Human serum paraoxonase (PON1) activity in acute organophosphorous insecticide poisoning

In vitro inhibition effect of some coumarin compounds on purified human serum paraoxonase 1 (PON1)

“…According to these results, the addition of a benzene ring to structure increased lipophilicity of compounds and making them more active than other coumarin derivatives. For the comparison of benzimidazolium salts, we can classify them in two parts; (i) 7-hydroxy coumarin (3)(4)(5)(6)(7)(8) and (ii) 7,8-dihydroxy coumarin-bearing compounds (11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Some compounds are bearing same groups in their structures apart from coumarin scaffold.…”

“…So lactam derivatives and a coumarin were not hydrolyzed by PON1. In our study, synthesized compounds are bearing both hydroxy and azolium substituent so according to the results of our and previous studies, compounds [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] are not suitable substrate for PON1 and they inhibited PON1 effectively.…”

“…''PON'' name derives from one of its most commonly used in vitro substrates, paraoxon. hPON1 also acts as an antioxidant enzyme that is an in vivo bioscavenger 5 . Coumarin is a member of a class of compounds known as benzopyrones.…”

Effect of some analgesics on Paraoxonase-1 purified from human serum

“…Bioscavengers are enzymes or antibodies neutralising highly toxic OPs before they reach biological targets [44]. Indeed, hPON1 also acts as an antioxidant enzyme, and is an in vivo bioscavencer [18]. In the recent years, PON1, PON2 and PON3 have been shown to be members of a multigene family in mammals.…”

“…Recently, a decrease in PON-I activity has been determined in some oxidative stress-associated diseases [17]. PON1 acts as an antioxidant enzyme [18] by protecting low-density lipoproteins (LDL) from oxidative damage, which is known to be associated with many vascular diseases, including atherosclerosis [19]. PON1 activity reduces in cardiovascular diseases, such as diabetes mellitus, chronic renal failure, rheumatoid arthritis, hyperthyroidism and age-related macular degeneration [20][21][22][23][24][25][26][27][28].…”