Human umbilical cord mesenchymal stem cells as treatment of adjuvant rheumatoid arthritis in a rat model

Greish, Sahar M; Abogresha, Noha M.; Abdel-Hady, Zeinab; Zakaria, Eman; Ghaly, Mona; Hefny, Mohamed

doi:10.4252/wjsc.v4.i10.101

Cited by 45 publications

(33 citation statements)

References 30 publications

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“…Therefore, MSCs may modulate the immune response and induce anti-inflammatory effects [34,35,36]. Furthermore, the availability of MSCs for autologous and allogeneic transplantation makes them useful in the treatment of SCI.…”

Section: Resultsmentioning

confidence: 99%

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Urdzíková

Růžička

LaBagnara

et al. 2014

IJMS

102

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Transplantation of mesenchymal stem cells (MSC) improves functional recovery in experimental models of spinal cord injury (SCI); however, the mechanisms underlying this effect are not completely understood. We investigated the effect of intrathecal implantation of human MSC on functional recovery, astrogliosis and levels of inflammatory cytokines in rats using balloon-induced spinal cord compression lesions. Transplanted cells did not survive at the lesion site of the spinal cord; however, functional recovery was enhanced in the MSC-treated group as was confirmed by the Basso, Beattie, and Bresnahan (BBB) and the flat beam test. Morphometric analysis showed a significantly higher amount of remaining white matter in the cranial part of the lesioned spinal cords. Immunohistochemical analysis of the lesions indicated the rearrangement of the glial scar in MSC-treated animals. Real-time PCR analysis revealed an increased expression of Irf5, Mrc1, Fgf2, Gap43 and Gfap. Transplantation of MSCs into a lesioned spinal cord reduced TNFα, IL-4, IL-1β, IL-2, IL-6 and IL-12 and increased the levels of MIP-1α and RANTES when compared to saline-treated controls. Intrathecal implantation of MSCs reduces the inflammatory reaction and apoptosis, improves functional recovery and modulates glial scar formation after SCI, regardless of cell survival. Therefore, repeated applications may prolong the beneficial effects induced by MSC application.

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Section: Resultsmentioning

confidence: 99%

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Urdzíková

Růžička

LaBagnara

et al. 2014

IJMS

102

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“…91,92 Besides BM, MSCs can be isolated from adipose tissues, lung, gingiva, placenta, liver, cord blood, and umbilical cord. [93][94][95][96] There are some similar experiments showing that MSCs from the other tissues also can polarize macrophages to the alternatively activated M2 phenotype. In parallel observation with human placental MSCs, Abumaree et al 97 also found that the human placental MSCs also have potent anti-inflammatory and immunosuppressive actions via converting macrophages from a proinflammatory M1 phenotype to an anti-inflammatory M2 phenotype.…”

mentioning

confidence: 77%

Anti-inflammatory effect of stem cells against spinal cord injury via regulating macrophage polarization

Zhang¹,

He²

2017

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Spinal cord injury (SCI) is a traumatic event that involves not just an acute physical injury but also inflammation-driven secondary injury. Macrophages play a very important role in secondary injury. The effects of macrophages on tissue damage and repair after SCI are related to macrophage polarization. Stem cell transplantation has been studied as a promising treatment for SCI. Recently, increasing evidence shows that stem cells, including mesenchymal stem, neural stem/progenitor, and embryonic stem cells, have an anti-inflammatory capacity and promote functional recovery after SCI by inducing macrophages M1/M2 phenotype transformation. In this review, we will discuss the role of stem cells on macrophage polarization and its role in stem cell-based therapies for SCI.

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“…Lymphocytes and monocytes generally secrete this cytokine; however, other PBMCs can synthesize and release IL-10 [32] . Recent in vivo studies have observed an increased level of IL-10 after transplanting MSCs or injecting the cell-free medium of these cells to animal models [33][34][35] . Although cell-cell interactions between MSCs and different cells of the immune system play a significant role in immune-modulation, the secretion of soluble factors is more important to suppress the immune system [6] .…”

Section: Discussionmentioning

confidence: 99%

Hypoxia Pre-Conditioned Embryonic Mesenchymal Stem Cell Secretome Reduces IL-10 Production by Peripheral Blood Mononuclear Cells

Lotfinia

Lak

Ghahhari

et al. 2017

IBJ

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Background: Mesenchymal stem cells (MSCs) are important candidates for MSC-based cellular therapy. Current paradigm states that MSCs support local progenitor cells in damaged tissue through paracrine signaling. Therefore, the study of paracrine effects and secretome of MSCs could lead to the appreciation of mechanisms and molecules associated with the therapeutic effects of these cells. This study analyzed anti-inflammatory and immune-modulatory effects of MSC secretomes derived from embryonic stem cells (ESCs) and bone marrow cells after hypoxia and normoxia preconditioning. Methods: ESCs differentiated into MSCs and characterized by flow cytometry as well as by differentiation into adipocytes and osteoblasts. The experimental groups were consisted of individual groups of ESC-MSCs and BM-MSCs (bone marrow-derived mesenchymal stromal cells), which were preconditioned with either hypoxia or normoxia for 24, 48 and 72 h. After collecting the cell-free medium from each treatment, secretomes were concentrated by centrifugal filters. Using a peripheral blood mononuclear cell (PBMC) assay and ELISA, IL-10 concentration in PBMCs was evaluated after their incubation with different secretomes from preconditioned and non-preconditioned MSCs. Results: A significant difference was observed between ESC-MSC normoxia and ESC-MSC hypoxia in IL-10 concentration, and normoxia secretomes increased IL-10 secretion from PBMCs. Moreover, the strongest IL-10 secretion from PBMCs could be detected after the stimulation by ESC-MSC conditioned secretomes, but not BM-MSC conditioned medium. Conclusions: Human hypoxia preconditioned ESC-MSC secretome indicated stronger immune-modulatory effects compared to BM-MSC conditioned medium. It could be suggested that induced MSCs confer less immune-modulatory effects but produce more inflammatory molecules such as tumor necrosis factor α, which needs further investigation.

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Human umbilical cord mesenchymal stem cells as treatment of adjuvant rheumatoid arthritis in a rat model

Abstract: MSC enhance the efficacy of CFA-induced arthritis treatment, most likely through the modulation of the expression of cytokines and amelioration of pathological changes in joints.

Cited by 45 publications

References 30 publications

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Anti-inflammatory effect of stem cells against spinal cord injury via regulating macrophage polarization

Hypoxia Pre-Conditioned Embryonic Mesenchymal Stem Cell Secretome Reduces IL-10 Production by Peripheral Blood Mononuclear Cells

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