Human T-Cell Leukemia Virus Type 3 (HTLV-3) and HTLV-4 Antisense-Transcript-Encoded Proteins Interact and Transactivate Jun Family-Dependent Transcription via Their Atypical bZIP Motif

Larocque, Émilie; André-Arpin, Charlotte; Borowiak, Malgorzata; Lemay, Guy; Switzer, William M.; Dodon, Madeleine Duc; Mesnard, Jean-Michel; Barbeau, Benoı̂t

doi:10.1128/jvi.01094-14

Cited by 10 publications

(8 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3(a), the linearization step still produces a band of the right molecular weight (lanes 1-3), with total elimination of non-specific amplification products (lanes 4-6). In contrast, amplification of unpurified cDNA obtained by RT of linearized RNA in the absence of the RT primer still produces a band (lane 7). No signals were detected in RT minus controls (Fig.…”

Section: Development Of a Modified Rt-pcr Protocol For Antisense Rna mentioning

confidence: 90%

See 1 more Smart Citation

Detection of antisense protein (ASP) RNA transcripts in individuals infected with human immunodeficiency virus type 1 (HIV-1)

Mancarella

Procopio

Achsel

et al. 2019

Journal of General Virology

View full text Add to dashboard Cite

The detection of antisense RNA is hampered by reverse transcription (RT) non-specific priming, due to the ability of RNA secondary structures to prime RT in the absence of specific primers. The detection of antisense RNA by conventional RT-PCR does not allow assessment of the polarity of the initial RNA template, causing the amplification of non-specific cDNAs. In this study we have developed a modified protocol for the detection of human immunodeficiency virus type 1 (HIV-1) antisense protein (ASP) RNA. Using this approach, we have identified ASP transcripts in CD4+ T cells isolated from five HIV-infected individuals, either untreated or under suppressive therapy. We show that ASP RNA can be detected in stimulated CD4+ T cells from both groups of patients, but not in unstimulated cells. We also show that in untreated patients, the patterns of expression of ASP and env are very similar, with the levels of ASP RNA being markedly lower than those of env. Treatment of cells from one viraemic patient with a-amanitin greatly reduces the rate of ASP RNA synthesis, suggesting that it is associated with RNA polymerase II, the central enzyme in the transcription of protein-coding genes. Our data represent the first nucleotide sequences obtained in patients for ASP, demonstrating that its transcription indeed occurs in those HIV-1 lineages in which the ASP open reading frame is present.

show abstract

Section: Development Of a Modified Rt-pcr Protocol For Antisense Rna mentioning

confidence: 90%

“…An antisense protein called Aph-2 has also been found in HTLV-2 [5], and is seemingly implicated in the regulation of viral transcription and translation. Antisense proteins Aph-3 and Aph-4 have been also described in the regulation of HTLV-3 and HTLV-4 transcription [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Detection of antisense protein (ASP) RNA transcripts in individuals infected with human immunodeficiency virus type 1 (HIV-1)

Mancarella

Procopio

Achsel

et al. 2019

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…As we saw above, antisense transcription is not exclusive to HIV-1. Indeed, not only some other lentiviruses (FIV, BIV), deltaretroviruses (HTLV-1, HTLV-2, HTLV-3, HTLV-4, STLV-1,and BLV), and gammaretroviruses (MLV) but notably some phylogenetic divergent viruses as Herpesviridae are capable of so-called antisense transcription ( Spivack and Fraser, 1987 ; Larocca et al, 1989 ; Flemington and Speck, 1990 ; Cheung, 1991 ; Holden et al, 1992 ; Prang et al, 1995 ; Perng et al, 1996 , 2002 ; Speck et al, 1997 ; Jiang et al, 1998 ; Borchers et al, 1999 ; Ciacci-Zanella et al, 1999 ; Briquet et al, 2001 ; Gaudray et al, 2002 ; Inman et al, 2004 ; Rajčáni et al, 2004 ; Bego et al, 2005 ; Calattini et al, 2005 , 2006 ; Jones et al, 2006 ; Ahn et al, 2007 , 2010 ; Ou et al, 2007 ; Duellman et al, 2009 ; Halin et al, 2009 ; Rasmussen et al, 2010 ; Larocque et al, 2011 , 2014 ; Barbeau et al, 2013 ; Miura et al, 2013 ; Barbeau and Mesnard, 2015 ; Barez et al, 2015 ; Daskalogianni et al, 2015 ; Liu et al, 2015 ; Durkin et al, 2016 ; Fochi et al, 2018 ; Moldován et al, 2018 ; Harrod, 2019 ; Jégado et al, 2019 ; Martinez et al, 2019 ; Tagaya et al, 2019 ; Hau et al, 2020 ; Matsuoka and Mesnard, 2020 ). In cell lines infected with laboratory-adapted FIV isolates and in various lymphoid tissues of cats infected by a FIV primary isolate, antisense transcripts arising from an antisense ORF that is complementary to the FIV env gene were detected ( Briquet et al, 2001 ).…”

Section: Functions Of Antisense Transcripts and Antisense Proteins Inmentioning

confidence: 99%

Antisense Transcripts and Antisense Protein: A New Perspective on Human Immunodeficiency Virus Type 1

et al. 2021

Self Cite

View full text Add to dashboard Cite

It was first predicted in 1988 that there may be an Open Reading Frame (ORF) on the negative strand of the Human Immunodeficiency Virus type 1 (HIV-1) genome that could encode a protein named AntiSense Protein (ASP). In spite of some controversy, reports began to emerge some years later describing the detection of HIV-1 antisense transcripts, the presence of ASP in transfected and infected cells, and the existence of an immune response targeting ASP. Recently, it was established that the asp gene is exclusively conserved within the pandemic group M of HIV-1. In this review, we summarize the latest findings on HIV-1 antisense transcripts and ASP, and we discuss their potential functions in HIV-1 infection together with the role played by antisense transcripts and ASPs in some other viruses. Finally, we suggest pathways raised by the study of antisense transcripts and ASPs that may warrant exploration in the future.

show abstract

“…and Ir19 might possibly be sequestered by the interaction with viral proteins, thereby impeding restriction of viral replication. shown to bind to various AP-1 components, including JunD, with variable impact on transcription depending on the factor and the HTLV subtype [74][75][76]. AP-1 dimers selectively recognize different DNA motifs, depending on the identity of the dimer, and interact cooperatively with other transcription factors.…”

Section: Signal Transduction / Transcriptional Response / Protein Degmentioning

confidence: 99%

“…While expression of AP-1 is regulated in a wide variety of viral infections, few binary interactions between viral proteins and AP-1 family members have been documented. Nevertheless, several proteins (bZIP, APH-2, -3, -4) expressed by the human T-cell leukemia virus (HTLV) have been shown to bind to various AP-1 components, including JunD, with variable impact on transcription depending on the factor and the HTLV subtype [74][75][76]. AP-1 dimers selectively recognize different DNA motifs, depending on the identity of the dimer, and interact cooperatively with other transcription factors.…”

Section: Signal Transduction / Transcriptional Response / Protein Degmentioning

confidence: 99%

Exploration of Binary Protein-Protein Interactions Between Tick-Borne Flaviviruses and Ixodes Ricinus

LEMASSON

CAIGNARD

UNTERFINGER

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundLouping ill virus (LIV) and tick-borne encephalitis virus (TBEV) are tick-borne flaviviruses that are both transmitted by the major European tick, Ixodes ricinus. Despite the importance of I. ricinus as an arthropod vector, its capacity to acquire and subsequently transmit viruses, known as vector competence, is poorly understood. At the molecular scale, vector competence is governed in part by binary interactions established between viral and cellular proteins within infected tick cells.MethodsTo investigate virus-vector protein-protein interactions (PPIs), the entire set of open reading frames for LIV and TBEV was screened against an I. ricinus cDNA library established from three embryonic tick cell lines using yeast two-hybrid methodology (Y2H). PPIs revealed for each viral bait were retested in yeast by applying a gap repair (GR) strategy and notably against the cognate protein of both viruses, to determine whether the PPIs were specific for a single virus or common to both. The interacting tick proteins were identified by automatic BLASTX and in silico analyses were performed to expose the biological processes targeted by LIV and TBEV.ResultsFor each virus, we identified 24 different PPIs involving six viral proteins and 22 unique tick proteins, with all PPIs being common to both viruses. According to our data, several viral proteins (pM, M, NS2A, NS4A, 2K and NS5) target multiple tick protein modules implicated in critical biological pathways. Of note, the NS5 and pM viral proteins establish PPI with several tumor necrosis factor (TNF) receptor-associated factor (TRAF) proteins, which are essential adaptor proteins at the nexus of multiple signal transduction pathways.ConclusionWe provide the first description of the TBEV/LIV-I. ricinus PPI network, and indeed of any PPI network involving a tick-borne virus and its tick vector. While further investigation will be needed to elucidate the role of each tick protein in the replication cycle of tick-borne flaviviruses, our study provides a foundation for understanding the vector competence of I. ricinus at the molecular level. Indeed, certain PPIs may represent molecular determinants of vector competence of I. ricinus for TBEV and LIV, and potentially for other tick-borne flaviviruses.

show abstract

Human T-Cell Leukemia Virus Type 3 (HTLV-3) and HTLV-4 Antisense-Transcript-Encoded Proteins Interact and Transactivate Jun Family-Dependent Transcription via Their Atypical bZIP Motif

Cited by 10 publications

References 67 publications

Detection of antisense protein (ASP) RNA transcripts in individuals infected with human immunodeficiency virus type 1 (HIV-1)

Detection of antisense protein (ASP) RNA transcripts in individuals infected with human immunodeficiency virus type 1 (HIV-1)

Antisense Transcripts and Antisense Protein: A New Perspective on Human Immunodeficiency Virus Type 1

Exploration of Binary Protein-Protein Interactions Between Tick-Borne Flaviviruses and Ixodes Ricinus

Contact Info

Product

Resources

About