilical Vein Endothelial Cells Migration in Matrigel by the Concentration Gradient of Vascular Endothelial Growth Factor

Cited by 8 publications

References 55 publications

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Exploration of 2-Deoxy-D-Ribose and 17β-Estradiol as Alternatives to Exogenous VEGF to Promote Angiogenesis in Tissue-Engineered Constructs

A preliminary study of endothelial cell migration during angiogenesis using a meshless method approach

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