Human T cell clones specific for tetanus toxoid: Characterization of antigen specificity and HLA restriction

Abstract

T cell blasts isolated from six-day cultures of peripheral blood mononuclear cells stimulated with tetanus toxoid (TT) were cloned in a two-layer agar system in the presence of autologous irradiated PBM (iPBM) and TT. Colonies were individually isolated and expanded in interleukin 2-containing medium. The antigen specificity of three T cell clones was attested by their capacity to proliferate under restimulation by TT and not by an unrelated antigen. The clones were specific for either the alpha or the beta chain of the toxin. T cells from these clones expressed Ia determinants and antigens of the helper/inducer T cell subset as defined by anti-T monoclonal antibodies. In the case of the alpha chain-specific clone MA 11 from the donor MA, allogeneic iPBM from HLA-compatible unrelated donors, including seven donors sharing one HLA DR specificity with MA, were found inefficient as antigen-presenting cells. A familial study, however, demonstrated that antigen presentation could be obtained using nonautologous cells. The presenting capability of cells from relatives of the donor MA segregated in association with the HLA-DRw6-bearing maternal haplotype present in MA. Results suggest that MA 11 cells recognized the antigen in the context of a surface determinant closely linked to HLA-DRw6.

Mycobacterium bovis BCG-induced human T-cell clones from BCG-vaccinated healthy subjects: antigen specificity and lymphokine production

“…The phenotype analysis of the clones showed that all but one of the clones were CD4+. Predominance of CD4+ clones has also been reported with' other antigens like tetanus toxoid (18), influenza virus (8), and Chlamydia trachomatis (16). The clones'were also expressing NG1 and NG2 subsets of HLA-DR, DP-associated antigens, and DQ locus antigens.…”

Stimulation of a human T-cell clone with anti-CD3 or tumor necrosis factor induces NF-kappa B translocation but not human immunodeficiency virus 1 enhancer-dependent transcription.

“…Clone D106 (a kind gift from M. Agrapart and J. J. Ballet, Hopital St. Louis, Paris) is a human CD4-positive, CD3-positive, CD8-negative T-cell clone that specifically responds to tetanus toxoid when cocultured with autologous macrophages (11). Clone SPB21 is a CD4-positive, CD3-positive, CD8-negative human T-cell clone that was selected through specific proliferation triggered by anti-T-cell receptor (TCR)-CD3 monoclonal antibody (mAb) 3D6 (12).…”

Antibodies against the light chain of tetanus toxin in human sera

“…No human monoclonal antibodies which are specific for light chain have been described yet, although most monoclonal antibodies have not been tested for this property (3,11). However, light chain-specific human T-cell clones have been described (22).…”