Human serum Zn-α2-glycoprotein in amniotic fluid

Jirka, M; Blanický, P.; Šrajer, Jindřich; Zwinger, A; Jirásek, J

doi:10.1016/0009-8981(78)90228-0

Cited by 11 publications

(8 citation statements)

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“…The amniotic fluid levels of these acute-phase reactants tend to increase during late pregnancy in response to increased oxidative stress, with the increases being particularly pronounced in obstetric disorders [35][36][37]. This, in turn, may account for the higher exogenous copper and zinc levels in the AP samples.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Evaluation of Three Washing Procedures for Minimizing Exogenous Trace Element Contamination in Fetal Scalp Hair of Various Obstetric Outcomes

Razagui

2008

Biol Trace Elem Res

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A comparative evaluation of three washing methods for removing exogenous elemental contamination from fetal scalp hair is described. A total of 12 samples, including 6 from normal pregnancies and 6 from abruptio placentae (AP) cases, were used. Each was divided into four subsamples, with one left unwashed for comparison, while three were subjected to one of the three washing methods. The first consisted of agitating hair for 5 min in sequential and equal measures (30 ml) of acetone and water, followed by rinsing with acetone, and the process repeated twice. In the second and third, hair was agitated for 30 min in aqueous solutions of sodium lauryl sulfate (0.5%, w/v), and Triton X-100 (0.5%, v/v), respectively, followed by sequential rinsing with deionized water and acetone. After drying and microwave-assisted digestion in concentrated nitric acid, the subsamples' concentrations of zinc, copper, cadmium, lead, mercury and selenium were determined using inductively coupled mass spectrometry. All washed subsamples, irrespective of washing method used, had lower elemental concentrations than the corresponding, unwashed subsamples. These differences, taken as presumptive indicators of exogenous contamination, showed only small variations (<5%) between the three washing methods, irrespective of element, mode of delivery, and obstetric outcome. However, the contamination levels elicited by each of the three methods varied in magnitude according to individual elements, with those of copper being highest, followed by those of zinc, cadmium, mercury, selenium, and lead. They also varied according to obstetric outcome, being highest in the AP subsamples, while in the normal pregnancy group, copper and zinc contamination levels in the subsamples from neonates delivered by elective caesarean section were higher than in those from normal-delivery neonates. The data from this study suggest that the three washing methods used have comparable efficacies in removing exogenous elemental contamination from fetal hair, further inferring that it is probably a simple biological matrix to decontaminate. Possible contributory factors are discussed and contrasted with those affecting adult hair.

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Section: Discussionmentioning

confidence: 99%

A Comparative Evaluation of Three Washing Procedures for Minimizing Exogenous Trace Element Contamination in Fetal Scalp Hair of Various Obstetric Outcomes

Razagui

2008

Biol Trace Elem Res

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“…, 1999). ZAG has been detected in various physiological and pathological fluids (Jirka et al. , 1978; Ohkubo et al.…”

Section: Discussionmentioning

confidence: 99%

“…Zn-a2 glycoprotein is a 43 kDa soluble glycoprotein, originally isolated from human plasma in 1961, which was named for its tendency to precipitate with zinc salts and its electrophoretic mobility similar to the a2-globulins (Burgi & Schmid, 1961;Sanchez et al, 1999). ZAG has been detected in various physiological and pathological fluids (Jirka et al, 1978;Ohkubo et al, 1990;Bundred et al, 1991;Sanchez et al, 1997). Acting as a lipid-mobilizing factor, ZAG is expressed and secreted in mammalian adipose tissue and is markedly upregulated with cancer cachexia (Hale et al, 2001;Sanders & Tisdale, 2004;Tisdale, 2004).…”

Section: Discussionmentioning

confidence: 99%

Con A‐binding protein Zn‐α2‐glycoprotein on human sperm membrane is related to acrosome reaction and sperm fertility

et al. 2011

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Fertilization, the recognition and fusion between spermatozoa and oocyte, involves various molecules on the spermatozoa and oocyte membranes. Concanavalin A (ConA)-binding proteins may be one of the molecules involved in mammal spermatozoa fertilization; however, their structure and function remain largely unknown. Here, we initially identified a ConA-binding protein, Zn-α2-glycoprotein (ZAG), involved in regulating the acrosome reaction (AR) of human spermatozoa. ZAG is localized on the pre-equatorial region covering the acrosome, neck and tail (some parts of middle piece and principal piece respectively) regions of the acrosome intact human spermatozoa, and disappears in the acrosomal region of the acrosome-reacted spermatozoa. Polyclonal antibodies against human recombinant ZAG significantly reduced the AR and sperm capability binding to human zona pellucida or penetration into zona-free hamster oocytes. Furthermore, assessment of the signaling pathways regulated by ZAG revealed that ZAG affects sperm AR through both the cAMP/PKA and PKC pathways. These results indicate that ZAG, which is present on the human sperm membrane, plays a critical role in the AR and subsequently, may be involved in sperm fertility.

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“…It has subsequently been detected in various physiological and pathological fluids (Jirka et al 1978, Ohkubo et al 1990, Bundred et al 1991, Sanchez et al 1997. Acting as a lipid-mobilizing factor, ZAG is expressed and secreted in mammalian adipose tissue and is markedly upregulated with cancer cachexia (Hale et al 2001, Sanders & Tisdale 2004, Tisdale 2004.…”

Section: Introductionmentioning

confidence: 99%

The role of Zn-α2 glycoprotein in sperm motility is mediated by changes in cyclic AMP

Qü¹,

Ying²,

Guo³

et al. 2007

Reproduction

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Sperm motility is essential for male reproduction or natural fertilization. The cyclic AMP (cAMP)/cAMP-dependent protein kinase A (PKA) signaling pathway is generally recognized as one of the significant signaling pathways in the regulation of mammalian spermatozoan motility. Since Zn-a2-glycoprotein (ZAG) activity in mammalian adipose tissue is mediated via the b 3 -adrenoreceptor, with upregulation of the cAMP pathway, we hypothesize that ZAG may play the same role in sperm motility regulation, a new factor of regulation of sperm motility. Therefore, the gene encoding human ZAG was cloned and polyclonal antibodies were generated, and then laser scanning confocal microscopy and flow cytometry were employed to identify this protein in human spermatozoa. The results showed that ZAG protein was mostly localized on the pre-equatorial region covering the acrosome, neck, and middle piece of the flagellum of spermatozoa. Furthermore, using computer-assisted sperm analysis, we found that anti-human ZAG antibodies could significantly reduce the motility of human swim-up spermatozoa after 90-or 120-min incubation (P!0.05 and P!0.01 respectively), together with the decreasing of intracellular cAMP and PKA levels. In conclusion, these data suggest that ZAG is present in human spermatozoa and may be involved in the regulation of sperm motility via the cAMP/PKA signaling pathway.

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Human serum Zn-α2-glycoprotein in amniotic fluid

Cited by 11 publications

References 2 publications

A Comparative Evaluation of Three Washing Procedures for Minimizing Exogenous Trace Element Contamination in Fetal Scalp Hair of Various Obstetric Outcomes

A Comparative Evaluation of Three Washing Procedures for Minimizing Exogenous Trace Element Contamination in Fetal Scalp Hair of Various Obstetric Outcomes

Con A‐binding protein Zn‐α2‐glycoprotein on human sperm membrane is related to acrosome reaction and sperm fertility

The role of Zn-α2 glycoprotein in sperm motility is mediated by changes in cyclic AMP

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