Human stratum corneum lipid organization as observed by atomic force microscopy on Langmuir–Blodgett films

Norlén, Lars; Gil, I. Plasencia; Simonsen, Adam Cohen; Descouts, P.

doi:10.1016/j.jsb.2006.12.006

Cited by 37 publications

(41 citation statements)

References 39 publications

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“…4C, D ). FA chain length of the stratum corneum is decreased in the ACBP ؊ / ؊ mice As described previously, lateral organization of membranes composed of stratum corneum lipids is highly dependent on both pH ( 54 ) and lipid composition ( 55 ). We therefore determined the overall lipid composition of the stratum corneum and epidermis.…”

Section: Local Proton Activity In Acbp ؊ / ؊ Mouse Stratum Corneum Ismentioning

confidence: 93%

“…First, investigations in reconstituted skin lipid mixtures have demonstrated that the FA chain length distribution dramatically infl uences lipid membrane lateral organization ( 55 ). Second, knockout of FA elongases expressed in the epidermis has been shown to cause impairment of the epidermal barrier ( 63-66 ).…”

Section: Acbp ؊ / ؊ Mice Display Impaired Epidermal Permeability Barrmentioning

confidence: 99%

“…Subtle changes in the permeability properties of the membranes participating in the barrier can be caused by lipid compositional changes. For example, it has been reported that the characteristic FFA composition of the stratum corneum membranes is a very important factor in promoting hydrocarbon chain mixing, defi ning lipid mixing properties and membrane stability ( 55,67 ). Similarly, the increased apparent pH detected in the stratum corneum of ACBP Ϫ / Ϫ mice may also modulate the stability of the membranes by enhancing ionization of the FAs.…”

Section: Acbp ؊ / ؊ Mice Display Impaired Epidermal Permeability Barrmentioning

confidence: 99%

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The acyl-CoA binding protein is required for normal epidermal barrier function in mice

Bloksgaard

Bek

Marcher

et al. 2012

Journal of Lipid Research

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This article is available online at http://www.jlr.org Supplementary key words stratum corneum • very long chain fatty acids • mono alkyl diacyl glycerol • transepidermal water loss • multiphoton excitation microscopy • lipid mass spectrometryThe acyl-CoA binding protein (ACBP)/diazepam binding inhibitor (DBI) (Entrez Gene ID: 13167) is a 10 kDa intracellular protein that specifi cally binds medium and long chain acyl-CoA esters (C 14 -C 22 ) with very high affi nity (K d ‫ف‬ 1-10 nM) ( 1, 2 ). The protein is expressed in all eukaryotic species and in all mammalian tissues investigated ( 3-5 ); however, expression levels differs markedly between different tissues and cell types examined, with particularly high levels in epithelial cell types and in cells with a high turn-over of fatty acids (FA) ( 6 ). Consistently with this, we have shown that the ACBP gene is activated by lipogenic transcription factors, such as the peroxisome proliferatoractivated receptor ␥ ( 7 ) and members of the sterol-regulatory element binding protein family ( 7-10 ). In vitro studies indicate that ACBP plays a role in transport of acyl-CoA esters and may deliver acyl-CoA esters to phospholipid

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Section: Local Proton Activity In Acbp ؊ / ؊ Mouse Stratum Corneum Ismentioning

confidence: 93%

Section: Acbp ؊ / ؊ Mice Display Impaired Epidermal Permeability Barrmentioning

confidence: 99%

Section: Acbp ؊ / ؊ Mice Display Impaired Epidermal Permeability Barrmentioning

confidence: 99%

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The acyl-CoA binding protein is required for normal epidermal barrier function in mice

Bloksgaard

Bek

Marcher

et al. 2012

Journal of Lipid Research

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“…Dioleoylphosphatidylcholine (DOPC), egg SM (SM), C 16:0 -SM, cholesterol, C 16:0 -ceramide, C 16:0 -SM-d 31 , and C 16:0 -ceramide-d 31 were purchased from Avanti Polar Lipids and were used as received. DOPC was purchased as a chloroform solution at a concentration of 10 mg/ml in ampule sealed under nitrogen and was stored at -20°C.…”

Section: Methodsmentioning

confidence: 99%

“…Langmuir-Blodgett monolayers comprised of ceramides, chol, and fatty acids that are used to model the skin strateum corneum composition exhibit ceramide-rich domains for a variety of lipid ratios. [29][30][31] Other studies have examined the influence of ceramide structure and surface pressure on the miscibility of mixed ceramide phosphatidylcholine monolayers. 32,33 The formation of a ceramide-enriched phase has been observed for chol/ceramide monolayers 34 and for SM monolayers at the air-water interface after treatment with SMase enzyme.…”

Section: 15mentioning

confidence: 99%

Chemical Mapping of Ceramide Distribution in Sphingomyelin-Rich Domains in Monolayers

Popov

Vobornik

Coban³

et al. 2008

Langmuir

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The incorporation of ceramide in phase-separated monolayers of ternary lipid mixtures has been studied by a combination of atomic force microscopy (AFM), fluorescence, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Replacement of a fraction of the sphingomyelin by ceramide in DOPC/SM/cholesterol monolayers leads to changes in the SM-cholesterol-rich liquid-ordered domains. AFM shows the formation of heterogeneous domains with small raised islands that are assigned to a ceramide-rich gel phase. ToF-SIMS provides conclusive evidence for the localization of SM and ceramide in ordered domains and shows that ceramide is heterogeneously distributed in small islands throughout the domains. The results indicate the utility of combining AFM and ToF-SIMS for understanding compositions of phase-separated membranes.

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Lipid Membrane Technology

Bagatolli

2009

Digital Encyclopedia of Applied Physics

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Human stratum corneum lipid organization as observed by atomic force microscopy on Langmuir–Blodgett films

Cited by 37 publications

References 39 publications

The acyl-CoA binding protein is required for normal epidermal barrier function in mice

The acyl-CoA binding protein is required for normal epidermal barrier function in mice

Chemical Mapping of Ceramide Distribution in Sphingomyelin-Rich Domains in Monolayers

Lipid Membrane Technology

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