Human umbilical cord Wharton’s jelly stem cells: Immune property genes assay and effect of transplantation on the immune cells of heart failure patients

Chen, Haiying; Zhang, Ningkun; Li, Tianchang; Guo, Jianhua; Wang, Zhiguo; Yang, Ming; Lian-ru, Gao

doi:10.1016/j.cellimm.2012.03.012

Cited by 26 publications

(15 citation statements)

References 45 publications

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“…Interestingly, other MSC populations, as cardiac-derived ones, do express both HLA-E and HLA-F molecules [76]. Another recent report by Chen and co-workers confirmed our original observations about the expression of all of three class Ib HLAs by WJ-MSC [44]. HLA-E expression has been also demonstrated in CL-MSC and BM-MSC, but only after challenge with TNF alpha (see Table 1) [40].…”

Section: Recent Data On the Expression Of Relevant Immunomodulatory Msupporting

confidence: 65%

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Rocca¹,

Corrao

Iacono

et al. 2012

TOTERMJ

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Mesenchymal (stromal) stem cells (MSC) are a broad class of stromal populations which are able to differentiate towards mature cell types, and do express molecules involved in immune modulation, tolerance induction and inflammation dampening. MSC can be virtually isolated from each adult organ, as well as from foetus-associated perinatal tissues. In particular, Wharton's jelly-derived MSC (WJ-MSC) bear all of these key properties, together with their ease of sourcing and lack of ethical issues.Cellular therapy is a key technique in regenerative medicine approaches, in particular for the treatment of diseases in which physiological processes of cellular repopulation are blocked by the underlying pathological conditions. Recent data enlightened the ability of administered cells to act also in a repopulation-independent fashion in target organs, since their peculiar immunomodulatory and anti-inflammatory features may favor organ self-repair by reactivating local progenitors by both cell-mediated or paracrine mechanisms. Translating classical regenerative medicine to "reparative medicine" or "support medicine" should represent a further therapeutic strategy independent from the differentiation capacity of MSC populations.Recent data further highlighted that WJ-MSC outperform BM-MSC (which are now being applied clinically) both in terms of immune modulation and lack of tumorigenesis (or tumor-promoting activities) in vivo. Starting from these premises, this paper analyzes the recent data on the biology of WJ-MSC, considering the role of both naïve and differentiated cells in immune modulation. In particular, the role of tolerance promoting pathways via non-classical B7 costimulators or class Ib MHC molecules are examined. In addition, we also analyzed the interconnections with other mechanicistic pathways (as those driven by matrix degrading metalloproteinases) in immune modulation. Our observations strongly support the notion that WJ-MSC may constitute a new tool in regenerative and reparative medicine applications.

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Section: Recent Data On the Expression Of Relevant Immunomodulatory Msupporting

confidence: 65%

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Rocca¹,

Corrao

Iacono

et al. 2012

TOTERMJ

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“…Our previous studies have presented biological characteristics of WJ‐MSCs, such as morphological and immunophenotypic characterization (Chen et al, ; Gao et al, ). Our experiments showed that transfection efficiency and proliferation features of WJ‐MSCs transfected by apelin‐siRNA and apelin‐overexpression lentiviral vectors resembled those of negative lentiviral vector.…”

Section: Resultsmentioning

confidence: 99%

Apelin: an endogenous peptide essential for cardiomyogenic differentiation of mesenchymal stem cells via activating extracellular signal‐regulated kinase 1/2 and 5

Wang

Zhu

Zhang

et al. 2016

Cell Biology International

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Growing evidence has shown that apelin/APJ system functions as a critical mediator of cardiac development as well as cardiovascular function. Here, we investigated the role of apelin in the cardiomyogenic differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord in vitro. In this research, we used RNA interference methodology and gene transfection technique to regulate the expression of apelin in Wharton's jelly-derived mesenchymal stem cells and induced cells with a effective cardiac differentiation protocol including 5-azacytidine and bFGF. Four weeks after induction, induced cells assumed a stick-like morphology and myotube-like structures except apelin-silenced cells and the control group. The silencing expression of apelin in Wharton's jelly-derived mesenchymal stem cells decreased the expression of several critical cardiac progenitor transcription factors (Mesp1, Mef2c, NKX2.5) and cardiac phenotypes (cardiac α-actin, β-MHC, cTnT, and connexin-43). Meanwhile, endogenous compensation of apelin contributed to differentiating into cells with characteristics of cardiomyocytes in vitro. Further experiment showed that exogenous apelin peptide rescued the cardiomyogenic differentiation of apelin-silenced mesenchymal stem cells in the early stage (1-4 days) of induction. Remarkably, our experiment indicated that apelin up-regulated cardiac specific genes in Wharton's jelly-derived mesenchymal stem cells via activating extracellular signal-regulated kinase (ERK) 1/2 and 5.

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“…The isolation and culture of MSCs from individual cord regions including cord vein and the perivascular region [7, 10, 13, 14], Wharton's jelly [11], and cord lining [12] has been reported by many groups. However, none of these studies or indeed any other studies have compared all cord regions from within the same individual [1].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterisation of Mesenchymal Stem Cells from Different Regions of the Human Umbilical Cord

Mennan

Wright

Bhattacharjee

et al. 2013

BioMed Research International

120

107

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Umbilical cords as a source of stem cells are of increasing interest for cell therapies as they present little ethical consideration and are reported to contain immune privileged cells which may be suitable for allogeneic based therapies. Mesenchymal stem cells (MSCs) sourced from several different cord regions, including artery, vein, cord lining, and Wharton's jelly, are described in the literature. However, no one study has yet isolated and characterised MSCs from all regions of the same cord to determine the most suitable cells for cell based therapeutics.

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Human umbilical cord Wharton’s jelly stem cells: Immune property genes assay and effect of transplantation on the immune cells of heart failure patients

Cited by 26 publications

References 45 publications

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Apelin: an endogenous peptide essential for cardiomyogenic differentiation of mesenchymal stem cells via activating extracellular signal‐regulated kinase 1/2 and 5

Isolation and Characterisation of Mesenchymal Stem Cells from Different Regions of the Human Umbilical Cord

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