Human T-Cell Lymphotropic Virus Type I-Infected Cells Extravasate through the Endothelial Barrier by a Local Angiogenesis-Like Mechanism

Bazarbachi, Ali; Merhi, Raghida Abou; Gessain, Antoine; Talhouk, Rabih; El-Khoury, Hilda; Nasr, Rihab; Gout, Olivier; Sulahian, Rita; Homaidan, Fadia R.; Hermine, Olivier; El-Sabban, Marwan

doi:10.1158/0008-5472.can-03-2390

Cited by 49 publications

(39 citation statements)

References 41 publications

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“…The first step of invasion as well as metastasis is loss of cell-to-cell adhesion (6)(7)(8)(10)(11)(12). Cell-to-cell adhesion is strongly regulated by apparatuses, such as tight junctions, adherens junctions, desmosomes, and gap junctions (14)(15)(16)(17)(18)(19). Decreased expression of the component molecules of these adhesion apparatuses means a decrease of cell-to-cell adhesion, which would result in an increase of invasion activity of the cells.…”

Section: Discussionmentioning

confidence: 99%

“…Cell-to-cell adhesion apparatuses, such as tight junctions, adherens junctions, desmosomes, and gap junction, strongly regulate the invasion activity of cancer cells (14)(15)(16)(17)(18)(19). Tight junctions, one of the epithelial junctional complexes, are located at the most apical part of the complexes and consist of claudin and occludin.…”

Section: Introductionmentioning

confidence: 99%

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Tight Junction Protein Claudin-1 Enhances the Invasive Activity of Oral Squamous Cell Carcinoma Cells by Promoting Cleavage of Laminin-5 γ2 Chain via Matrix Metalloproteinase (MMP)-2 and Membrane-Type MMP-1

et al. 2006

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Although adherent junctions have been extensively studied, the role of tight junctions in cancer cell invasion is not sufficiently explored. We investigated whether claudin-1, a component of tight junctions, regulated invasion activity in oral squamous cell carcinoma (OSC) cells. The expression of claudin-1, activity of matrix metalloproteinase (MMP)-2, and cleavage of laminin-5 ;2 chains were assessed by Western blot analysis, immunohistochemistry, and zymography in OSC cell lines (OSC-4 and NOS-2, highly invasive; OSC-7, weakly invasive) and their xenografts in severe combined immunodeficient (SCID) mice. The influence of claudin-1 small interfering RNA (siRNA) on the invasion activity of the cell lines was also investigated. Compared with OSC-7, both OSC-4 and NOS-2 more strongly expressed claudin-1 and possessed high activities of MMP-2 and MMP-9. Tumors formed in the tongues of SCID mice xenografted with OSC-4, NOS-2, and OSC-7 immunohistochemically revealed strong, moderate, and weak expression of laminin-5 ;2 chains, respectively, and laminin-5 ;2 chains were secreted in the conditioned medium of the cancer cells in parallel with the in vivo results. Claudin-1 siRNA largely suppressed the invasion of OSC-4 and decreased the activation of MMP-2, the expression of membrane-type MMP-1 (MT1-MMP), and the cleavage of laminin-5 ;2. In addition, not only antibodies against MT1-MMP and epidermal growth factor receptor (EGFR) but also MMP-2 and EGFR inhibitors strongly suppressed the invasion activity of OSC-4. These results suggest that claudin-1 upregulates cancer cell invasion activity through activation of MT1-MMP and MMP-2, which results in enhanced cleavage of laminin-5 ;2 chains. (Cancer Res 2006; 66(10): 5251-7)

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tight Junction Protein Claudin-1 Enhances the Invasive Activity of Oral Squamous Cell Carcinoma Cells by Promoting Cleavage of Laminin-5 γ2 Chain via Matrix Metalloproteinase (MMP)-2 and Membrane-Type MMP-1

et al. 2006

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“…Aggressive ATL can be associated with the frequent involvement of the gastrointestinal tract as well as meningeal or cerebral infiltration by neoplastic lymphocytes. The extravasation of ATL cells from the blood stream to these secondary organs is mediated by the expression of matrix metalloproteinase (MMP) -2 and -9 that will, subsequently, degrade the subendothelial basement membrane, hence aiding metastasis (3). In fact, these extracellular proteases have been reported to promote the proliferation, angiogenesis and metastatic potential of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes

Harakeh

Khouzam

Damanhouri

et al. 2014

International Journal of Oncology

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Abstract.Experimental and clinical studies have revealed the effectiveness of a specific nutrient synergy (SNS) mixture composed of ascorbic acid (AA), lysine, proline, arginine, epigallocatechin gallate (EGCG) and other micronutrients in targeting crucial physiological mechanisms involved in cancer progression and metastasis. HTLV-1 causes adult T-cell leukemia (ATL). The spread and metastases of ATL as well as other tumors has been associated with matrix metalloproteinases, especially the gelatinases MMP-2 and MMP-9. The objective of this study was to investigate whether SNS, AA and EGCG affects the gelatinolytic activity of MMP-2 and its transcriptional and translational levels in HTLV-1-positive and -negative malignant T-cells. The results indicated that SNS and EGCG caused a dose-dependent decline in the activity, transcription and translation of MMP-2 after treatment with SNS and EGCG, while AA was only able to inhibit the activity at maximum doses tested and to some extent, the protein expression levels of MMP-2, without affecting their transcriptional levels. The highest activity was noted in the case of SNS which is likely to be due to a synergistic effect of the different constituents in the formulation. These results point towards the potential integration of SNS in the anti-invasive treatment of ATL and related diseases.

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“…1,3,8,14,15 Previous studies have shown possible roles of matrix metalloproteases (MMPs), growth factors and cell adhesion molecules in enhanced tissue infiltration of ATL. [16][17][18][19] Chemokines are a family of structurally related cytokines that induce directed migration of various leukocyte populations through interactions with a group of 7 transmembrane G protein-coupled receptors. 20 It is now known that migration and tissue microenvironmental localization of lymphocytes are finely regulated by the expression of certain cell adhesion molecules and chemokine receptors.…”

mentioning

confidence: 99%

Expression of CCR9 in HTLV‐1⁺ T cells and ATL cells expressing Tax

Nagakubo¹,

Jin²,

Hieshima³

et al. 2007

Intl Journal of Cancer

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Adult T‐cell leukemia (ATL) is a highly aggressive mature CD4+ T‐cell malignancy that is etiologically associated with human T‐lymphotropic virus Type 1 (HTLV‐1). ATL is characterized by frequent infiltration of lymph nodes, spleen, liver, skin and gut. Previously, we and others have shown that the majority of ATL cases are strongly positive for CCR4, which may explain the frequent skin invasion of ATL. Here, we examined whether ATL cells express CCR9, which is involved in T‐cell homing to the gastrointestinal tract. Human T cell lines carrying HTLV‐1 consistently expressed CCR9 together with the HTLV‐1‐encoded transcriptional activator Tax. Although ATL cells freshly isolated from peripheral blood hardly expressed CCR9, ATL cells cultured for 1 day consistently expressed CCR9 in parallel with the upregulation of Tax. Induction of Tax by Cd2+ in JPX‐9, a subline of Jurkat human T cell line carrying Tax under the control of metallothionein promoter, led to upregulation of CCR9. A luciferase reporter gene under the control of the CCR9 promoter was expressed by cotransfection of an expression vector for Tax or in Cd2+‐treated JPX‐9 cells. Furthermore, immunohistochemical staining demonstrated that ATL cells infiltrating gastrointestinal tract were frequently positive for CCR9. Collectively, CCR9 is inducible in ATL cells expressing Tax and may play a role in the gastrointestinal involvement of ATL. © 2006 Wiley‐Liss, Inc.

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Human T-Cell Lymphotropic Virus Type I-Infected Cells Extravasate through the Endothelial Barrier by a Local Angiogenesis-Like Mechanism

Abstract: ABSTRACT

Cited by 49 publications

References 41 publications

Tight Junction Protein Claudin-1 Enhances the Invasive Activity of Oral Squamous Cell Carcinoma Cells by Promoting Cleavage of Laminin-5 γ2 Chain via Matrix Metalloproteinase (MMP)-2 and Membrane-Type MMP-1

Tight Junction Protein Claudin-1 Enhances the Invasive Activity of Oral Squamous Cell Carcinoma Cells by Promoting Cleavage of Laminin-5 γ2 Chain via Matrix Metalloproteinase (MMP)-2 and Membrane-Type MMP-1

Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes

Expression of CCR9 in HTLV‐1⁺ T cells and ATL cells expressing Tax

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Human T-Cell Lymphotropic Virus Type I-Infected Cells Extravasate through the Endothelial Barrier by a Local Angiogenesis-Like Mechanism

Abstract: ABSTRACT

Cited by 49 publications

References 41 publications

Tight Junction Protein Claudin-1 Enhances the Invasive Activity of Oral Squamous Cell Carcinoma Cells by Promoting Cleavage of Laminin-5 γ2 Chain via Matrix Metalloproteinase (MMP)-2 and Membrane-Type MMP-1

Tight Junction Protein Claudin-1 Enhances the Invasive Activity of Oral Squamous Cell Carcinoma Cells by Promoting Cleavage of Laminin-5 γ2 Chain via Matrix Metalloproteinase (MMP)-2 and Membrane-Type MMP-1

Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes

Expression of CCR9 in HTLV‐1+ T cells and ATL cells expressing Tax

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Expression of CCR9 in HTLV‐1⁺ T cells and ATL cells expressing Tax