Human Tendon–Derived Collagen Hydrogel Significantly Improves Biomechanical Properties of the Tendon-Bone Interface in a Chronic Rotator Cuff Injury Model

Kaizawa, Yukitoshi; Leyden, Jacinta; Behn, Anthony W.; Tulu, U.S.; Franklin, Austin; Wang, Zhen; Abrams, Geoffrey D.; Chang, James; Fox, Paige M.

doi:10.1016/j.jhsa.2018.11.021

Cited by 29 publications

(35 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the prior study, the RTC healing was compared between control and tHG groups. The ultimate load to failure was 19.0 ± 4.5 N in control and 24.2 ± 8.0 N in tHG group at 8 weeks after repair . For the current study, an increase in strength of 20% would be considered clinically significant .…”

Section: Methodsmentioning

confidence: 88%

“…Our results showed that tHG injection improved TBI healing of the SSp tendon biomechanically and increased the fibrocartilage area at the TBI. However, the bone morphometry at the SSp insertion area of the humerus was not significantly improved and restoration of a native TBI structure with four transition zones was not observed histologically …”

Section: Summary Of Recent Reports On Adipose‐derived Stem Cell Implamentioning

confidence: 92%

“…After micro‐CT, samples were decalcified in Immunocal (Decal, Congers, NY) for 48 h. Paraffin embedded 5‐μm‐thick coronal sections of the mid portion of the SSp TBI were stained with Safranin O and Picrosirius Red stains. The areas of metachromasia at the insertion site on Safranin O stained slides were manually outlined and measured using ImageJ software (National Institutes of Health, Bethesda, MD) according to a previously reported method . Collagen fiber organization was assessed based on birefringence under polarized microscopy on the Picrosirius red stained slides as detailed by a previously published method .…”

Section: Methodsmentioning

confidence: 99%

“…Eight weeks after Surgery 1, bilateral SSp tendons were repaired under anesthesia as previously reported. 6 Briefly, the SSp tendon was exposed and dissected free from the LHBT and ISp tendons. Bridging scar tissue between humerus and SSp distal tendon stump was identified.…”

Section: Repair Surgery For Chronic Rotator Cuff Injuries (Surgery 2)mentioning

confidence: 99%

“…However, the bone morphometry at the SSp insertion area of the humerus was not significantly improved and restoration of a native TBI structure with four transition zones was not observed histologically. 6 Scaffolds, cells, and growth factors are three essential factors for tissue engineering. Injection of tHG provides a scaffold for TBI regeneration at the injury site.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Kaizawa

Franklin

Leyden

et al. 2019

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

Rotator cuff (RTC) repair outcomes are unsatisfactory due to the poor healing capacity of the tendon bone interface (TBI). In our preceding study, tendon hydrogel (tHG), which is a type I collagen rich gel derived from human tendons, improved biomechanical properties of the TBI in a rat chronic RTC injury model. Here we investigated whether adipose‐derived stem cell (ASC)‐seeded tHG injection at the repair site would further improve RTC healing. Rats underwent bilateral supraspinatus tendon detachment. Eight weeks later injured supraspinatus tendons were repaired with one of four treatments. In the control group, standard transosseous suture repair was performed. In the ASC, tHG, tHGASC groups, ASC in media, tHG, and ASC‐seeded tHG were injected at repair site after transosseous suture repair, respectively. Eight weeks after repair, the TBI was evaluated biomechanically, histologically, and via micro CT. Implanted ASCs were detected in ASC and tHGASC groups 7 weeks after implantation. ACS implantation improved bone morphometry at the supraspinatus insertion on the humerus. Injection of tHG improved biomechanical properties of the repaired TBI. RTC healing in tHGASC group was significantly better than control but statistically equivalent to the tHG group based on biomechanical properties, fibrocartilage area at the TBI, and bone morphometry at the supraspinatus insertion. In a rat RTC chronic injury model, no biomechanical advantage was gained with ASC augmentation of tHG. Clinical Significance: Tendon hydrogel augmentation with adipose derived stem cells does not significantly improve TBI healing over tHG alone in a chronic rotator cuff injury model. © 2019 Orthopaedic Research Society. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

show abstract

Section: Methodsmentioning

confidence: 88%

Section: Summary Of Recent Reports On Adipose‐derived Stem Cell Implamentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Repair Surgery For Chronic Rotator Cuff Injuries (Surgery 2)mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Kaizawa

Franklin

Leyden

et al. 2019

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

show abstract

Modulation of animal and plant tissue growth with collagen‐starch‐organic molybdenum networks hydrogel biomatrices

Valdés‐Lozano,

Claudio‐Rizo,

Cabrera‐Munguía

et al. 2024

Polymers for Advanced Techs

View full text Add to dashboard Cite

The development of hydrogel biomatrices with potential to modulate animal and plant tissue growth is ongoing. In this study, molybdenum bio‐metal–organic frameworks (MOFs) (Mo‐bioMOFs) incorporating essential amino acids such as l‐histidine (Mo‐His), l‐phenylalanine (Mo‐Phe), and l‐tryptophan (Mo‐Trp) were encapsulated in semi‐interpenetrating polymer network (semi‐IPN) hydrogels composed of collagen and starch. The structure and properties of these materials show dependence on the amino acid that constitutes the Mo‐bioMOFs. The biomatrices have a semi‐crystalline surface with increased porosity when using Mo‐His; this system also benefits swelling. Increased crosslinking, acceleration in gelation, and mechanical improvement are observed for the system based on Mo‐Phe. Methylene blue release experiments were conducted, demonstrating that matrices including Mo‐bioMOFs exhibit controlled release profiles, indicating highly stable retention of Mo‐bioMOFs in the semi‐IPN matrix. The biomatrices enhance the metabolism and proliferation of fibroblasts and monocytes, with Mo‐Trp reducing the secretion of inflammatory cytokines like TNF‐α. The biomatrices exhibit gradual and slow mass loss when exposed to collagenase and commercial vegetable substrates. Both leaf and root cells of tomato plants (Solanum lycopersicum) show increased metabolism and growth when exposed to Mo‐Phe and Mo‐His. Notably, the biomatrix containing Mo‐Phe promotes the most substantial plant growth and foliage after 30 days. These biomatrices have potential applications in chronic wound healing and agriculture.

show abstract

Injectable hydrogels for tendon and ligament tissue engineering

Liu

Zhang

Chen

2020

J Tissue Eng Regen Med

View full text Add to dashboard Cite

The problem of tendon and ligament (T/L) regeneration in musculoskeletal diseases has long constituted a major challenge. In situ injection of formable biodegradable hydrogels, however, has been demonstrated to treat T/L injury and reduce patient suffering in a minimally invasive manner. An injectable hydrogel is more suitable than other biological materials due to the special physiological structure of T/L. Most other materials utilized to repair T/L are cell‐based, growth factor‐based materials, with few material properties. In addition, the mechanical property of the gel cannot reach the normal T/L level. This review summarizes advances in natural and synthetic polymeric injectable hydrogels for tissue engineering in T/L and presents prospects for injectable and biodegradable hydrogels for its treatment. In future T/L applications, it is necessary develop an injectable hydrogel with mechanics, tissue damage‐specific binding, and disease response. Simultaneously, the advantages of various biological materials must be combined in order to achieve personalized precision therapy.

show abstract

Human Tendon–Derived Collagen Hydrogel Significantly Improves Biomechanical Properties of the Tendon-Bone Interface in a Chronic Rotator Cuff Injury Model

Cited by 29 publications

References 40 publications

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Modulation of animal and plant tissue growth with collagen‐starch‐organic molybdenum networks hydrogel biomatrices

Injectable hydrogels for tendon and ligament tissue engineering

Contact Info

Product

Resources

About