Human umbilical cord mesenchymal stem cell-derived exosomal microRNA-148a-3p inhibits neointimal hyperplasia by targeting Serpine1

Zhang, Xiaoyu; Zhou, Yu; Ye, Yanchen; Wu, Ridong; Li, Wen; Yao, Chen; Wang, Shenming

doi:10.1016/j.abb.2022.109155

Cited by 5 publications

(3 citation statements)

References 53 publications

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“…Some research results have been applied in a variety of clinical trials, such as malignant tumors, autoimmune system diseases, nervous system diseases, and cardiovascular diseases [5][6][7]. Human umbilical cord mesenchymal stem cells (HUCMSCs) have achieved the results in the clinical treatment research of lupus nephritis, rheumatoid arthritis, diabetes, neuropathy, decompensated cirrhosis, liver failure, and other diseases [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure through Cell Apoptosis of miR-100-5p/NOX4/NLRP3

Niu

Yang

Luo

et al. 2022

BioMed Research International

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Premature ovarian failure refers to a series of symptoms of perimenopausal hot flashes, night sweats, decreased libido, vaginal dryness, insomnia, reduced menstruation, sparse hair, even amenorrhea, and even infertility before the age of 40 due to the decline of ovarian function. Premature ovarian failure is a common and difficult disease in gynecology. Its prevalence is increasing gradually, and the trend is younger. The aim of this experiment was to elucidate the role of human umbilical cord mesenchymal stem cells (HUCMSCs) in premature ovarian failure and its mechanism. HUCMSCs, KGN cells, and HEK293T cells were used in this experiment. Quantitative PCR and microarray analysis, ELISA inflammation and oxidative stress kits, RNA pull-down assay, luciferase reporter assay, proliferation assay, EDU staining, and Western blot analysis were used. In an in vitro model of premature ovarian failure, HUCMSCs attenuated inflammatory response, oxidative stress, and apoptosis. HUCMSCs ameliorated the premature ovarian failure model. The miR-100-5p expression was induced by HUCMSCs through methylation. miR-100-5p regulation influenced the role of HUCMSCs in an in vitro model of premature ovarian failure. HUCMSCs inhibited the in vitro expression of NOX4, NLRP3, and GSDMD proteins in the model. NOX4/NLRP3 signaling pathway affects the role of HUCMSCs in an in vitro model of premature ovarian failure through miR-100-5p. This experiment elucidated the role of HUCMSCs in premature ovarian failure and its mechanism, with a view to providing a clinical reference.

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Section: Introductionmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure through Cell Apoptosis of miR-100-5p/NOX4/NLRP3

Niu

Yang

Luo

et al. 2022

BioMed Research International

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“…The mRNA-seq data were analysed as previously described. 38 First, the raw data were filtered with SOAPnuke (v1.4.0) before the reliability analysis was performed. Next, we used HISAT (v2.1.0) to align the clean reads to the reference genome.…”

Section: Mrna Sequencing (Mrna-seq) Analysismentioning

confidence: 99%

Pterostilbene alleviates abdominal aortic aneurysm via inhibiting macrophage pyroptosis by activating the miR-146a-5p/TRAF6 axis

Cai,

Huang,

Wang

et al. 2024

Food Funct.

Self Cite

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“…MiR-148a-3p was improved in hUC-MSC-EXOs and inhibited Serpine1 via direct binding to its 3′-UTR area. Furthermore, hUC-MSC-EXOs miR-148a-3p inhibited VSMC phenotypic switching and migration via targeting Serpine1 [ 142 ]. The separated UC-MSC-EXOs had a characteristic cup-formed morphology, expressed the particular exosomal markers Alix, CD63, and TSG101, and were about 50–150 nm in size.…”

Section: Different Types Of Msc-exos As Mirnas Delivery Systems In Di...mentioning

confidence: 99%

The potential use of mesenchymal stem cells-derived exosomes as microRNAs delivery systems in different diseases

Oveili¹,

Vafaei

Bazavar

et al. 2023

Cell Commun Signal

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MicroRNAs (miRNAs) are a group of small non-coding RNAs that regulate gene expression by targeting mRNA. Moreover, it has been shown that miRNAs expression are changed in various diseases, such as cancers, autoimmune disease, infectious diseases, and neurodegenerative Diseases. The suppression of miRNA function can be easily attained by utilizing of anti-miRNAs. In contrast, an enhancement in miRNA function can be achieved through the utilization of modified miRNA mimetics. The discovery of appropriate miRNA carriers in the body has become an interesting subject for investigators. Exosomes (EXOs) therapeutic efficiency and safety for transferring different cellular biological components to the recipient cell have attracted significant attention for their capability as miRNA carriers. Mesenchymal stem cells (MSCs) are recognized to generate a wide range of EXOs (MSC-EXOs), showing that MSCs may be effective for EXO generation in a clinically appropriate measure as compared to other cell origins. MSC-EXOs have been widely investigated because of their immune attributes, tumor-homing attributes, and flexible characteristics. In this article, we summarized the features of miRNAs and MSC-EXOs, including production, purification, and miRNA loading methods of MSC-EXOs, and the modification of MSC-EXOs for targeted miRNA delivery in various diseases. Graphical abstract

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Human umbilical cord mesenchymal stem cell-derived exosomal microRNA-148a-3p inhibits neointimal hyperplasia by targeting Serpine1

Cited by 5 publications

References 53 publications

Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure through Cell Apoptosis of miR-100-5p/NOX4/NLRP3

Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure through Cell Apoptosis of miR-100-5p/NOX4/NLRP3

Pterostilbene alleviates abdominal aortic aneurysm via inhibiting macrophage pyroptosis by activating the miR-146a-5p/TRAF6 axis

The potential use of mesenchymal stem cells-derived exosomes as microRNAs delivery systems in different diseases

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