Human Umbilical Cord Blood Serum/Plasma: Cytokine Profile and Prospective Application in Regenerative Medicine

Romanov, Yu. A.; Вторушина, В В; Dugina, T. N.; Romanov, Romanov A.Yu.; Petrova, Nadya

doi:10.1007/s10517-019-04670-2

Cited by 18 publications

(11 citation statements)

References 25 publications

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“…In our culture media, we used human umbilical cord plasma, which contained a mixture of growth factors, such as insulin-like growth factor-1, EGF, bFGF, and cytokines [ 43 , 44 ]. However, 2% of human umbilical cord plasma could not support full spermatogenesis from infant testicular tissue.…”

Section: Discussionmentioning

confidence: 99%

Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism

Wang

Hildorf

Ntemou

et al. 2022

IJMS

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Organotypic culture of human fetal testis has achieved fertilization-competent spermatids followed by blastocysts development. This study focuses on whether the organotypic culture of testicular tissue from infant boys with cryptorchidism could support the development of spermatogonia and somatic cells. Frozen-thawed tissues were cultured in two different media, with or without retinoic acid (RA), for 60 days and evaluated by tissue morphology and immunostaining using germ and somatic cell markers. During the 60-day culture, spermatocytes stained by boule-like RNA-binding protein (BOLL) were induced in biopsies cultured with RA. Increased AR expression (p < 0.001) and decreased AMH expression (p < 0.001) in Sertoli cells indicated advancement of Sertoli cell maturity. An increased number of SOX9-positive Sertoli cells (p < 0.05) was observed, while the percentage of tubules with spermatogonia was reduced (p < 0.001). More tubules with alpha-smooth muscle actin (ACTA, peritubular myoid cells (PTMCs) marker) were observed in an RA-absent medium (p = 0.02). CYP17A1/STAR-positive Leydig cells demonstrated sustained steroidogenic function. Our culture conditions support the initiation of spermatocytes and enhanced maturation of Sertoli cells and PTMCs within infant testicular tissues. This study may be a basis for future studies focusing on maintaining and increasing the number of spermatogonia and identifying different factors and hormones, further advancing in vitro spermatogenesis.

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Section: Discussionmentioning

confidence: 99%

Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism

Wang

Hildorf

Ntemou

et al. 2022

IJMS

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“…Human umbilical cord serum contains higher concentrations of multiple cytokines and growth factors than peripheral blood serum, and these cytokines and growth factors have anti-inflammatory and antiapoptotic effects. 21 Exosomes derived from umbilical cord blood stem cells have been reported to ameliorate IL-6-mediated acute liver injury. 22 Human umbilical cord serum can reduce gentamicin-induced hepatotoxicity by restoring peripheral oxidative damage and the inflammatory response in rats.…”

Section: Discussionmentioning

confidence: 99%

“…There has been little research on the relationship between umbilical cord blood and liver‐related diseases, let alone liver cancer. Human umbilical cord serum contains higher concentrations of multiple cytokines and growth factors than peripheral blood serum, and these cytokines and growth factors have anti‐inflammatory and antiapoptotic effects 21 . Exosomes derived from umbilical cord blood stem cells have been reported to ameliorate IL‐6‐mediated acute liver injury 22 .…”

Section: Discussionmentioning

confidence: 99%

Tubulin‐binding peptide RR‐171 derived from human umbilical cord serum displays antitumor activity against hepatocellular carcinoma via inducing apoptosis and activating the NF‐kappa B pathway

et al. 2022

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Objectives Hepatocellular carcinoma (HCC) still presents a high incidence of malignant tumours with poor prognosis. There is an urgent need for new therapeutic agents with high specificity, low toxicity and favourable solubility for the clinical treatment of HCC. Materials and Methods The bioactivity of human umbilical cord serum was investigated by proteomics biotechnology and a primitive peptide with certain biological activity was identified. The antitumour effect of RR‐171 was detected by cell viability assay in vitro, and determined by subcutaneous xenograft models assay and miniPDX assay in vivo. Pull‐down experiments were conducted to identify the potential targeting proteins of RR‐171. Immunofluorescence assay and tubulin polymerization assay were conducted to explore the relationship between RR‐171 and α‐tubulin. Fluorescence imaging in xenograft models was used to explore the biodistribution of RR‐171 in vivo. A phosphospecific protein microarray was performed to uncover the underlying signalling pathway by which RR‐171 induces tumour cell death. Results The results indicated that RR‐171 could be effective in the treatment of HCC in vivo and in vitro. RR‐171 could aggregate significantly in solid tumours and had no obvious systemic toxicity in vivo. RR‐171 could interact with α‐tubulin and activate the NF‐Kappa B pathway in HCC cells. Conclusions Taken together, RR‐171 exhibited significant antitumour activity against HCC in vivo and in vitro and could potentially be used in the clinical application of HCC.

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“…In this context, recent studies have focused on using the acellular fractions of the hUC blood, namely the human umbilical cord serum (hUCS) and plasma (hUCP) (Figure 1B). Since their GFs and cytokines produced comparable anti-inflammatory, angiogenic, and anti-apoptotic effects, the main difference between both biofluids is the absence (hUCS) or presence (hUCP) of clotting factors [114,115]. In addition, since hUCS and hUCP resemble the 'culture medium' of hUC blood cells, it is possible that they contain some GFs secreted by hUC-MSCs, and therefore partly contribute to tissue regeneration processes [104].…”

Section: The Acellular Fractionsmentioning

confidence: 99%

“…The hUCS is the non-cellular supernatant obtained when whole hUC blood clots, and is rich in cytokines and GFs with relevant biological properties, such as many ILs, G-CSF, GM-CSF, FGF, NGF, tumor necrosis factor beta, and VEGF, among others [114,115] (Table 1). Compared to hUCP, the hUCS is distinguished by a higher concentration of VEGF, which has been associated with pronounced angiogenic and anti-apoptotic effects, via PI3K/AKT and MAPK signaling pathways.…”

Section: Human Umbilical Cord Serummentioning

confidence: 99%

Human Umbilical Cord-Based Therapeutics: Stem Cells and Blood Derivatives for Female Reproductive Medicine

Rodríguez-Eguren

Gómez-Álvarez²,

Francés-Herrero

et al. 2022

IJMS

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There are several conditions that lead to female infertility, where traditional or conventional treatments have limited efficacy. In these challenging scenarios, stem cell (SC) therapies have been investigated as alternative treatment strategies. Human umbilical cord (hUC) mesenchymal stem cells (hUC-MSC), along with their secreted paracrine factors, extracts, and biomolecules, have emerged as promising therapeutic alternatives in regenerative medicine, due to their remarkable potential to promote anti-inflammatory and regenerative processes more efficiently than other autologous treatments. Similarly, hUC blood derivatives, such as platelet-rich plasma (PRP), or isolated plasma elements, such as growth factors, have also demonstrated potential. This literature review aims to summarize the recent therapeutic advances based on hUC-MSCs, hUC blood, and/or other plasma derivatives (e.g., extracellular vesicles, hUC-PRP, and growth factors) in the context of female reproductive medicine. We present an in-depth analysis of the principal molecules mediating tissue regeneration, compiling the application of these therapies in preclinical and clinical studies, within the context of the human reproductive tract. Despite the recent advances in bioengineering strategies that sustain delivery and amplify the scope of the therapeutic benefits, further clinical trials are required prior to the wide implementation of these alternative therapies in reproductive medicine.

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Human Umbilical Cord Blood Serum/Plasma: Cytokine Profile and Prospective Application in Regenerative Medicine

Cited by 18 publications

References 25 publications

Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism

Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism

Tubulin‐binding peptide RR‐171 derived from human umbilical cord serum displays antitumor activity against hepatocellular carcinoma via inducing apoptosis and activating the NF‐kappa B pathway

Human Umbilical Cord-Based Therapeutics: Stem Cells and Blood Derivatives for Female Reproductive Medicine

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