Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma

Shen, Congcong; Kang, Yu-Huan; Lin, Yunfeng; Cui, Dandan; He, Yi; Yang, Jinliang; Gou, Lantu

doi:10.1186/0717-6287-47-52

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…Our previous study showed a decreased expression of TEX101 in serum samples of patients who later developed TGCT, compared with controls ( 5 ). Investigating TEX101 expression in seminoma TGCT tissues demonstrated a similar finding, revealing no expression of TEX101, in contrast to normal testis ( 50 ). They hypothesised that the role of TEX101 in cancer progression is due to its possible effect in reducing the function of uPAR, MMPs and cathepsin B.…”

Section: Resultsmentioning

confidence: 55%

“…They hypothesised that the role of TEX101 in cancer progression is due to its possible effect in reducing the function of uPAR, MMPs and cathepsin B. Loss of TEX101 and therefore gain of function of these enzymes could lead to increased cell proliferation and migration of cancer cells in head and neck squamous cell carcinoma as well as in TGCT ( 47 , 50 ).…”

Section: Resultsmentioning

confidence: 99%

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A Role of the TEX101 Interactome in the Common Aetiology Behind Male Subfertility and Testicular Germ Cell Tumor

et al. 2022

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Patients who develop testicular germ cell tumours (TGCT) are at higher risk to be subfertile than the general population. The conditions are believed to originate during foetal life, however, the mechanisms behind a common aetiology of TGCT and male subfertility remains unknown. Testis-expressed 101 (TEX101) is a glycoprotein that is related to male fertility, and downregulation of the TEX101 gene was shown in pre-diagnostic TGCT patients. In this review, we summarize the current knowledge of TEX101 and its interactome related to fertility and TGCT development. We searched literature and compilation of data from curated databases. There are studies from both human and animals showing that disruption of TEX101 result in abnormal semen parameters and sperm function. Members of the TEX101 interactome, like SPATA19, Ly6k, PICK1, and ODF genes are important for normal sperm function. We found only two studies of TEX101 related to TGCT, however, several genes in its interactome may be associated with TGCT development, such as PLAUR, PRSS21, CD109, and ALP1. Some of the interactome members are related to both fertility and cancer. Of special interest is the presence of the glycosylphosphatidylinositol anchored proteins TEX101 and PRSS21 in basophils that may be coupled to the immune response preventing further development of TGCT precursor cells. The findings of this review indicate that members of the TEX101 interactome could be a part of the link between TGCT and male subfertility.

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Section: Resultsmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 99%

A Role of the TEX101 Interactome in the Common Aetiology Behind Male Subfertility and Testicular Germ Cell Tumor

et al. 2022

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“…Top2a [ 45 ], Scml2 [ 46 ], and many others) and, in some cases, even during spermiogenesis (Additional file 2 : Dataset S1). Examples of the latter are the genes coding for sperm surface-heat shock protein HSPA5 [ 47 ], TEX101 [ 48 ] (for the latter two see also Fig. 4c ) and its interacting proteins DPEP3 [ 49 ] and LY6K [ 50 ], to name a few.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage

et al. 2016

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BackgroundSpermatogenesis is a complex differentiation process that involves the successive and simultaneous execution of three different gene expression programs: mitotic proliferation of spermatogonia, meiosis, and spermiogenesis. Testicular cell heterogeneity has hindered its molecular analyses. Moreover, the characterization of short, poorly represented cell stages such as initial meiotic prophase ones (leptotene and zygotene) has remained elusive, despite their crucial importance for understanding the fundamentals of meiosis.ResultsWe have developed a flow cytometry-based approach for obtaining highly pure stage-specific spermatogenic cell populations, including early meiotic prophase. Here we combined this methodology with next generation sequencing, which enabled the analysis of meiotic and postmeiotic gene expression signatures in mouse with unprecedented reliability. Interestingly, we found that a considerable number of genes involved in early as well as late meiotic processes are already on at early meiotic prophase, with a high proportion of them being expressed only for the short time lapse of lepto-zygotene stages. Besides, we observed a massive change in gene expression patterns during medium meiotic prophase (pachytene) when mostly genes related to spermiogenesis and sperm function are already turned on. This indicates that the transcriptional switch from meiosis to post-meiosis takes place very early, during meiotic prophase, thus disclosing a higher incidence of post-transcriptional regulation in spermatogenesis than previously reported. Moreover, we found that a good proportion of the differential gene expression in spermiogenesis corresponds to up-regulation of genes whose expression starts earlier, at pachytene stage; this includes transition protein-and protamine-coding genes, which have long been claimed to switch on during spermiogenesis. In addition, our results afford new insights concerning X chromosome meiotic inactivation and reactivation.ConclusionsThis work provides for the first time an overview of the time course for the massive onset and turning off of the meiotic and spermiogenic genetic programs. Importantly, our data represent a highly reliable information set about gene expression in pure testicular cell populations including early meiotic prophase, for further data mining towards the elucidation of the molecular bases of male reproduction in mammals.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2618-1) contains supplementary material, which is available to authorized users.

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“…These proteins play crucial roles in immune responses and cancer progression. 62 S100A8, a key regulator of inflammation and immune response, affects chemotaxis and inflammatory cell activation. It facilitates the aggregation of immune cells at inflammatory sites, intensifying and prolonging the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

scRNA-Seq and Bulk-Seq Analysis Identifies S100A9 Plasma Cells as a Potentially Effective Immunotherapeutic Agent for Multiple Myeloma

Long,

Li,

Tang

et al. 2024

JIR

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Immunological regimens are an important area of research for treating multiple myeloma (MM). Plasma cells play a crucial role in immunotherapy. Patients and Methods: In our study, we used both single-cell RNA sequencing (scRNA-seq) and bulk sequencing techniques to analyze MM patients. We analyzed each sample using gene set variation analysis (GSVA) based on immune-related gene sets. We also conducted further analyses to compare immune infiltration, clinical characteristics, and expression of immune checkpoint molecules between the H-S100A9 and L-S100A9 groups of MM patients. Results: We identified eight subpopulations of plasma cells, with S100A9 plasma cells being more abundant in patients with 1q21 gain and 1q21 diploid. CellChat analysis revealed that GAS and HGF signaling pathways were prominent in intercellular communication of S100A9 plasma cells. We identified 14 immune-related genes in the S100A9 plasma cell population, which allowed us to classify patients into the H-S100A9 group or the L-S100A9 group. The H-S100A9 group showed higher ESTIMATE, immune and stroma scores, lower tumor purity, and greater immune checkpoint expression. Patients with 1q21 gain and four or more copies had the lowest ESTIMATE score, immune score, stroma score, and highest tumor purity. Drug sensitivity analysis indicated that the H-S100A9 group had lower IC50 values and greater drug sensitivity compared to the L-S100A9 group. Quantitative reverse transcription (RT-q) PCR showed significantly elevated expression of RNASE6, LYZ, S100A8, S100A9, and S100A12 in MM patients compared to the healthy control group. Conclusion:Our study has identified a correlation between molecular subtypes of S100A9 plasma cells and the response to immunotherapy in MM patients. These findings improve our understanding of tumor immunology and provide guidance for developing effective immunotherapy strategies for this patient population.

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Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma

Cited by 6 publications

References 22 publications

A Role of the TEX101 Interactome in the Common Aetiology Behind Male Subfertility and Testicular Germ Cell Tumor

A Role of the TEX101 Interactome in the Common Aetiology Behind Male Subfertility and Testicular Germ Cell Tumor

Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage

scRNA-Seq and Bulk-Seq Analysis Identifies S100A9 Plasma Cells as a Potentially Effective Immunotherapeutic Agent for Multiple Myeloma

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