Human Serum Albumin−Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA‐200c

Vural, Oznur Akbal; Yaman, Yesim Tugce; Bolat, Gülçin; Abacı, Serdar

doi:10.1002/elan.202060342

Cited by 8 publications

(4 citation statements)

References 71 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, the differential pulse voltammetry (DPV) measurements were performed with a pulse period of 0.5 s, a pulse width of 0.05 s, and a pulse amplitude of 25 mV. [ 62,63 ]…”

Section: Methodsmentioning

confidence: 99%

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

Wang

et al. 2022

Small

View full text Add to dashboard Cite

Abnormal expression of microRNA‐21 (miR‐21) is considered to be closely associated with the pathogenesis of colorectal cancer. However, great challenges do exist for the development of ultra‐sensitive biosensors to detect the abnormal expression of miR‐21 due to the low concentration in serum (fm level) at the early stage of colorectal cancer. Therefore, electric field force is used to rotate and rearrange random multi‐walled carbon nanotubes (MWCNTs) at the microscale to improve the active sites of the electrode in this study. The free‐standing MWCNTs are densely and high‐orderly embedded into the bare electrode along the direction of the electric field. Compared to the bare electrode, the peak‐current response of the free‐standing MWCNT electrode improves by 150 times in cyclic voltammetric measurement. A nano‐genosensor based on the free‐standing MWCNT electrode is developed for measuring miR‐21. The nano‐genosensor for miR‐21 shows an ultra‐high sensitivity of 48.24 µA µm−1, a wide linear range from 0.01 × 10−15 to 100 × 10−12 m, and a low detection limit of 1.2 × 10−18 m. The present nano‐genosensor shows superior performance for miR‐21 in human serum samples and demonstrates a potential application for the diagnosis of early stage colorectal cancer.

show abstract

“…Meanwhile, the differential pulse voltammetry (DPV) measurements were performed with a pulse period of 0.5 s, a pulse width of 0.05 s, and a pulse amplitude of 25 mV. [ 62,63 ]…”

Section: Methodsmentioning

confidence: 99%

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

Wang

et al. 2022

Small

View full text Add to dashboard Cite

show abstract

“…On the other hand, AuNPs can be easily modified with miRNA molecules, particularly due to the gold surface affinity to the thiol group [ 12 ]. Used as electrochemical or optical sensors in fluorescence, surface-enhanced Raman scattering (SERS), surface plasmon resonance (SPR), or colorimetry-based detection approaches, AuNPs can either be dispersed in the aqueous media [ 13 ] or immobilized on solid support [ 14 ]. The enhancement of AuNPs is widely used in the applications of biosensors in miRNA detection, achieving higher selectivity and sensitivity starting from sub-nanomolar levels [ 11 ].…”

Section: Nanomaterials Used As Substratesmentioning

confidence: 99%

“…Thermogravimetric analysis (TGA) is centered on the weight loss of a sample as a function of increased temperature. Due to the organic content, the characteristic weight loss profile before and after the conjugation is investigated [ 12 , 14 ]. Generally, TGA is useful to confirm the presence of organic molecules conjugated to the inorganic nanomaterial, as is also the case with spectroscopic techniques.…”

Section: Nanomaterials Used As Substratesmentioning

confidence: 99%

“…A complementary DNA probe modified with SH can be covalently bonded to the AuNP surface [ 14 , 29 , 41 ], and similarly, the same type of modification can be used for a conjugation on AgNPs [ 18 , 69 ], core–shell Au–Ag NPs [ 26 ], Au–Ag nanocages [ 20 ], AuNP–peptide nanotube composites [ 12 ], Au-coated conglomerates of superparamagnetic NPs [ 43 ], citrate-capped AuNPs electrostatically adsorbed to Fe 3 O 4 NPs [ 70 ], Ag nanoclusters on Au electrode [ 71 ], and hollow Au–Ag nanospheres [ 72 ].…”

Section: Conjugation Strategies Leading To Functionalizationmentioning

confidence: 99%

See 1 more Smart Citation

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Vilímová¹,

Hervé-Aubert²,

Chourpa³

2022

Molecules

View full text Add to dashboard Cite

Recently, microRNAs (miRNA) captured the interest as novel diagnostic and prognostic biomarkers, with their potential for early indication of numerous pathologies. Since miRNA is a short, non-coding RNA sequence, the sensitivity and selectivity of their detection remain a cornerstone of scientific research. As such, methods based on nanomaterials have emerged in hopes of developing fast and facile approaches. At the core of the detection method based on nanotechnology lie nanoprobes and other functionalized nanomaterials. Since miRNA sensing and detection are generally rooted in the capture of target miRNA with the complementary sequence of oligonucleotides, the sequence needs to be attached to the nanomaterial with a specific conjugation strategy. As each nanomaterial has its unique properties, and each conjugation approach presents its drawbacks and advantages, this review offers a condensed overview of the conjugation approaches in nanomaterial-based miRNA sensing. Starting with a brief recapitulation of specific properties and characteristics of nanomaterials that can be used as a substrate, the focus is then centered on covalent and non-covalent bonding chemistry, leading to the functionalization of the nanomaterials, which are the most commonly used in miRNA sensing methods.

show abstract

Impedimetric monitoring of microRNA-200c-3p using hydroxyapatite nanoparticles modified pencil graphite electrodes

Congur,

Erdem

2024

J Mater Sci

View full text Add to dashboard Cite

It is important to monitor miRNA-200c-3p as it can potentially serve as an important biomarker for respiratory diseases including COVID-19 and cancer. Despite the importance of microRNA monitoring, there are few previous studies for miRNA-200c-3p monitoring, and the application of hydroxyapatite nanoparticles (HaNP) in miRNA biosensors is quite limited. This study aims to fill this gap by utilizing the advantageous properties of HaNPs to develop a powerful strategy to detect microRNA-200c-3p. First, HaNPs were modified on the surface of pencil graphite electrodes (PGEs). Subsequently, hybridization between a phosphate-labeled miRNA-200c-3p-specific DNA probe and its complementary RNA target was carried out in the solution phase. The DNA-RNA hybrid forms were immobilized on the surface of the HaNP-PGEs and the impedimetric measurements were performed. The changes at the charge transfer resistance value (Rct) were evaluated in terms of the hybridization and optimization of the experimental conditions. The detection limits (DLs) were calculated as 0.12 µg/mL (16.19 nM) in phosphate buffer solution (PBS, pH 7.40) and 0.31 µg/mL (41.82 nM) in synthetic plasma. The selectivity of the developed biosensor was tested against miRNA-200c-5p and miRNA-141-3p. The results promise a significant improvement in public health in terms of a leap forward in the early diagnosis of many serious diseases.

show abstract

Human Serum Albumin−Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA‐200c

Cited by 8 publications

References 71 publications

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Impedimetric monitoring of microRNA-200c-3p using hydroxyapatite nanoparticles modified pencil graphite electrodes

Contact Info

Product

Resources

About