Human Stem Cells from the Apical Papilla Response to Bacterial Lipopolysaccharide Exposure and Anti-inflammatory Effects of Nuclear Factor I C

Zhang, Jing; Zhang, Yaqing; Lv, Haipeng; Yu, Qing; Zhou, Zeyuan; Zhu, Qinglin; Wang, Zhihua; Cooper, Paul R.; Smith, Anthony J.; Niu, Ziru; Wang, He

doi:10.1016/j.joen.2013.07.018

Cited by 48 publications

(32 citation statements)

References 35 publications

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“…Expression of IL-8 at both gene and protein level was significantly high at 1 mg/mL LPS concentration and improved with increasing treatment time, as shown in other report (Zhang et al, 2013). Interestingly, IL8 was not induced at low concentrations (0.25 and 0.5 mg/mL) of LPS in the tested DPSCs population, indicating that inflammatory processes were probably triggered at concentration >1 mg/mL (Morsczeck et al, 2012).…”

Section: Discussionsupporting

confidence: 80%

Immune responses of human dental pulp stem cells in lipopolysaccharide‐induced microenvironment

Bindal

Ramasamy

Kasim

et al. 2018

Cell Biology International

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This study aimed to investigate the effect of inflammatory stimuli on dental pulp stem cells (DPSCs) by assessing their proliferation and expression of genes as well as proteins in lipopolysaccharide (LPS)-induced microenvironment (iDPSCs). DPSCs were first characterized for their mesenchymal properties prior to challenging them with a series of LPS concentrations from 12 to 72 h. Following to this, their proliferation and inflammatory based genes as well as protein expression were assessed. iDPSCs had demonstrated significant expression of mesenchymal markers. Upon exposure to LPS, the viability dropped distinctly with increasing concentration, as compared to control (P < 0.05). The expression of pro-inflammatory genes such as interleukin 6, interleukin 8 were augmented with exposure to LPS (P < 0.05). Similarly, cytokines like tumour necrosis factor (TNF) α and interleukin 1α had increased in dose dependant manner upon LPS exposure (P < 0.05). Our results suggest that LPS concentration between 1 and 2 μg/mL demonstrated inflammation induction in DPSCs that may simulate inflamed microenvironment of dental pulp in clinical scenario. Thus, optimizing iDPSCs secretome profile could be a promising approach to test various regenerative protocols in inflamed microenvironment.

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Section: Discussionsupporting

confidence: 80%

Immune responses of human dental pulp stem cells in lipopolysaccharide‐induced microenvironment

Bindal

Ramasamy

Kasim

et al. 2018

Cell Biology International

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“…The other is pTreg or iTreg, which develop from naive CD4 + T cells in the periphery outside of the thymus (Lee & Lee 2018). The anti-inflammatory and immunosuppressed properties of MSCs in the periapical area may also contribute to tissue regeneration and wound healing (Zhang et al 2013, Araujo-Pires et al 2014. Liu et al (2015) reported that systemic infusion of BMMSCs markedly enhances bone regeneration by upregulating Tregs.…”

Section: Discussionmentioning

confidence: 99%

Potential immunomodulatory effects of stem cells from the apical papilla on Treg conversion in tissue regeneration for regenerative endodontic treatment

Liu

et al. 2019

Int Endodontic J

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Aim To evaluate the expression of Foxp3‐positive lymphocytes around newly formed tissue after regenerative endodontic treatment (RET) in vivo and investigate the effects of stem cells from the apical papilla (SCAP) on the conversion of CD4+CD25− T cells to CD4+CD25+Foxp3+ regulatory T cells (Tregs) in vitro. Methodology Three 6‐month‐old beagles with nine doubled‐rooted premolars in each dog were randomly assigned to the RET group and the control group. RET was performed after apical periodontitis had been induced in the experimental immature teeth. Three months later, the expression of Foxp3 was detected in the histological sections by immunofluorescent staining. Human SCAP and CD4+CD25− T cells from mice spleens (1 : 1 and 1 : 5) were co‐cultured in cell–cell contact or in Transwells, respectively, for 24 and 72 h in vitro. The percentage of Tregs was evaluated by flow cytometry. The results were analysed using the Fisher's exact test and analysis of variance. P < 0.05 was regarded as statistically significant. Results Inflammatory cells were present with tissue regeneration in the RET group, and Foxp3‐positive T cells were enriched around the newly formed tissues. SCAP promoted Treg conversion after 72 h in vitro. Cell–cell contact played an important role after the 24 h co‐culture, whilst soluble factors were also involved after 72 h (P < 0.05). Conclusions SCAP promoted the conversion of pro‐inflammatory T cells to Tregs in vitro. Tregs were enriched around the regenerating tissues in the root canals after RET, which may create a suitable immune microenvironment for the differentiation of SCAP. This study provides an underlying mechanism for tissue regeneration during RET.

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“…The growth factors FGF2 and VEGF have also been shown to promote the proliferation, migration and adhesion of human periodontal ligament stem cells in vitro (Zhang et al . ). Ox‐LDL has also been shown to increase the migration and adhesive behaviour of BMSCs via upregulation of MCP‐1 expression (Zhang et al .…”

Section: Discussionmentioning

confidence: 97%

“…Furthermore, CXCR4 overexpression in BMSCs demonstrated enhanced migration in skin wounds in a SDF-1expression-dependent manner, subsequently reducing healing times (Yang et al 2013). The growth factors FGF2 and VEGF have also been shown to promote the proliferation, migration and adhesion of human periodontal ligament stem cells in vitro (Zhang et al 2013b). Ox-LDL has also been shown to increase the migration and adhesive behaviour of BMSCs via upregulation of MCP-1 expression (Zhang et al 2013a).…”

Section: Discussionmentioning

confidence: 99%

Effects of histone deacetylase inhibitors on regenerative cell responses in human dental pulp cells

Luo¹,

Wang²,

He³

et al. 2017

Int Endodontic J

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Human Stem Cells from the Apical Papilla Response to Bacterial Lipopolysaccharide Exposure and Anti-inflammatory Effects of Nuclear Factor I C

Cited by 48 publications

References 35 publications

Immune responses of human dental pulp stem cells in lipopolysaccharide‐induced microenvironment

Immune responses of human dental pulp stem cells in lipopolysaccharide‐induced microenvironment

Potential immunomodulatory effects of stem cells from the apical papilla on Treg conversion in tissue regeneration for regenerative endodontic treatment

Effects of histone deacetylase inhibitors on regenerative cell responses in human dental pulp cells

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