Human Tissue-Resident Stem Cells Combined with Hyaluronic Acid Gel Provide Fibrovascular-Integrated Soft-Tissue Augmentation in a Murine Photoaged Skin Model

Altman, Andrew; Khalek, Feras J. Abdul; Seidensticker, Max; Pinilla, Severin; Yan, Yasheng; Coleman, Micheal; Song, Yanyu; Butler, Charles E.; Alt, Eckhard

doi:10.1097/prs.0b013e3181c2a59a

Cited by 40 publications

(37 citation statements)

References 18 publications

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“…Similar synergistic effects have been seen with the incorporation of different types of cells at the repair site in various wound-healing models [39][40][41] and, in particular, in hernia repairs and laparotomies. 30,49,50 For instance, Xing et al 50 used amnion-derived progenitor cells in laparotomies, and Zhao et al 49 used bone marrow-derived mesenchymal stem cells seeded onto decellularized dermal scaffolds in abdominal wall repairs.…”

Section: Discussionsupporting

confidence: 54%

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Adipose-Derived Stem-Cell-Seeded Non-Cross-Linked Porcine Acellular Dermal Matrix Increases Cellular Infiltration, Vascular Infiltration, and Mechanical Strength of Ventral Hernia Repairs

Iyyanki

Dunne

Zhang

et al. 2015

Tissue Engineering Part A

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Adipose-derived stem cells (ASCs) facilitate wound healing by improving cellular and vascular recruitment to the wound site. Therefore, we investigated whether ASCs would augment a clinically relevant bioprosthetic mesh-non-cross-linked porcine acellular dermal matrix (ncl-PADM)-used for ventral hernia repairs in a syngeneic animal model. ASCs were isolated from the subcutaneous adipose tissue of Brown Norway rats, expanded, and labeled with green fluorescent protein. ASCs were seeded (2.5 · 10 4 cells/cm 2 ) onto ncl-PADM for 24 h before surgery. In vitro ASC adhesion to ncl-PADM was assessed at 0.5, 1, and 2 h after seeding, and cell morphology on ncl-PADM was visualized by scanning electron microscopy. Ventral hernia defects (2 · 4 cm) were created and repaired with ASC-seeded (n = 31) and control (n = 32) ncl-PADM. Explants were harvested at 1, 2, and 4 weeks after surgery. Explant remodeling outcomes were evaluated using gross evaluation (bowel adhesions, surface area, and grade), histological analysis (hematoxylin and eosin and Masson's trichrome staining), immunohistochemical analysis (von Willebrand factor VIII), fluorescent microscopy, and mechanical strength measurement at the tissue-bioprosthetic mesh interface. Stem cell markers CD29, CD90, CD44, and P4HB were highly expressed in cultured ASCs, whereas endothelial and hematopoietic cell markers, such as CD31, CD90, and CD45 had low expression. Approximately 85% of seeded ASCs adhered to ncl-PADM within 2 h after seeding, which was further confirmed by scanning electron microcopy examination. Gross evaluation of the hernia repairs revealed weak omental adhesion in all groups. Ultimate tensile strength was not significantly different in control and treatment groups. Conversely, elastic modulus was significantly greater at 4 weeks postsurgery in the ASC-seeded group ( p < 0.001). Cellular infiltration was significantly higher in the ASC-seeded group at all time points ( p < 0.05). Vascular infiltration was significantly greater at 4 weeks postsurgery in the ASC-seeded group ( p < 0.001). The presence of ASCs improved remodeling outcomes by yielding an increase in cellular infiltration and vascularization of ncl-PADM and enhanced the elastic modulus at the ncl-PADM-tissue interface. With the ease of harvesting adipose tissues that are rich in ASCs, this strategy may be clinically translatable for improving ncl-PADM ventral hernia repair outcomes.

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Section: Discussionsupporting

confidence: 54%

“…The role of ASCs in regenerative wound healing [39][40][41] has been established and is currently being avidly explored. Wound healing occurs in stages that include inflammatory cell infiltration, collagen deposition, vascularization, and remodeling.…”

Section: Discussionmentioning

confidence: 99%

Adipose-Derived Stem-Cell-Seeded Non-Cross-Linked Porcine Acellular Dermal Matrix Increases Cellular Infiltration, Vascular Infiltration, and Mechanical Strength of Ventral Hernia Repairs

Iyyanki

Dunne

Zhang

et al. 2015

Tissue Engineering Part A

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“…They apparently actively incorporate into the hyaluronic acid fill and show an organized fibrovascular network, inducing a significant increase in procollagen 1-alpha 2mRNA expression compared with controls to correct wrinkles or depressions [25]. The essential principle of a scaffold is that it is a biological framework that allows stem cells to attach, grow, and differentiate [26].…”

Section: Discussionmentioning

confidence: 98%

Immediate cell-supplemented lipotransfer (iCSL)

Condé-Green¹,

Lamblet²

2011

Eur J Plast Surg

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Studies have shown that adipose-derived stem cells may improve neovascularization, graft take, and much more. Many strategies have been developed in the last years to isolate and take advantage of these cells for different applications in many specialties. Immediate cellsupplemented lipotransfer (iCSL) is a reproducible and immediate approach that consists in isolating a great number of stem cells from the harvested fat and injecting the suspension with processed adipose tissue as a scaffold in the same procedure, in a short time and cost-effective manner. In this article, the authors present iCSL throughout its processing method, its advantages and current applications in plastic and reconstructive surgery, and its potential applications in all fields of regenerative medicine

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“…Hyaluronic acid is used as a gel matrix scaffold to deliver the growth factor medium, and is known to bind CD44 that is expressed by MSCs. Its ability to bind to CD44 receptors on the stem cells was found to stabilize stem cells; and hyaluronic acid was used as a scaffold and delivery system of adipose derived stem cells in treating wounds as a dermal filler and in adipose tissue regeneration (Altman et al (2010), Chung et al (2010), Cervelli et al (2010) ). Hyaluronic acid itself is approved by the United States Food and Drug Administration as a dermal filler (Beer (2009) (Ramsay et al (1989), Chen et al (1996)).…”

Section: Components Of Growth Factormentioning

confidence: 99%

Novel Regenerative Solutions Induce Rapid Adipogenic Differentiation of Mesenchymal Stem Cells with No Evidence of Transformation or Osteogenic Differentiation

Rodgerson¹,

Harris²,

Giampapa³

et al. 2013

ASC

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Adipose tissue is commonly used in plastic surgery procedures and is also a rich source of mesenchymal stem cells (MSCs). Since MSCs are functionally plastic cells, adipose tissue may provide a promising cell source for regenerative medicine applications. We have developed two mixed growth factor formulations (termed "dermal" and "fat" factor formulations) to be used with autologous adipose tissue to help induce the regeneration of human skin and subcutaneous tissue. In this study, we investigate the effect of these formulations on proliferation and differentiation of MSCs obtained from human bone marrow and from adipose tissues. Lower concentrations of both formulations had no effect on MSC proliferation, but induced rapid adipogenic differentiation. At higher concentrations, both formulations induced MSC death. There was no evidence of osteogenesis ormalignant transformation at any concentration, as judged by absence of foci formation, inability of the cells to be serially passaged and absence of colonies in methylcellulose matrix. Thus, at optimal doses these factor formulations promote rapid differentiation of MSCs into adipogenic cells, with no evidence of cell transformation or osteogenic induction. These studies have implications for wound healing and tissue regeneration, as well as cosmetic applications to enhance the deficits seen in aging skin and in facial volume loss.

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Human Tissue-Resident Stem Cells Combined with Hyaluronic Acid Gel Provide Fibrovascular-Integrated Soft-Tissue Augmentation in a Murine Photoaged Skin Model

Abstract: The combination of adipose tissue-derived stem cells and nonanimal stabilized hyaluronic acid holds promise as a tool with which to achieve lasting volume fill in reconstructive surgical soft-tissue augmentation.

Cited by 40 publications

References 18 publications

Adipose-Derived Stem-Cell-Seeded Non-Cross-Linked Porcine Acellular Dermal Matrix Increases Cellular Infiltration, Vascular Infiltration, and Mechanical Strength of Ventral Hernia Repairs

Adipose-Derived Stem-Cell-Seeded Non-Cross-Linked Porcine Acellular Dermal Matrix Increases Cellular Infiltration, Vascular Infiltration, and Mechanical Strength of Ventral Hernia Repairs

Immediate cell-supplemented lipotransfer (iCSL)

Novel Regenerative Solutions Induce Rapid Adipogenic Differentiation of Mesenchymal Stem Cells with No Evidence of Transformation or Osteogenic Differentiation

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