Human textiles: A cell-synthesized yarn as a truly “bio” material for tissue engineering applications

Magnan, Laure; Labrunie, Gaëlle; Fénelon, Mathilde; Dusserre, Nathalie; Foulc, Marie-Pierre; Lafourcade, Mickaël; Svahn, Isabelle; Gontier, Étienne; Vélez, H V Jaime; McAllister, Todd; L’Heureux, Nicolas

doi:10.1016/j.actbio.2020.01.037

Cited by 40 publications

(58 citation statements)

References 46 publications

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“…Initially, the devitalised grafts were still partially re-vitalised by in vitro lining with the patient's own endothelial cells ( 293 ). This hybrid approach was followed by completely devitalised non-endothelialised allogenic grafts (“Lifeline Grafts®”) ( 294 ) eventually leading to woven textile structures using yarns of devitalised in vitro grown tissues ( 333 ). No repopulation, however, was ever reported in any of these grafts.…”

Section: A Protracted Evolutionmentioning

confidence: 99%

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Zilla

Deutsch

Bezuidenhout

et al. 2020

Front. Cardiovasc. Med.

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Section: A Protracted Evolutionmentioning

confidence: 99%

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Zilla

Deutsch

Bezuidenhout

et al. 2020

Front. Cardiovasc. Med.

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“…Red arrows show the direction of the force generated by tissue tension. (a) Human cells grew on and CAM accumulated near the wire slowly enveloping it, meanwhile, the CAM detached from the wall and folded over the wire enveloping it (steps [3] and [4]). (b) Phase-contrast micrograph showing folding over the anchor (at 3 weeks).…”

Section: Discussionmentioning

confidence: 99%

“…(d) Ovine cells were more contractile and developed earlier/larger tissue tension pulling away the CAM accumulating against the wire before it could envelop it (step [3]). Without a strong attachment, the sheets slipped under the wire and contracted (step [4]). (e) Phase-contrast micrograph of an ovine sheet showing tissue separation from the anchor and a lack of folding after 1 week.…”

Section: Discussionmentioning

confidence: 99%

“…Also, human sheets could detach from the wall of the well due to the tissue tension and fall on the wire surrounding it (Figure 8(a) step [3] and (b)). After 3 weeks, both events prevented human sheet detachment for their entire culture period (Figure 8(a) step [4] and (c)). However, OSFs were more contractile and pulled more than HSFs on the sheet, which led to the sheet sliding under the wire after a week (Figure 8(d) step [3] and (e)) and the ovine sheet detachment a week later (Figure 8(d) step [4] and (f)).…”

Section: Pla Anchors Avoid Ovine Cam Sheet Loss Due To Detachmentmentioning

confidence: 99%

“…After 3 weeks, both events prevented human sheet detachment for their entire culture period (Figure 8(a) step [4] and (c)). However, OSFs were more contractile and pulled more than HSFs on the sheet, which led to the sheet sliding under the wire after a week (Figure 8(d) step [3] and (e)) and the ovine sheet detachment a week later (Figure 8(d) step [4] and (f)). We hypothesized that a surface allowing for better cell adhesion would lead to a faster enveloping of the wire, hence we replaced the stainless-steel wire by a PLA wire (Figure 8(g) and (h)).…”

Section: Pla Anchors Avoid Ovine Cam Sheet Loss Due To Detachmentmentioning

confidence: 99%

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Cell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells

et al. 2021

Self Cite

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We have created entirely biological tissue-engineered vascular grafts (TEVGs) using sheets of cell-assembled extracellular matrix (CAM) produced by human fibroblasts in vitro. A large animal TEVG would allow long-term pre-clinical studies in a clinically relevant setting (graft size and allogeneic setting). Therefore, canine, porcine, ovine, and human skin fibroblasts were compared for their ability to form CAM sheets. Serum sourcing greatly influenced CAM production in a species-dependent manner. Ovine cells produced the most homogenous and strongest animal CAM sheets but remained ≈3-fold weaker than human sheets despite variations of serum, ascorbate, insulin, or growth factor supplementations. Key differences in cell growth dynamics, tissue development, and tissue architecture and composition were observed between human and ovine. This study demonstrates critical species-to-species differences in fibroblast behavior and how they pose a challenge when attempting to substitute animal cells for human cells during the development of tissue-engineered constructs that require long-term cultures.

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Macro, Micro, and Everything in Between. Bridging the Gap: A Vision Toward the Creation of Multiscale Vascular Networks

Pirraco

2023

Advanced Biology

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Vascularization is a key issue for the clinical translation of tissue engineering strategies. This has been recognized in the field for almost two decades. Several strategies to solve this issue are proposed but none has decisively tackled the problem. This is in part due to an excessive focus on microvascularization that ignores the need of having macrovessels capable of being surgically connected to the patient's circulation upon implantation. Indeed, a strategy for macrovessel engineering must co‐exist with a strategy for microvessels. And if this is true, all the intermediate scales have to be addressed as well. Therefore, multiscale vascular networks must be the focus of tissue engineering vascularization efforts. In this work, a reflection is made on a possible path forward for researchers and engineers in the field to achieve the ultimate goal of efficient vascularization of engineered tissues and organs.

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Human textiles: A cell-synthesized yarn as a truly “bio” material for tissue engineering applications

Cited by 40 publications

References 46 publications

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Cell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells

Macro, Micro, and Everything in Between. Bridging the Gap: A Vision Toward the Creation of Multiscale Vascular Networks

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