Human T lymphocytes become glucocorticoid-sensitive upon immune activation

Galili, Naomi; Galili, Uri; Klein, Eva; Rosenthal, L; Nordenskjöld, Bo

doi:10.1016/0008-8749(80)90297-x

Cited by 40 publications

(17 citation statements)

References 16 publications

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“…After 48 h of culture, the sensitivity of each cell population to GICD was analysed by 7‐aminoactinomycin D (7AAD) staining and analysis by flow cytometry (Figure 1a). Consistent with previous studies 33 , 34 at 48 h, whole PBMCs were completely resistant to DEX treatment; percentage viability by trypan blue exclusion was 90±1% for both VEH and DEX cultures. In line with a previous study of GICD sensitivity in mice, 17 the ratio of viable cell recovery for each lymphocyte population from DEX versus VEH cultures was then used to obtain a quantitative value, termed the survival index (SI), indicating sensitivity to GICD (Figure 1b).…”

Section: Resultssupporting

confidence: 91%

“…Although resting peripheral blood T cells are known to be resistant to GICD, the relative GICD sensitivity of other primary peripheral blood mononucleated cell (PBMC) subsets is currently unknown. [33][34][35][36] To address this, we cultured PBMCs in vitro for 48 h with 10 À7 M dexamethasone (DEX), a concentration corresponding to pharmacological/psychological stress levels in vivo. 37 To avoid changes in gene expression associated with storage of blood at room temperature, PBMCs were extracted from freshly taken blood from a cohort of healthy human donors.…”

Section: Peripheral B Cells Are Highly Susceptible To Gicd In Vitromentioning

confidence: 99%

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The glucocorticoid receptor 1A3 promoter correlates with high sensitivity to glucocorticoid‐induced apoptosis in human lymphocytes

et al. 2014

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Glucocorticoids (GCs) are powerful inhibitors of inflammation and immunity. Although glucocorticoid-induced cell death (GICD) is an important part of GCs actions, the cell types and molecular mechanisms involved are not well understood. Untranslated exon 1A3 of the human glucocorticoid receptor (GR) gene is a major determinant of GICD in GICD-sensitive human cancer cell lines, operating to dynamically upregulate GR levels in response to GCs. We measured the GICD sensitivity of freshly isolated peripheral blood mononuclear cells and thymocytes to dexamethasone in vitro, relating this to GR exon 1A3 expression. A clear GICD sensitivity hierarchy was detected: B cells4thymocytes/natural killer (NK) cells4peripheral T cells. Within thymocyte populations, GICD sensitivity decreased with maturation. Interestingly, NK cell subsets were differentially sensitive to GICD, with CD16 þ CD56 int (cytotoxic) NK cells being highly resistant to GICD, whereas CD16 À CD56 hi (cytokine producing) NK cells were highly sensitive (similar to B cells). B-cell GICD was rescued by co-culture with interleukin-4. Strikingly, although no significant increases in GR protein were observed during 48 h of culture of GICD-sensitive and -resistant cells alike, GR 1A3 expression was increased over pre-culture levels in a manner directly proportional to the GICD sensitivity of each cell type. Accordingly, this is the first evidence that the GR exon 1A3 promoter is differentially regulated during thymic development and maturation of human T cells. Furthermore, human peripheral blood B cells are exquisitely GICD-sensitive in vitro, giving new insight into how GCs may downregulate immunity. Collectively, these data show that GR 1A3 expression is tied with GICD sensitivity in human lymphocytes, underscoring the potential for GR 1A3 expression to be used as a biomarker for sensitivity to GICD.

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Section: Resultssupporting

confidence: 91%

Section: Peripheral B Cells Are Highly Susceptible To Gicd In Vitromentioning

confidence: 99%

The glucocorticoid receptor 1A3 promoter correlates with high sensitivity to glucocorticoid‐induced apoptosis in human lymphocytes

et al. 2014

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“…We then linked the dendrograms obtained using the “dendextend” package (Galili, 2014) to produce a “tanglegram” that allowed us to test the degree of congruence between clustering analyses for each year through a set of metrics characterizing the quality of the dendrogram alignments and the occurrence of nested clusters. We first computed the entanglement index to characterize the quality of the alignment of the two trees in the “tanglegram” layout.…”

Section: Data Analysesmentioning

confidence: 99%

Taxonomic and functional trait diversity of wild bees in different urban settings

Normandin

Vereecken

Buddle

et al. 2017

PeerJ

120

114

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Urbanization is one of the major anthropogenic processes contributing to local habitat loss and extirpation of numerous species, including wild bees, the most widespread pollinators. Little is known about the mechanisms through which urbanization impacts wild bee communities, or the types of urban green spaces that best promote their conservation in cities. The main objective of this study was to describe and compare wild bee community diversity, structure, and dynamics in two Canadian cities, Montreal and Quebec City. A second objective was to compare functional trait diversity among three habitat types (cemeteries, community gardens and urban parks) within each city. Bees were collected using pan traps and netting on the same 46 sites, multiple times, over the active season in 2012 and 2013. A total of 32,237 specimens were identified, representing 200 species and 6 families, including two new continental records, Hylaeus communis Nylander (1852) and Anthidium florentinum (Fabricius, 1775). Despite high community evenness, we found significant abundance of diverse species, including exotic ones. Spatio-temporal analysis showed higher stability in the most urbanized city (Montreal) but low nestedness of species assemblages among the three urban habitats in both cities. Our study demonstrates that cities are home to diverse communities of wild bees, but in turn affect bee community structure and dynamics. We also found that community gardens harbour high levels of functional trait diversity. Urban agriculture therefore contributes substantially to the provision of functionally diverse bee communities and possibly to urban pollination services.

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“…Analysis ofthe T lymphocytes in synovial fluid from patients with rheumatoid arthritis indicates that these cells are activated in terms of proliferation, stable E-rosetteforming capacity, and macrophage-activating activity (21). In view of the ability of LAF (IL 1) to stimulate T-cell proliferation and the generation of stable E-rosette-forming T cells (22), it is possible that macrophages in the synovium through their elaboration of a single factor LAF (IL 1) may regulate the inflammatory activity of both T lymphocytes and synovial cells.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1).

Mizel¹,

Dayer²,

Krane³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

524

194

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Human macrophages produce in culture a factor termed mononuclear cell factor (MCF) that increases the production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. A factor with similar biologic activity is also produced by the murine macrophage cell line P388D1. By using a sequential purification scheme involving ammonium sulfate fractionation; chromatography on DEAE-cellulose, Sephacryl S-200, and phenyl-Sepharose; and discontinuous polyacrylamide gel electrophoresis, the P388D1 cell-derived, synovial cell-stimulating factor was -copurified with the lymphocyte-activating factor [LAF; interleukin 1 (IL 1)]. The specific activity of the partially purified LAF (IL 1) was approximately 15,000-fold higher than that of the LAF (IL 1) in the original P388D1 cell culture supernatant. On the basis of (i) the copurification of the P388D1 cell-derived LAF (IL 1) and synovial cell-stimulating factors; (ii) the similarity in cell of origin, molecular weight, and phenylglyoxal sensitivity of human MCF and murine LAF (IL 1); and (iii)

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Human T lymphocytes become glucocorticoid-sensitive upon immune activation

Cited by 40 publications

References 16 publications

The glucocorticoid receptor 1A3 promoter correlates with high sensitivity to glucocorticoid‐induced apoptosis in human lymphocytes

The glucocorticoid receptor 1A3 promoter correlates with high sensitivity to glucocorticoid‐induced apoptosis in human lymphocytes

Taxonomic and functional trait diversity of wild bees in different urban settings

Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1).

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