Human serum albumin as a new interacting partner of prolactin inducible protein in human seminal plasma

Kumar, Sanjay; Tomar, Amit; Singh, Sudhuman; Saraswat, Mayank; Singh, Sarman; Singh, T.P.; Yadav, Savita

doi:10.1016/j.ijbiomac.2011.12.015

Cited by 22 publications

(24 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…29 Prolactin inducible protein binds to many proteins, such as fibrinogen, actin, keratin, myosin, tropomyosin, 30 immunoglobulin G, 31 and human zinc-α-2 glycoprotein. 32 The ability of PIP to bind IgG-Fc suggests that it may have an immunomodulatory role. A low expression level of PIP in human seminal plasma is also suggested to be associated with infertility.…”

Section: ■ Discussionmentioning

confidence: 99%

Investigation of Male Infertility Using Quantitative Comparative Proteomics

Légaré¹,

Droit²,

Fournier³

et al. 2014

J. Proteome Res.

View full text Add to dashboard Cite

Male factors account for 40% of infertility cases. The identification of differentially expressed proteins on spermatozoa from fertile and infertile men can help in the elucidation of the molecular basis of male infertility. The aim of this study was to compare sperm proteomes from 3 different groups: fertile men, normozoospermic men consulting for infertility, and normozoospermic men with an impaired capacity for fertilization (IVF-failure). We used differential proteomics with isobaric tags for relative and absolute quantitation (iTRAQ) labeling, and LC-MS analysis to identify proteins that are differentially expressed. A total of 348 unique proteins were identified and quantified. The analysis identified 33 proteins that were differentially expressed in the IVF-failure group vs the fertile group. Comparison of the infertile and fertile groups revealed that 18 proteins appeared to be differentially expressed. Four proteins were similarly altered in the IVF-failure and infertile groups: semenogelin 1 (SEMG1), prolactin-induced protein (PIP), glyceraldehyde-3-phosphate dehydrogenase (GAPDHS), and phosphoglycerate kinase 2 (PGK2). These protein markers were selected for validation using multiple reactions monitoring mass spectrometry (MRM-MS) and further confirmed by Western blot analysis. Overall, these results suggest that a panel of proteins may be used as biomarkers for future studies of infertility.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Investigation of Male Infertility Using Quantitative Comparative Proteomics

Légaré¹,

Droit²,

Fournier³

et al. 2014

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

“…The function of PIP is not known, but it is suspected to play a role in host defense against pathogens [1][2][3][4][5][6] because it is expressed in tissues strategically located at several Correspondence: Dr. Jude E. Uzonna e-mail: jude.uzonna@med.umanitoba.ca ports of pathogen entry, including the skin, eyes, and mouth [7]. Although a definitive evidence showing a role for PIP in host immunity is lacking, its ability to potentially bind with several important host molecules including CD4 + T-cell receptor, IgG, actin, zinc α2-glycoprotein, fibronectin, and enamel pellicle suggests it may have important biological functions [8][9][10][11][12][13]. Indeed, it has been shown that PIP is a ligand of the CD4 molecule and a potent inhibitor of * These authors contributed equally to this manuscript.…”

mentioning

confidence: 99%

Deficiency of prolactin‐inducible protein leads to impaired Th1 immune response and susceptibility to Leishmania major in mice

Liu

Mou

et al. 2015

Eur J Immunol

View full text Add to dashboard Cite

Although the strategic production of prolactin-inducible protein (PIP) at several ports of pathogen entry into the body suggests it might play a role in host defense, no study has directly implicated it in immunity against any infectious agent. Here, we show for the first time that PIP deficiency is associated with reduced numbers of CD4 + T cells in peripheral lymphoid tissues and impaired CD4 + Th1-cell differentiation in vitro. In vivo, CD4+ T cells from OVA-immunized, PIP-deficient mice showed significantly impaired proliferation and IFN-γ production following in vitro restimulation. Furthermore, PIPdeficient mice were highly susceptible to Leishmani major infection and failed to control lesion progression and parasite proliferation. This susceptibility was associated with impaired NO production and leishmanicidal activity of PIP KO macrophages following IFN-γ and LPS stimulation. Collectively, our findings implicate PIP as an important regulator of CD4 + Th1-cell-mediated immunity. Keywords: IFN-γ r Leishmania r Macrophages r Protozoan r Th1 cellsAdditional supporting information may be found in the online version of this article at the publisher's web-site IntroductionThe mouse prolactin-inducible protein (PIP), also known as the mouse submaxillary gland protein, is a 14 kDa protein present in the saliva of mice. The function of PIP is not known, but it is suspected to play a role in host defense against pathogens [1][2][3][4][5][6] because it is expressed in tissues strategically located at several Correspondence: Dr. Jude E. Uzonna e-mail: jude.uzonna@med.umanitoba.ca ports of pathogen entry, including the skin, eyes, and mouth [7]. Although a definitive evidence showing a role for PIP in host immunity is lacking, its ability to potentially bind with several important host molecules including CD4 + T-cell receptor, IgG, actin, zinc α2-glycoprotein, fibronectin, and enamel pellicle suggests it may have important biological functions [8][9][10][11][12][13]. Indeed, it has been shown that PIP is a ligand of the CD4 molecule and a potent inhibitor of * These authors contributed equally to this manuscript.C 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu Eur. J. Immunol. 2015. 45: 1082-1091 Immunity to infection 1083 T-cell apoptosis induced by sequential CD4/T-cell receptor triggering, which in part modulates the immune response during viral infection by interfering with the functions mediated by the CD4 molecule [5,6,14]. Furthermore, PIP has been reported to exhibit a mitogenic effect for both normal and malignant breast epithelial cells [15], strongly suggesting that it might play a critical role in differentiation, proliferation, and function of immune cells.Our previous studies show that PIP KO mice have enlarged submandibular lymph nodes and thymic medulla [16]. However, to date, no studies have addressed the contributions of PIP in T-cell responses to either model Ags or infectious agents. Our major aim in the present study is to investigate whether PIP has immunoregulatory function...

show abstract

“…Interaction of PIP with ZAG has been correlated with modulation of sperm motility as ZAG is known to induce the sperm motility [18]. Recently, Kumar et al [5] have purified native complex of HSA-PIP from human SP and proposed that HSA might work to sequester PIP thus playing a role in relieving the inhibition of sperm motility as albumin has been known to preserve the motility of sperm.…”

Section: Resultsmentioning

confidence: 99%

“…We retrieved PPI network for human PIP and it lists 10 interacting partners, as per the last update of database in 2011. We reconstructed its PPI network by adding two more nodes for recently identified partners, HSA [5] and SEG1 fragments (identified in present study) (Fig. 4).…”

Section: Resultsmentioning

confidence: 99%