Human umbilical cord-derived mesenchymal stromal cells alleviate liver cirrhosis through the Hippo/YAP/Id1 pathway and macrophage-dependent mechanism

Yao, Lichao; Han, Xue; Yuan, Man; Liu, Pingji; Zhang, Qiuling; Zheng, Wei; Chen, Ping; Xiong, Zhiyu; Wu, Lun; Dai, Kai; Jiang, Yingan

doi:10.1016/j.intimp.2023.110456

Cited by 3 publications

(3 citation statements)

References 45 publications

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“…KEGG analysis implicated ID1 and SNAI2 in the Hippo signaling pathway. ID1, SNAI2 and the Hippo pathway are linked to various diseases, with the latter particularly associated with brosis 25 and macrophage-dependent mechanisms, alleviating liver cirrhosis in mice 29 .Therefore, we propose ID1 and SNAI2's involvement in AF occurrence and development through the Hippo signaling pathway.…”

Section: Disscussionmentioning

confidence: 82%

Crosstalk between Atrial Cardiomyocytes and Epicardial Adipose Tissue in Atrial Fibrillation: Insights from Machine Learning Methods and Rat Atrial Fibrillation Model

Zhang,

Miao,

Song

et al. 2024

Preprint

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Objective: Investigating the Role of Epicardial Adipose Tissue as a Catalyst for Reversal and Reconstruction of Atrial Myocardial Cells in the Context of Dialogue with Atrial Myocardial Cells. Implications for Breakthroughs in Preventing Paroxysmal Atrial Fibrillation Progression. Methods: We obtained three datasets (GSE41177, GSE31821, and GSE135455) associated with atrial fibrillation (AF) from the Gene Expression Omnibus (GEO) database, which were subsequently merged for comprehensive analysis. Differentially expressed genes (DEGs) were identified using the "limma" package in the R software. Candidate AF genes were selected through machine learning techniques, including the LASSO regression algorithm and SVM-RFE algorithm. The diagnostic efficacy of these genes was evaluated using Receiver Operating Characteristic (ROC) curves. Additionally, CIBERSORT was employed to investigate the proportions of infiltrating immune cells in each sample, while the Pearson method was applied to examine the correlation between genes and immune cells. Further validation of the DEGs were performed by PCR in atrial fibrillation rats. Results: A total of 310 Differentially Expressed Genes (DEGs) were identified in atrial cardiomyocytes with epicardial adipose tissue. Using the LASSO regression and SVM-RFE algorithms, ID1, SCN4A, COL4A5, COLEC11, and SNAI2 were pinpointed as key genes associated with Atrial Fibrillation (AF). In both the training and validation datasets, these genes exhibited robust effectiveness. The immune infiltration analysis revealed that, in comparison to sinus rhythm (SR), atrial samples from patients with AF exhibited higher levels of neutrophils, while T cells follicular helper were relatively lower. Correlation analysis highlighted significant associations between ID1, SCN4A, COL4A5, COLEC11, SNAI2, and infiltrating immune cells. The outcomes of the RT- qPCR analysis in our investigation were consistent with the findings of bioinformatics analysis. Conclusions: In summary, this study posits that ID1, SCN4A, COL4A5, COLEC11, and SNAI2 emerge as pivotal genes in Atrial Fibrillation (AF), exhibiting correlation with infiltrating immune cells. Furthermore, it underscores the indispensable roles played by infiltrating immune cells in the context of AF.

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Section: Disscussionmentioning

confidence: 82%

Crosstalk between Atrial Cardiomyocytes and Epicardial Adipose Tissue in Atrial Fibrillation: Insights from Machine Learning Methods and Rat Atrial Fibrillation Model

Zhang,

Miao,

Song

et al. 2024

Preprint

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“…The hUCMSCs were purchased from Shenzhen Wingor Biotechnology Co., Ltd [ 20 ]. Moreover, the cells used in the experiments were obtained from 3-6 generations of the same batch of cells.…”

Section: Methodsmentioning

confidence: 99%

Human umbilical cord mesenchymal stem cells promote steroid-induced osteonecrosis of the femoral head repair by improving microvascular endothelial cell function

Chen,

Jin,

Zhong

et al. 2024

Aging

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Recently, there has been growing interest in using cell therapy through core decompression (CD) to treat osteonecrosis of the femoral head (ONFH). Our study aimed to investigate the effectiveness and mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) in treating steroid-induced ONFH. We constructed a steroid-induced ONFH rabbit model as well as dexamethasone (Dex)-treated bone microvascular endothelial cells (BMECs) model of human femoral head. We injected hUCMSCs into the rabbit femoral head via CD. The effects of hUCMSCs on steroid-induced ONFH rabbit model and Dex-treated BMECs were evaluated via micro-CT, microangiography, histology, immunohistochemistry, wound healing, tube formation, and western blotting assay. Furthermore, we conducted single-cell RNA sequencing (scRNA-seq) to examine the characteristics of endothelial cells, the activation of signaling pathways, and inter-cellular communication in ONFH. Our data reveal that hUCMSCs improved the femoral head microstructure and bone repair and promoted angiogenesis in the steroid-induced ONFH rabbit model. Importantly, hUCMSCs improved the migration ability and angioplasty of Dex-treated BMECs by secreting COL6A2 to activate FAK/PI3K/AKT signaling pathway via integrin α1β1.

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“…Mice were injected intraperitoneally with CCl4 (1 mL/kg, twice a week, for 12 weeks) to induce liver cirrhosis. 18 Control mice were injected with the same volume and frequency of PBS. After the model was established, blood, liver, and fecal specimens were collected from all mice.…”

Section: Methodsmentioning

confidence: 99%

Causal Relationship Between Gut Microbiota and Liver Cirrhosis: 16S rRNA Sequencing and Mendelian Randomization Analyses

Yuan,

Hu,

Yao

et al. 2023

J Clin Transl Hepatol

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Background and Aims Accumulating evidence highlights the association between the gut microbiota and liver cirrhosis. However, the role of the gut microbiota in liver cirrhosis remains unclear. Methods We first assessed the differences in the composition of the bacterial community between CCl4-induced liver cirrhosis and control mice using 16S rRNA sequencing. We then performed a two-sample Mendelian randomization (MR) analysis to reveal the underlying causal relationship between the gut microbiota and liver cirrhosis. Causal relationships were analyzed using primary inverse variance weighting (IVW) and other supplemental MR methods. Furthermore, fecal samples from liver cirrhosis patients and healthy controls were collected to validate the results of the MR analysis. Results Analysis of 16S rRNA sequencing indicated significant differences in gut microbiota composition between the cirrhosis and control groups. IVW analyses suggested that Alphaproteobacteria, Bacillales, NB1n, Rhodospirillales, Dorea , Lachnospiraceae , and Rhodospirillaceae were positively correlated with the risk of liver cirrhosis, whereas Butyricicoccus , Hungatella , Marvinbryantia , and Lactobacillaceae displayed the opposite effects. However, the weighted median and MR-PRESSO estimates further showed that only Butyricicoccus and Marvinbryantia presented stable negative associations with liver cirrhosis. No significant heterogeneity or horizontal pleiotropy was observed in the sensitivity analysis. Furthermore, the result of 16S rRNA sequencing also showed that healthy controls had a higher relative abundance of Butyricicoccus and Marvinbryantia than liver cirrhosis patients. Conclusions Our study provides new causal evidence for the link between gut microbiota and liver cirrhosis, which may contribute to the discovery of novel strategies to prevent liver cirrhosis.

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Human umbilical cord-derived mesenchymal stromal cells alleviate liver cirrhosis through the Hippo/YAP/Id1 pathway and macrophage-dependent mechanism

Cited by 3 publications

References 45 publications

Crosstalk between Atrial Cardiomyocytes and Epicardial Adipose Tissue in Atrial Fibrillation: Insights from Machine Learning Methods and Rat Atrial Fibrillation Model

Crosstalk between Atrial Cardiomyocytes and Epicardial Adipose Tissue in Atrial Fibrillation: Insights from Machine Learning Methods and Rat Atrial Fibrillation Model

Human umbilical cord mesenchymal stem cells promote steroid-induced osteonecrosis of the femoral head repair by improving microvascular endothelial cell function

Causal Relationship Between Gut Microbiota and Liver Cirrhosis: 16S rRNA Sequencing and Mendelian Randomization Analyses

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