Human serum albumin chromatography by Cibacron Blue F3GA-derived microporous polyamide hollow-fiber affinity membranes

Kassab, A.; Yavuz, Handan; Odabaşı, Mehmet; Deni̇zli̇, Adil

doi:10.1016/s0378-4347(00)00311-x

Cited by 63 publications

(33 citation statements)

References 36 publications

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“…Porous membranes with well-defined pore structures and specifically functionalized surfaces play an important role in bioseparation, e.g., high-performance ultrafiltration fractionation [28] or affinity filtration of proteins. [29] Membrane adsorption is a developing bioseparation technique that has been studied for various proteins such as urokinase, [30] avidin, [31] human serum albumin [32] and immunoglobulin G. [33,34] In this study porous p(HEMA-co-MAAL) membranes were produced by the photopolymerization of HEMA and the newly synthesized comonomer MAAL. The non-specific adsorption of lysozyme on the pHEMA membranes was found to be 0.9 lg N cm -2 .…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Poly[(hydroxyethyl methacrylate)-co-(methacrylamidoalanine)] Membranes and Their Utilization as an Affinity Sorbent for Lysozyme Adsorption

et al. 2001

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Section: Resultsmentioning

confidence: 99%

Synthesis of Poly[(hydroxyethyl methacrylate)-co-(methacrylamidoalanine)] Membranes and Their Utilization as an Affinity Sorbent for Lysozyme Adsorption

et al. 2001

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“…The distortion of existing salt bridges in the presence of salt also contributed to this low protein adsorption at high ionic strength. [20] …”

Section: Effect Of Mah Contentmentioning

confidence: 99%

A Novel Affinity Support Material for the Separation of Immunoglobulin G from Human Plasma

Gari̇pcan

Deni̇zli̇²

2002

Macromol. Biosci.

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2‐Methacrylamidohistidine (MAH) as a pseudospecific ligand was synthesized from methacryl chloride and histidine. Spherical beads with an average size of 50–63 μm were obtained by the radical suspension polymerization of MAH and 2‐hydroxyethyl methacrylate (HEMA) conducted in an aqueous dispersion medium. Owing to the reasonably rough character of the bead surface, P(HEMA‐co‐MAH) beads had a specific surface area of 17.6 m2·g–1. Synthesized MAH was characterized by NMR. P(HEMA‐co‐MAH) beads were characterized by swelling studies, FT‐IR spectroscopy, scanning electron microscopy (SEM) and elemental analysis. P(HEMA‐co‐MAH) affinity beads with a swelling ratio of 65% were used in the separation of human immunoglobulin G (HIgG) from aqueous solutions and human plasma. The maximum HIgG adsorption on the P(HEMA‐co‐MAH) adsorbents was observed at pH 7.4 for phosphate and at pH 6.0 for morpholinoethanesulfonic acid buffers. The HIgG adsorption onto the PHEMA adsorbents was negligible. Higher adsorption values (up to 46.5 mg·g–1) were obtained when the P(HEMA‐co‐MAH) adsorbents were used in aqueous solutions. Much higher amounts of HIgG were adsorbed from human plasma (up to 73.8 mg·g–1). Adsorption capacities of other blood proteins were obtained as 3.2 mg·g–1 for fibrinogen and 4.6 mg·g–1 for albumin. The total protein adsorption was determined to be 82.2 mg·g–1. The pseudospecific affinity beads allowed one‐step separation of HIgG from human plasma. HIgG molecules could be repeatedly adsorbed and desorbed with these adsorbents without noticeable loss in their HIgG adsorption capacity.

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“…To separate the albumin, dye-affinity chromatography is commonly used, and Cibacron blue F3G-A (CB) is a ligand of high popularity [2]. In this context, CB is coupled to matrices of various forms, such as microspheres [3][4] and membranes [5][6], or used unbound for albumin extraction with a reversed micellar system [7]. The non-covalent CB-human serum albumin complex has been thoroughly investigated.…”

Section: Introductionmentioning

confidence: 99%

Interaction of serum albumin with vinyl sulfonate azo dye

Sereikaitė

Bumelis

2008

Open Chemistry

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Human serum albumin chromatography by Cibacron Blue F3GA-derived microporous polyamide hollow-fiber affinity membranes

Cited by 63 publications

References 36 publications

Synthesis of Poly[(hydroxyethyl methacrylate)-co-(methacrylamidoalanine)] Membranes and Their Utilization as an Affinity Sorbent for Lysozyme Adsorption

Synthesis of Poly[(hydroxyethyl methacrylate)-co-(methacrylamidoalanine)] Membranes and Their Utilization as an Affinity Sorbent for Lysozyme Adsorption

A Novel Affinity Support Material for the Separation of Immunoglobulin G from Human Plasma

Interaction of serum albumin with vinyl sulfonate azo dye

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