Human sperm DNA oxidation, motility and viability in the presence of l-carnitine during in vitro incubation and centrifugation

Banihani, Saleem Ali; Sharma, Rakesh K.; Bayachou, Mekki; Sabanegh, Edmund; Agarwal, Anju

doi:10.1111/j.1439-0272.2011.01216.x

Cited by 58 publications

(57 citation statements)

References 37 publications

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“…This result revealed that the dietary contains might effect on the testicular size of the birds. This is in agreement with previous findings that dietary energy level leads to an increase of testes weight, while high CP level gives a high testosterone level [1][2][3][4][5]. According to [7] sperm was produced by sertoli cells in tubules seminiferous and the number of sertoli cells is associated with testicular size and sperm production.…”

Section: Resultssupporting

confidence: 81%

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Effects of Energy-Protein Balance in the Diet on Semen Characteristic of West Java Local Ducks

Wahyuni¹,

Setiawan²,

Sujana³

2017

KLS

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Cihateup (West Java highland) and Rambon (West Java lowland) ducks are two local ducks which are commonly raised by farmers in West Java under extensive systems.The decline of land availability as a result of increasing human population has meant that the ducks should be raised under intensive system. An experimental research was conducted to evaluate the effect of energy-protein balance in the diet on semen characteristic of Cihateup and Rambon drakes under restricted water system (intensive system). The research was designed in Completely Randomized Design; there were six energy-protein balance in the diets as treatment, which were G 1 (3000 kcal/kg ME, 20% CP); G 2 (3000 kcal/kg ME, 16% CP); G 3 (3000 kcal/kg ME, 13.5% CP); G 4 (2700 kcal/kg ME, 20% CP); G 5 (2700 kcal/kg ME, 16% CP); and G 6 (2700 kcal/kg ME, 13.5% CP); each treatment was replicated three times. Semen was collected when the drakes reached the age of nine months and semen evaluation for volume semen, sperm motility and sperm abnormality was conducted. The results show that semen characteristics show that the highest motility was produced by G 5 (2700 kcal/kg ME, 16% CP) for Cihateup drakes and G 6 (2700 kcal/kg ME, 13.5% CP) for Rambon drakes. There was no significant different effect among treatments on abnormality of Cihateup drakes semen; anyhow G 5 (2700 kcal/kg ME, 16% CP) can be considered as the best diet for Rambon drakes based on its semen abnormality. It can be concluded that at feeding diet containing similar Metabolizable Energy level, Cihateup drakes required higher protein level than Rambon drakes to produce a good quality of semen.

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Section: Resultssupporting

confidence: 81%

“…The obtained number of sperm was used in the sperm concentration formula according to WHO [4]. Sperm abnormality was evaluated using eosin-negrosin staining on semen smears [5]. Sperm abnormality defined in this study was the secondary abnormal sperm that is a loosened head or tail sperm.…”

Section: Semen Collection and Determination Of Semen Quality Traitsmentioning

confidence: 99%

Effects of Energy-Protein Balance in the Diet on Semen Characteristic of West Java Local Ducks

Wahyuni¹,

Setiawan²,

Sujana³

2017

KLS

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“…As can be observed, in case of the OxyDNA kit, a shift to the right of the entire sperm population in the test sample with respect to the negative control is present, suggesting a binding of BP-F to all cells. A marked shift of BP-F labelling in spermatozoa has been reported also by other authors (Banihani et al 2012). The lower amount of 8-OHdG with the immunofluorescence procedure could be due to a lower access of the antibody into the sperm nuclei with respect to the BP-F of the kit.…”

Section: Comparing Two Methods For 8-ohdg Detectionmentioning

confidence: 67%

Development of a specific method to evaluate 8-hydroxy,2-deoxyguanosine in sperm nuclei: relationship with semen quality in a cohort of 94 subjects

Cambi¹,

Tamburrino²,

Marchiani³

et al. 2013

Reproduction

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Oxidative stress (OS) is involved in many disoders including male infertility. Human spermatozoa are very sensitive targets of reactive oxygen species (ROS) and most sperm functions are impaired in the case of OS. In addition unbalanced production of ROS is considered one of the most important causes of sperm DNA fragmentation, a semen trait of infertile men. The relationship between oxidative damage and semen quality is partially controversial, probably due to the different methods and/or targets used to reveal the OS. In this study, by fluorescence microscopy and flow cytometry, we compared two methods to reveal 8-hydroxy,2-deoxyguanosine (8-OHdG), the hallmark of oxidative DNA damage: an immunofluorescence method and the commercial OxyDNA kit. We found that although both methods localized the labelling in sperm nuclei they yielded different measures, and only with the immunofluorescence method was the labelling specific for sperm 8-OHdG. The immunofluorescence method, coupled to flow cytometry, was thus selected to analyse the 8-OHdG content in semen samples from 94 subfertile patients and to investigate the relationship with semen quality. We found that the percentages of spermatozoa with 8-OHdG (meanGS.D., 11.4G6.9%) were related to sperm count (Pearson's correlation coefficient (r)ZK0.27, PZ0.04 (ANOVA and student's t-test)), motility (progressive: rZK0.22, PZ0.04; non-progressive: rZ0.25, PZ0.01), and normal morphology (rZK0.27, PZ0.01).In conclusion, we demonstrate that immunofluorescence/flow cytometry is a reliable and specific method to detect 8-OHdG at single-cell level and show that oxidative damage only partially overlaps poor semen quality, suggesting that it could provide additional information on male fertility with respect to routine semen analysis.

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“…The same group has shown that intracellular levels of calcium ions are important markers of sperm quality because of their direct relationship with fertility potential and sperm morphology [25]. In our previous work [26], we showed that LC at 0.5 mg/mL significantly increased the motility of human sperm (5 × 10 6 cell/mL) after in vitro incubation and centrifugation. Conversely, we showed that high LC concentration (50 mg/mL) is toxic to sperm and significantly decreases sperm motility, but we did not provide a clear link for such LC toxicity [26].…”

Section: Discussionmentioning

confidence: 92%

l-Carnitine is a calcium chelator: a reason for its useful and toxic effects in biological systems

Banihani

Bayachou

Alzoubi

2014

Journal of Basic and Clinical Physiology and Pharmacology

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Human sperm DNA oxidation, motility and viability in the presence of l-carnitine during in vitro incubation and centrifugation

Cited by 58 publications

References 37 publications

Effects of Energy-Protein Balance in the Diet on Semen Characteristic of West Java Local Ducks

Effects of Energy-Protein Balance in the Diet on Semen Characteristic of West Java Local Ducks

Development of a specific method to evaluate 8-hydroxy,2-deoxyguanosine in sperm nuclei: relationship with semen quality in a cohort of 94 subjects

l-Carnitine is a calcium chelator: a reason for its useful and toxic effects in biological systems

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