Human umbilical cord blood cells form epidermis in the skin equivalent model

Mortier, Laurent; Delesalle, F.; Formstecher, Pierre; Polakowska, Renata

doi:10.1111/j.1600-0625.2010.01131.x

Cited by 10 publications

(11 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To this end, various techniques using human DFs are currently being investigated (3-6) as well as other fibroblast populations (7,8), alongside the generation of serum-free medias (3,6).…”

Section: Introductionmentioning

confidence: 99%

Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture

Hill

Gardner

Crawford

et al. 2013

Experimental Dermatology

View full text Add to dashboard Cite

Additional Supporting Information may be found in the online version of this article: Figure S1. Sequence comparison between Foxq1+/+ (top) and Foxq1 (bottom) genes. Note the C to T transition at nucleotide 490 between the control (top) and mutant (bottom) alleles. Abstract: Traditional skin grafting techniques are effective but limited methods of skin replacement. Autologous transplantation of rapidly cultured keratinocytes is successful for epidermal regeneration, but the current gold-standard technique requires mouse fibroblast feeders and serum-rich media, with serum-free systems and dermal fibroblast (DF) feeders performing relatively poorly. Here, we investigated the capacity of human hair follicle dermal cells to act as alternative supports for keratinocyte growth. Dermal papilla (DP) dermal sheath (DS), DF and 3T3 cells were used as inactivated feeder cells for human keratinocyte coculture. Under conditions favouring dermal cells, proliferation of keratinocytes in the presence of either DS or DP cells was significantly enhanced compared with DF cells, at levels comparable to keratinocytes cultured under gold-standard conditions. Secreted protein acidic and rich in cysteine (SPARC) expression increased DS and DP cells relative to DFs; however, further experiments did not demonstrate a role in keratinocyte support.

show abstract

“…To this end, various techniques using human DFs are currently being investigated (3-6) as well as other fibroblast populations (7,8), alongside the generation of serum-free medias (3,6).…”

Section: Introductionmentioning

confidence: 99%

Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture

Hill

Gardner

Crawford

et al. 2013

Experimental Dermatology

View full text Add to dashboard Cite

show abstract

“…and Mortier et al. that stem cells were successfully isolated from umbilical cord blood and could subsequently be differentiated into epithelial cells in vitro . These studies supported the proposal that umbilical cord blood–derived MSCs could be used as a starting material for the expansion of cells needed for the treatment of large skin defects.…”

Section: Umbilical Cord–derived Mesenchymal Stem Cells (Uc‐mscs) and mentioning

confidence: 52%

Application of stems cells in wound healing—An update

Teng

Huang

Zhang

2014

Wound Repair Regeneration

View full text Add to dashboard Cite

Wound healing is a complex but well-orchestrated tissue repair process composed of a series of molecular and cellular events conducted by various types of cells and extracellular matrix. Despite a variety of therapeutic strategies proposed to accelerate the healing of acute and/or chronic wounds over the past few decades, effective treatment of chronic nonhealing wounds still remains a challenge. Due to the recent advances in stem cell research, a dramatic enthusiasm has been drawn to the application of stem cells in regenerative medicine. Both embryonic and adult stem cells have prolonged self-renewal capacity and are able to differentiate into various tissue types. Nevertheless, use of embryonic stem cells is limited, owing to ethical concerns and legal restrictions. Adult stem cells, which could be isolated from bone marrow, umbilical cord blood, adipose tissue, skin and hair follicles,are being explored extensively to facilitate the healing of both acute and chronic wounds. The current article summarizes recent research on various types of stem cell-based strategies applied to improve wound healing. In addition, future directions of stem cell-based therapy in wound healing have also been discussed. Finally, despite its apparent advantages, limitations and challenges of stem cell therapy are discussed.

show abstract

“…Stem cells are required at the site of injury to allow the regeneration of dermal, epidermal and muscle tissue . Moreover, they promote scarless wound healing by generating a regenerative microenvironment via the secretion of protective factors that can inhibit myofibroblasts in a paracrine fashion …”

Section: Stem Cells Repair Wounds and Facilitate Skin And Muscle Regementioning

confidence: 99%

“…75 Moreover, they promote scarless wound healing by generating a regenerative microenvironment via the secretion of protective factors that can inhibit myofibroblasts in a paracrine fashion. [76][77][78][79][80][81] Following skin wounding, epidermal stem cells in the basal layer are activated, proliferate and migrate to the site of injury where they contribute to regeneration. 82 Muscle repair following injury is facilitated by SCs.…”

Section: Stem Cells Repair Wounds and Facilitate Skin And Muscle Rementioning

confidence: 99%

Tissue engineering strategies combining molecular targets against inflammation and fibrosis, and umbilical cord blood stem cells to improve hampered muscle and skin regeneration following cleft repair

Schreurs

Suttorp

Mutsaers

et al. 2019

Medicinal Research Reviews

View full text Add to dashboard Cite

Cleft lip with or without cleft palate is a congenital deformity that occurs in about 1 of 700 newborns, affecting the dentition, bone, skin, muscles and mucosa in the orofacial region. A cleft can give rise to problems with maxillofacial growth, dental development, speech, and eating, and can also cause hearing impairment. Surgical repair of the lip may lead to impaired regeneration of muscle and skin, fibrosis, and scar formation. This may result in hampered facial growth and dental development affecting oral function and lip and nose esthetics. Therefore, secondary surgery to correct the scar is often indicated. We will discuss the molecular and cellular pathways involved in facial and lip myogenesis, muscle anatomy in the normal and cleft lip, and complications following surgery. The aim of this review is to outline a novel molecular and cellular strategy to improve musculature and skin regeneration and to reduce scar formation following cleft repair. Orofacial clefting can be diagnosed in the fetus through prenatal ultrasound screening and allows planning for the harvesting of umbilical cord blood stem cells upon birth. Tissue engineering techniques using these cord blood stem cells and molecular targeting of inflammation and fibrosis during surgery may promote tissue regeneration. We expect that this novel strategy improves both muscle and skin regeneration, resulting in better function and esthetics after cleft repair.

show abstract

Human umbilical cord blood cells form epidermis in the skin equivalent model

Cited by 10 publications

References 20 publications

Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture

Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture

Application of stems cells in wound healing—An update

Tissue engineering strategies combining molecular targets against inflammation and fibrosis, and umbilical cord blood stem cells to improve hampered muscle and skin regeneration following cleft repair

Contact Info

Product

Resources

About