Human thymic stromal lymphopoietin enhances expression of CD80 in human CD14+ monocytes/macrophages

Hirano, Reiji; Hasegawa, Shunji; Hashimoto, Kunio; Haneda, Yasuhiro; Ohsaki, Ayami; Ichiyama, Takashi

doi:10.1007/s00011-011-0310-0

Cited by 18 publications

(13 citation statements)

References 41 publications

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“…The degree of mCD14 down-modulation on monocytes suggests the preferential expansion of a small CD14 low subset and not a globally altered transcription/translation rate. This, for example, is in line with the increased percentage of CD14 −/low monocytes upon in vitro culture in the presence of TSLP, an important cytokine in allergic asthma 40 . Monocytes are heterogeneous, with major (CD14 high ) and minor (CD14 low ) subsets 32 .…”

Section: Discussionsupporting

confidence: 73%

The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes

Nieto‐Fontarigo

Salgado

San-José

et al. 2018

Sci Rep

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LPS-ligation to CD14/TLR-4 on monocytes/macrophages triggers the production of IL-12-family cytokines. IL12/18 promote TH1-differentiation, counteracting the TH2-driven asthma. Therefore, CD14 modulation could alter the TH2-differentiation and should be taken into account when studying asthma. To analyse the alteration in CD14 levels and its association with CD14 (−159 C/T) SNP (rs2569190) in Caucasian adults with stable allergic asthma, we performed a cross-sectional study (277 healthy subjects vs. 277 patients) where clinical parameters, CD14 values and the CD14 (−159 C/T) SNP were studied. Apart from typical biomarkers, we found an increment of neuron-specific enolase (NSE) in allergic asthma, probably linked to monocyte activity. Indeed, we evidenced increased monocyte numbers, but lower CD14 expression and normalised sCD14 values in patients. Moreover, we noticed an association of the T allele (P = 0.0162) and TT genotype (P = 0.0196) of the CD14 SNP with a decreased risk of allergic asthma and augmented sCD14 levels. In conclusion, monocyte CD14 expression and normalized sCD14 values were reduced in stable state asthmatics, and this could be related to the presence of an expanded CD14low monocyte subset. This study also demonstrates that the CD14 (−159 C/T) polymorphism is a risk factor for moderate-severe allergic asthma in adult Caucasians.

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Section: Discussionsupporting

confidence: 73%

The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes

Nieto‐Fontarigo

Salgado

San-José

et al. 2018

Sci Rep

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“…In addition, TSLP can enhance eosinophil survival, up-regulate surface expression of adhesion molecules and induce the release of inflammatory cytokines and chemokines from human eosinophils [ 47 ]. Finally, TSLP amplifies M2 macrophage polarization [ 48 , 49 ]. Since eosinophils and, at a lower extent, basophils/mast cells are important components of the HL lymph node infiltrate, it is conceivable that IL-31 and/or TSLP contribute to the recruitment, survival and activation of these cell types and, more in general, polarize immune responses towards a tumor promoting functional state.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-31 and thymic stromal lymphopoietin expression in plasma and lymph node from Hodgkin lymphoma patients

Ferretti

Hohaus

Napoli³

et al. 2017

Oncotarget

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Hodgkin Lymphoma (HL) is a tumor of B-cell origin characterized by Hodgkin and Reed-Stenberg (H/RS) cells embedded in an inflammatory tissue where numerous cytokines/chemokines contribute to shape the microenvironment, leading to the typical clinical symptoms.We investigated: i) the expression of Interleukin-IL-31 (IL-31) and Thymic Stromal Lymphopoietin (TSLP), two Th2-related cytokines with tumor-promoting and pruritogenic functions, and of the respective receptors in HL invaded lymph nodes by flow cytometry, and ii) the potential association of IL-31/TSLP plasma concentrations with clinical characteristics by ELISA.H/RS cells and the major immune cell types infiltrating HL lymph nodes expressed intracytoplasmic and surface IL-31/TSLP, and their receptors. A subgroup of patients showing at diagnosis elevated IL-31 and TSLP plasma levels had an International Prognostic Score>2, indicative of high risk of relapse, and a subsequent positive interim PET-scan, indicative of insufficient response to chemotherapy. No correlation was found between IL-31/TSLP plasma levels and overall or event-free survival.In conclusion, IL-31/TSLP and their receptors are expressed in HL cells and in immune cells infiltrating affected lymph nodes, where both cytokines may contribute to local immune suppression. The clinical impact of IL-31 and TSLP plasma levels has to be further defined in larger patient cohorts.

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“…Epithelial cells, which highly express TSLP in allergic Th2‐based disorders, such as atopic dermatitis and asthma (18), were originally identified as the principal source of TSLP. More recently, several studies have shown that TSLP can be expressed by other cell types, including fibroblasts, mast cells, CD68+ macrophages (19, 20), and dendritic cells (21). In our study, we observed strong expression of TSLP in the skin of dcSSc patients mainly in immune cells, colocalizing with CD163+ cells, a marker for resident activated macrophages (22), and also colocalizing with a few CD4+ and CD8+ T cells.…”

Section: Discussionmentioning

confidence: 99%

Thymic Stromal Lymphopoietin Is Up‐Regulated in the Skin of Patients With Systemic Sclerosis and Induces Profibrotic Genes and Intracellular Signaling That Overlap With Those Induced by Interleukin‐13 and Transforming Growth Factor β

Christmann¹,

Mathes²,

Affandi³

et al. 2013

Arthritis & Rheumatism

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Objective To explore the expression of thymic stromal lymphopoietin (TSLP) in patients with diffuse cutaneous systemic sclerosis (dcSSc) and compare its effects in vivo and in vitro with those of interleukin-13 (IL-13) and transforming growth factor β (TGFβ). Methods Skin biopsy specimens from patients with dcSSc (n = 14) and healthy controls (n = 13) were analyzed by immunohistochemistry and immunofluorescence for TSLP, TSLP receptor, CD4, CD8, CD31, and CD163 markers. Wild-type, IL-4Rα1–, and TSLP-deficient mice were treated with TGFβ, IL-13, poly(I-C), or TSLP by osmotic pump. Human fibroblasts and peripheral blood mononuclear cells (PBMCs) were stimulated with TGFβ, IL-13, poly(I-C), or TSLP. Microarray analysis and quantitative polymerase chain reaction were performed to determine gene expression, and protein levels of phospho-Smad2 and macrophage marker CD163 were tested. Results TSLP was highly expressed in the skin of dcSSc patients, more strongly in perivascular areas and in immune cells, and was produced mainly by CD163+ cells. The skin of TSLP-treated mice showed up-regulated clusters of gene expression that overlapped strongly with those in IL-13– and TGFβ-treated mice. TSLP up-regulated specific genes, including CXCL9, proteasome, and interferon (IFN)–regulated genes. TSLP treatment in IL-4Rα1–deficient mice promoted similar cutaneous inflammation as in wild-type mice, though TSLP-induced arginase 1, CCL2, and matrix metalloproteinase 12 messenger RNA levels were blocked. In PBMCs, TSLP up-regulated tumor necrosis factor α, Mx-1, IFNγ, CXCL9, and mannose receptor 1 gene expression. TSLP-deficient mice treated with TGFβ showed less fibrosis and blocked expression of plasminogen activator inhibitor 1 and osteopontin 1. Poly(I-C)–treated mice showed high levels of cutaneous TSLP. Conclusion TSLP is highly expressed in the skin of dcSSc patients and interacts in a complex manner with 2 other profibrotic cytokines, TGFβ and IL-13, strongly suggesting that it might promote SSc fibrosis directly or indirectly by synergistically stimulating pro-fibrotic genes, or production of these cytokines.

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Human thymic stromal lymphopoietin enhances expression of CD80 in human CD14+ monocytes/macrophages

Abstract: TSLP induces CD80 expression in human peripheral blood CD14(+) monocytes/macrophages; this indicates monocyte/macrophage activation. This may be associated with their differentiation into myeloid dendritic cells.

Cited by 18 publications

References 41 publications

The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes

The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes

Interleukin-31 and thymic stromal lymphopoietin expression in plasma and lymph node from Hodgkin lymphoma patients

Thymic Stromal Lymphopoietin Is Up‐Regulated in the Skin of Patients With Systemic Sclerosis and Induces Profibrotic Genes and Intracellular Signaling That Overlap With Those Induced by Interleukin‐13 and Transforming Growth Factor β

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