Human tissue engineering allows the identification of active miRNA regulators of glioblastoma aggressiveness

Glut3 Addiction Is a Druggable Vulnerability for a Molecularly Defined Subpopulation of Glioblastoma

“…Valérie Dutoit and Dr. Pierre-Yves Dietrich provided the Ge269, 479, 518, 688, 738, 835, 885, 898, 904, 970.2 models, derived as previously described (Cosset et al, 2016) from patients with stage IV glioma under approval of the Institutional Review Board from the University of Geneva. GBM6 and GBM39 were gifts from Dr. Paul Mischel and cultured in GSC medium.…”

Glioblastoma Stem Cells: Driving Resilience through Chaos

“…In cancer, recapitulating cancer stem cell niches in homogenous, nutrient-rich in vitro cultures and in animal models is an ongoing challenge. To address this issue, several culture systems have attempted to mimic the heterogeneity of the tumor microenvironment in vitro through 3D culture or microfluidic approaches [77][78][79]. Replacing convenient but simplistic models with more accurate and complex models will be crucial in developing effective therapies.…”

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

“…miR-494 is overexpressed in glioblastomas as compared to normal brain, and is a statistically important miRNA with regard to survival when patients are segregated according to therapy vs. no-therapy (95). Target genes for this miRNA reported in the literature include those involved in cell signaling, metabolism, and apoptosis; increased expression of this miRNA leads to increased cell proliferation and decreased apoptosis of glioblastoma cell lines in vitro (95).…”

“…miR-494 is overexpressed in glioblastomas as compared to normal brain, and is a statistically important miRNA with regard to survival when patients are segregated according to therapy vs. no-therapy (95). Target genes for this miRNA reported in the literature include those involved in cell signaling, metabolism, and apoptosis; increased expression of this miRNA leads to increased cell proliferation and decreased apoptosis of glioblastoma cell lines in vitro (95). miR-494 enhances invasiveness of gliomas via EGFR upregulation, protein kinase B (Akt) activation, and extracellular signal-regulated kinase (ERK) activation, and downregulation of this miRNA in glioblastoma stem-like cells leads to increased apoptosis and suppresses invasion and proliferation (95).…”

“…Target genes for this miRNA reported in the literature include those involved in cell signaling, metabolism, and apoptosis; increased expression of this miRNA leads to increased cell proliferation and decreased apoptosis of glioblastoma cell lines in vitro (95). miR-494 enhances invasiveness of gliomas via EGFR upregulation, protein kinase B (Akt) activation, and extracellular signal-regulated kinase (ERK) activation, and downregulation of this miRNA in glioblastoma stem-like cells leads to increased apoptosis and suppresses invasion and proliferation (95). For this reason, it has been proposed as a therapeutic target in human glioblastoma (96,97) miR-216b: Downregulated in Hypoxia in Canine High-Grade Glioma Cell Lines miR-216b is significantly downregulated in glioma cells and tissues in comparison to normal brain, and ectopic expression of this miRNA inhibits proliferation and invasion in both in vitro and in vivo xenograft models (98).…”