Human Sertoli-Spermatogenic Cell Cocultures Prepared from Biopsies of Cryptorchid Testes Performed During Orchidopexy

Tres, Laura L.; Mesrobian, Hrair-George J.; Abdullah, Munir; Kierszenbaum, Abraham L.

doi:10.1016/s0022-5347(17)41086-x

Cited by 8 publications

(12 citation statements)

References 24 publications

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“…Most of these studies, however, have been carried out in cells obtained from animals (rat, mouse, hamster, pig) but there is little information on the function of human testicular cells in culture (3,4), particularly of cells obtained from sexually immature subjects. We have attempted to develop a method of primary culture of human testicular cells obtained from testes of prepuber¬ tal boys, collected at necropsy.…”

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confidence: 99%

Primary culture of prepubertal human testicular cells isolated from testes collected at necropsy

Berensztein

Belgorosky²,

Rivarola³

1992

Acta Endocrinologica

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of prepubertal human testicular cells isolated from testes collected at necropsy. Acta Endocrinol 1992;127:66-71. ISSN 0001-5598The aim of the present work was to develop a method for maintaining prepubertal human testicular cells in culture. Seven pairs of testes of boys who died of causes unrelated to endocrine or metabolic diseases were obtained at necropsy. Histology of the testes was normal. Testes were digested with collagenase and dispersed cells were seeded in multi-well dishes in the presence of 5% bovine fetal serum. After the first day, cells were cultured for five days in serum-free medium in the presence or absence of 918 pmol/l insulin. At the end of culture, microscopic examination showed healthy looking cells with characteristics compatible with pre-Sertoli cells; peritubular cells were identified by immunocytochemistry. In the presence of insulin, cells were able to secrete either testosterone or estradiol into the medium, as well as to reveal aromatase activity. In order to study the effect of the time elapsed between death and beginning of cultures, steroidogenic activity was related to this post mortem time. It was found that, in the presence of insulin, cells obtained from testes with less than 24 h of post mortem time secreted testosterone (64\m=+-\7.2 pmol/106 cells\m=.\24h, mean\m=+-\sd) while cells obtained from testes with more than 24 h of post mortem time did not secrete testosterone. With long post mortem times, aromatase activity under insulin increased from non-detectable to 35 pmol/106 cells\m=.\24h. Time course studies showed that cells with capacity to secrete testosterone increase this secretion gradually up to day 10 of culture, while those with detectable aromatase activity showed increments in this activity during the first week of culture. It is concluded that it is possible to maintain cultures of prepubertal human testicular cells obtained at necropsy. In order to maintain testosterone secretion capacity, the time between death and initiation of culture should not exceed 24 h.During the last decade, many reports on studies of the function of the testicular cells maintained in primary culture have been published (1, 2). These studies have contributed to our understanding of the endocrine and paracrine interactions of these cells and to the identifica¬ tion of new secretory products and specific receptors.Most of these studies, however, have been carried out in cells obtained from animals (rat, mouse, hamster, pig) but there is little information on the function of human testicular cells in culture (3, 4), particularly of cells obtained from sexually immature subjects. We have attempted to develop a method of primary culture of human testicular cells obtained from testes of prepuber¬ tal boys, collected at necropsy. We are not aware of any reports using necropsy material for testicular cell cul¬ ture, but Ruokonen et al. (5) found no difference in steroid composition between testes of cadavers and those removed surgically. We have studied the capacity of testicular cells to ...

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confidence: 99%

Primary culture of prepubertal human testicular cells isolated from testes collected at necropsy

Berensztein

Belgorosky²,

Rivarola³

1992

Acta Endocrinologica

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“…Spermatogenic cell types present in human testicular samples were evaluated by light microscopy using the aceto-orcein staining method (Tres et al, 1989). Freshly ejaculated human sperm samples from healthy individuals were collected and frozen at -80°C.…”

Section: Materials and Methods Sample Collection And Preparation Formentioning

confidence: 99%

“…Cell density was verified by phase-contrast microscopy; cell types were identified by aceto-orcein staining of squashed preparations (Tres et al, 1989). For indirect immunofluorescence, resuspended cells (testicular cells or sperm) were placed on glass microscope slides, allowed to attach for 10 min by unit gravity sedimentation, and fixed for 15 min in 3% paraformaldehyde in 0.1 M cacodylic acid buffer, pH 7.2, containing 1 mM CaC1, and 1 mM MgC12.…”

Section: Indirect Immunofluorescencementioning

confidence: 99%

Galactosyl receptor in human testis and sperm is antigenically related to the minor C‐type (Ca²⁺‐dependent) lectin variant of human and rat liver

Goluboff¹,

Mertz²,

Tres³

et al. 1995

Molecular Reproduction Devel

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Galactosyl receptor, a cell surface Ca(2+)-dependent lectin with binding affinity for galactose, was evaluated by immunoblotting, immunoprecipitation, Northern blotting, and immunocytochemistry in human liver, testis, and sperm. Polyclonal antisera raised against the minor asialoglycoprotein receptor variant of rat hepatocytes (designated rat hepatic lectin-2/3, RHL-2/3), and its human liver-equivalent (designated H2), recognize native galactosyl receptor in the testis and sperm in immunoblotting, immunoprecipitation, and immunocytochemical experiments. An equivalent to the major hepatocyte asialoglycoprotein receptor variant (rat RHL-1 and human H1) was not detected. Human testis and sperm galactosyl receptor was resolved, after immunoprecipitation and immunoblotting, as a single protein component of molecular mass 50 kD. The single protein component in human testis and sperm contrasted with the doublet nature of rat testis and sperm galactosyl receptor, consisting of two components of molecular masses of 54 and 49 kD. Northern blotting experiments using radiolabeled H1 and H2 cDNA probes confirmed the presence of H2 mRNA and the lack of H1 mRNA in the human testis. Immunocytochemical studies detected specific antigenic sites on the entire surfaces of spermatogenic cells. However, immunoreactivity in epididymal and ejaculated sperm was confined to head surfaces overlying the acrosome. Results from these studies, and from previous studies in the rat, suggest that the testis/sperm galactosyl receptor is a C-type Ca(2+)-dependent lectin with possible roles in cell-cell interaction during spermatogenesis and sperm-zona pellucida binding at fertilization.

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“…The two aforementioned averaged values are related to the measure of the collective behaviour domain. The average group direction is often used as the group polarity (i.e., the degree of alignment) [4,15,29,78], whereas, the centre of mass is a useful measure for group clustering [6,14,[84][85][86]. These interactions result in keeping a certain distance (direction) with the global state.…”

Section: φ Values For Global Parameter Settings Across Timescalesmentioning

confidence: 99%

“…Global dynamic patterns in swarming [1][2][3][4][5][6], schooling (of fish) [7][8][9][10][11], flocking (of birds) [12][13][14][15][16][17], genes [18], proteins [19], or neural networks [20][21][22][23], emerge from local interactions between self-organising individuals or components. Despite their complexity, these collective systems are capable of processing information efficiently in critical conditions, for example, individuals making a swift response [14][15][16] or a group making a good decision [24][25][26] in a changing environment. Individual conflicts do not necessarily disrupt group integrity and instead contribute to their collective response [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

New Classification of Collective Animal Behaviour as an Autonomous System

Niizato¹,

Sakamoto²,

Mototake³

et al. 2020

Preprint

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Integrated information theory (IIT) was initially proposed to describe human consciousness in terms of intrinsic-causal brain network structures. This theory could potentially be used for conceptualising complex living systems. In a previous study, we analysed collective behaviour in {\it Plecoglossus altivelis}. We found that IIT 3.0 exhibits qualitative discontinuity between three and four schools of fish in terms of $\Phi$ values (i.e., group integrity). Other measures, such as mutual information, did not show such characteristics. In this study, we follow up on our previous findings and introduce two new factors. First, we define the global parameter settings to determine a different kind of group integrity. Second, we set several timescales (from $\Delta t =5/120$ s to $\Delta t =120/120$ s). The results showed that we succeeded in classifying fish school according to their group size in terms of the degree of group integrity, despite the small group size. The concrete classification includes the followership for a two-fish school, fission--fusion for a three-fish school, emergence of leadership for a four-fish school, and emergence of Boid-like behaviour for a five-fish school. These minute classifications have never been revealed before. Finally, we discuss one of the longstanding paradoxes in collective behaviour, known as the heap paradox, for which two tentative answers could be provided through our IIT analysis.

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Human Sertoli-Spermatogenic Cell Cocultures Prepared from Biopsies of Cryptorchid Testes Performed During Orchidopexy

Cited by 8 publications

References 24 publications

Primary culture of prepubertal human testicular cells isolated from testes collected at necropsy

Primary culture of prepubertal human testicular cells isolated from testes collected at necropsy

Galactosyl receptor in human testis and sperm is antigenically related to the minor C‐type (Ca²⁺‐dependent) lectin variant of human and rat liver

New Classification of Collective Animal Behaviour as an Autonomous System

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Human Sertoli-Spermatogenic Cell Cocultures Prepared from Biopsies of Cryptorchid Testes Performed During Orchidopexy

Cited by 8 publications

References 24 publications

Primary culture of prepubertal human testicular cells isolated from testes collected at necropsy

Primary culture of prepubertal human testicular cells isolated from testes collected at necropsy

Galactosyl receptor in human testis and sperm is antigenically related to the minor C‐type (Ca2+‐dependent) lectin variant of human and rat liver

New Classification of Collective Animal Behaviour as an Autonomous System

Contact Info

Product

Resources

About

Galactosyl receptor in human testis and sperm is antigenically related to the minor C‐type (Ca²⁺‐dependent) lectin variant of human and rat liver