Human umbilical cord mesenchymal stem cells improve morphometric and histopathologic changes of cyclophosphamide-injured ovarian follicles in mouse model of premature ovarian failure

Jalalie, Ladan; Rezaee, Mohammad Ali; Rezaie, Mohammad Jafar; Jalili, Ali; Raoofi, Amir; Rustamzade, Auob

doi:10.1016/j.acthis.2020.151658

Cited by 12 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study discovered that CPA administration causes POF by worsening follicular atresia and impaired hormonal functions, which are associated with several mechanisms, including oxidative stress with lipid peroxidation, inflammation, and apoptosis of the ovarian follicles. These results were in agreement with those who noticed that CPA significantly increased LH and FSH together with deceasing estradiol and progesterone levels in POF rats [19,20]. The presence of these hormonal alterations is utilized as a sign of ovarian failure [21].…”

Section: Discussionsupporting

confidence: 93%

Ameliorative Effect of Citrus Lemon Peel Extract and Resveratrol on Premature Ovarian Failure Rat Model: Role of iNOS/Caspase-3 Pathway

et al. 2022

View full text Add to dashboard Cite

Premature ovarian failure (POF) is described as a loss of oocytes and the absence of folliculogenesis and is considered an adverse effect of chemotherapeutic drugs, which leads to infertility. Subsequently, the existing inquiry was achieved by exploring the potential suspicious influences of lemon peel extract (LPE), and resveratrol (RES) on cyclophosphamide (CPA) induced-POF. The results showed that CPA-induced POF significantly decreased serum estradiol (E2) and progesterone levels, along with a considerable rise in serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels. Moreover, CPA administration to rats significantly increased the serum level of Malondialdehyde (MDA) and significantly lowered the levels of reduced glutathione (GSH) and superoxide dismutase (SOD); in addition, it increased nuclear factor kappa B (NF-κB) levels, tumor necrosis factor-α (TNF-α), as well as cyclooxygenase 2 (COX-2) with the spread expression of inducible nitric oxide synthase (iNOS) mRNA levels and caspase-3 (Casp3) levels in ovarian tissues versus the control rats. However, treatment with LPE and RES suppressed the triggering of NF- κB pathways, evidenced by a considerable reduction in Casp3 & iNOS mRNA expression level and significant ameliorative effects in all evaluated parameters, as confirmed by the histological and immunohistochemical investigation when comparing the model group. In overall findings, both lemon peel extract and resveratrol can mitigate the adverse effects of CPA-induced POF. Most crucially, its combination therapy is a promising pharmacological agent for this disease.

show abstract

Section: Discussionsupporting

confidence: 93%

Ameliorative Effect of Citrus Lemon Peel Extract and Resveratrol on Premature Ovarian Failure Rat Model: Role of iNOS/Caspase-3 Pathway

et al. 2022

View full text Add to dashboard Cite

show abstract

“…We extracted the data on the first author, country, publication year, experiment animal number and situation, model establishment situation, group situation, some outcomes and web link. We included nine studies from China ( 23 , 24 , 26 , 28 – 33 ), one from Saudi Arabia ( 25 ) and one from Iran ( 27 ). A total of 158 stem cell–treated animals and 155 POI model animals were included.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic prospect on umbilical cord mesenchymal stem cells in animal model with primary ovarian insufficiency: a meta-analysis

Wang

Bai

et al. 2023

Front. Med.

View full text Add to dashboard Cite

BackgroundPrimary ovarian insufficiency (POI) leads to not only infertile but several adverse health events to women. Traditional treatment methods have their own set of limitations and drawbacks that vary in degree. Application of human umbilical cord mesenchymal stem cell (hUCMSC) is a promising strategy for POI. However, there is a lack of literatures on application of hUCMSC in human. Animal experimental model, however, can reflect the potential effectiveness of this employment. This study aimed to evaluate the curative effect of hUCMSC on animals with POI on a larger scale.MethodsTo gather data, Pubmed, Embase, and Cochrane Library were searched for studies published up to April 2022. Various indices, including the animals' estrous cycle, serum sex hormone levels, and follicle number in the ovary, were compared between the experimental group and those with Premature Ovarian Insufficiency (POI).ResultsThe administration of human umbilical cord-derived mesenchymal stem cells (hUCMSC) has been shown to significantly improve the estrous cycle (RR: 3.32, 95% CI: [1.80, 6.12], I2 = 0%, P = 0.0001), but robustly decrease its length (SMD: −1.97, 95% CI: [−2.58, −1.36], I2 = 0%, P < 0.00001). It can also strikingly increase levels of serum estradiol (SMD: 5.34, 95% CI: [3.11, 7.57], I2 = 93%, P < 0.00001) and anti-müllerian hormone (SMD: 1.92, 95% CI: [0.60, 3.25], I2 = 68%, P = 0.004). Besides, it lowers levels of serum follicle-stimulating hormone (SMD: −3.02, 95% CI: [−4.88, −1.16], I2 = 93%, P = 0.001) and luteinising hormone (SMD: −2.22, 95% CI: [−3.67, −0.76], I2 = 78%, P = 0.003), and thus collectively promotes folliculogenesis (SMD: 4.90, 95% CI: [3.92, 5.88], I2 = 0%, P < 0.00001).ConclusionsBased on the presented findings, it is concluded that the administration of hUCMSC in animal models with POI can result in significant improvements in several key indicators, including estrous cycle recovery, hormone level modulation, and promotion of folliculogenesis. These positive outcomes suggest that hUCMSC may have potential as a treatment for POI in humans. However, further research is needed to establish the safety and efficacy of hUCMSC in humans before their clinical application.Systematic review registrationhttps://inplasy.com/inplasy-2023-5-0075/, identifier: INPLASY202350075.

show abstract

“…Another study demonstrated the molecular signaling pathways involved in therapeutic effects of MSCs in ovarian failure. These pathways include anti-apoptosis, angiogenesis, trans-differentiation, immune-regulation, antioxidant effects, anti-brosis, and cell migration [32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Use of mesenchymal stem cells in Experimental Ovarian Damage

Fouad¹,

Albahlol

Wahab³

et al. 2022

Preprint

View full text Add to dashboard Cite

The study was conducted to evaluate the therapeutic potential of mesenchymal stem cells (MSCs) in Bisphenol A (BPA) -induced ovarian damage. The study was conducted on 50 mature female rats divided into: Group 1; control group, Group IIa, IIb: BPA group received oral gavage of BPA with two doses of 25, 50 mg/Kg body weight daily for 15 days, Group IIIa, IIIb; MSCs group intravenously injected with a single dose of MSCs (106 cells) after the last dose of BPA. Blood and ovarian tissue levels of Malondialdehyde (MDA) and gonadal axis hormones were assessed. Apoptosis was evaluated by TUNNEL assay and by apoptosis markers (FAS, FASL, Caspase 3, SLTM). Histological examination of ovarian tissue was assessed. Histological examination of ovarian tissues revealed that at high dose of BPA, there was significant increase in the number of atretic follicles whereas the numbers of primordial, primary, secondar, graafian follicles were significantly decreased. Moreover, all genetic and protein markers of apoptosis were significantly elevated in BPA treated rat groups with decreased estrogen receptor expression in the ovarian tissue. Results also showed significant elevation in plasma levels of LH and FSH, ovarian tissue levels of MDA, significant decrease in estradiol (E2), progesterone. Increased apoptotic cells were confirmed by TUNEL assay. All histological and laboratory abnormalities were significantly ameliorated after treatment with MSCs. In conclusion, follicles atresia is caused by BPA through upregulated expression of apoptosis related genes. MSCs induced significant amelioration of BPA induced-ovarian damage as regards apoptosis markers, biochemical and gonadal axis hormones.

show abstract

Human umbilical cord mesenchymal stem cells improve morphometric and histopathologic changes of cyclophosphamide-injured ovarian follicles in mouse model of premature ovarian failure

Cited by 12 publications

References 27 publications

Ameliorative Effect of Citrus Lemon Peel Extract and Resveratrol on Premature Ovarian Failure Rat Model: Role of iNOS/Caspase-3 Pathway

Ameliorative Effect of Citrus Lemon Peel Extract and Resveratrol on Premature Ovarian Failure Rat Model: Role of iNOS/Caspase-3 Pathway

Therapeutic prospect on umbilical cord mesenchymal stem cells in animal model with primary ovarian insufficiency: a meta-analysis

Use of mesenchymal stem cells in Experimental Ovarian Damage

Contact Info

Product

Resources

About