Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture

Conceição, Rogério Arcuri; Ludovico, Marilúcia Santos; Andrade, Carlos Rogério; Yano, Tomomasa

doi:10.1590/s0100-879x2012007500057

Cited by 9 publications

(10 citation statements)

References 33 publications

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“…Higher expression of the gene encoding Ag43 was found in biofilm-forming bacteria, and a deletion of agn43 caused a decrease in biofilm formation (24,63). We also found high prevalences of agn43 in EAEC (75%), SAEC (62.5%), and UPEC (58.3%) isolates, which is in agreement with studies from other groups (65,66). A strong formation of aggregates, which is promoted by Ag43 (67), was observed while screening EAEC isolates for biofilm formation in different media with our VideoScan technology.…”

Section: Discussionsupporting

confidence: 91%

Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes

Schiebel

Böhm

Nitschke

et al. 2017

Appl Environ Microbiol

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Bacterial biofilm formation is a widespread phenomenon and a complex process requiring a set of genes facilitating the initial adhesion, maturation, and production of the extracellular polymeric matrix and subsequent dispersal of bacteria. Most studies on biofilm formation have investigated nonpathogenic K-12 strains. Due to the extensive focus on laboratory strains in most studies, there is poor information regarding biofilm formation by pathogenic isolates. In this study, we genotypically and phenotypically characterized 187 human clinical isolates representing various pathotypes (e.g., uropathogenic, enteropathogenic, and enteroaggregative ). We investigated the presence of biofilm-associated genes ("genotype") and phenotypically analyzed the isolates for motility and curli and cellulose production ("phenotype"). We developed a new screening method to examine the biofilm formation ability. In summary, we found a high prevalence of biofilm-associated genes. However, we could not detect a biofilm-associated gene or specific phenotype correlating with the biofilm formation ability. In contrast, we did identify an association of increased biofilm formation with a specific pathotype. Enteroaggregative (EAEC) was found to exhibit the highest capacity for biofilm formation. Using our image-based technology for the screening of biofilm formation, we demonstrated the characteristic biofilm formation pattern of EAEC, consisting of thick bacterial aggregates. In summary, our results highlight the fact that biofilm-promoting factors shown to be critical for biofilm formation in nonpathogenic strains do not reflect their impact in clinical isolates and that the ability of biofilm formation is a defined characteristic of EAEC. Bacterial biofilms are ubiquitous and consist of sessile bacterial cells surrounded by a self-produced extracellular polymeric matrix. They cause chronic and device-related infections due to their high resistance to antibiotics and the host immune system. In nonpathogenic , cell surface components playing a pivotal role in biofilm formation are well known. In contrast, there is poor information for their role in biofilm formation of pathogenic isolates. Our study provides insights into the correlation of biofilm-associated genes or specific phenotypes with the biofilm formation ability of commensal and pathogenic Additionally, we describe a newly developed method enabling qualitative biofilm analysis by automated image analysis, which is beneficial for high-throughput screenings. Our results help to establish a better understanding of biofilm formation.

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Section: Discussionsupporting

confidence: 91%

Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes

Schiebel

Böhm

Nitschke

et al. 2017

Appl Environ Microbiol

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“…It is well known that during biofilm formation, E. coli bacterial cells colonize on the surface through specific adhesin and curli [1,14,17]. Several authors have reported that curli promoting adhesion of the bacteria cells to the surface [18,19], and adhesion is the first step for biofilm development [20]. However, our results revealed that 4-5 mM EDTA alone inhibited curli formation and bacterial adhesion.…”

Section: Effect Of Edta and Drugs Biofilmcontrasting

confidence: 62%

Role of CSE1034 in Escherichia coli Biofilm Destruction

Kumar¹

2013

J Microb Biochem Technol

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Present study was conducted to assess the effect of ethylenediamine tetraacetic acid (EDTA); a non antibiotic adjuvant, and CSE1034, a novel antibiotic adjuvant entity in biofilm destruction of Escherichia coli, and comparing the efficacy with other drugs. We first determined the susceptibility of six antimicrobial agents against planktonic cultures, as well as sessile cells of E. coli clinical isolates, using the Clinical and Laboratory Standards Institute (CLSI) method. Subsequently, effects of EDTA alone, and drugs on bacterial curli production, adhesion, and in-vitro biofilm destruction were studied. The percentage of biofilm persistence was determined with spectrophotometry. The structural damage of biofilms was studied by scanning electron microscope. It was found that, among the drugs used, CSE1034 was the most effective against all of the E. coli clinical isolates, with MIC and MBEC values ranging from 32-64 µg/ml and 256-512 µg/ml, respectively. Exposure of clinical isolates with EDTA alone caused inhibitions of curli formation and bacterial adhesion at 4 to 5 mM. Further, EDTA treatment of preformed biofilm caused complete biofilm destruction at 8-10 mM. Interestingly, CSE1034 due to presence of 10 mM EDTA led to enhanced antibacterial, as well as biofilm destruction activities. Results of spectrophotometric analysis and scanning electron microscopy revealed that approximately 92% biofilms were eradicated by the CSE1034, and not by comparator drugs. The results indicate that the CSE1034 appears to be most effective drug of choice for the treatment of infections caused by E. coli.

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“…In the neonatal period, the infection is more common in boys (approximately 60%), whereas from the age of 2–3 months this tendency is reversed. As there are no characteristic clinical symptoms in this period of life, sometimes the first observed symptoms of UTI are psychosomatic disorders or hypertension [18, 19]. Clinical symptoms associated with UTI may have a different clinical picture, ranging from asymptomatic bacteriuria, various ascending infections (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Clinical symptoms associated with UTI may have a different clinical picture, ranging from asymptomatic bacteriuria, various ascending infections (e.g. acute pyelonephritis), up to severe urosepsis [18]. The most common etiologic pathogens associated with UTI include Gram-negative Enterobacteriaceae , with predominating strains of uropathogenic E. coli [19].…”

Section: Introductionmentioning

confidence: 99%

“…Urinary tract infections can often cause bacteremia, especially in hospitalized patients, when, due to catheter contamination, biofilm is produced by invasive ExPEC strains possessing many virulence factors [65–67]. In their analysis of genes determining production of adhesins by SEPEC strains, Conceição et al [18] have indicated that 98.0% of these SEPEC strains tested positive for the fimH , 69.4% for flu , 53.1% for csgA , 38.8% for vat , and 32.7% for iha . The authors have suggested that SEPEC adhesion to cell surfaces occurs with the involvement of non- fimH mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports

et al. 2019

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Extraintestinal pathogenic E . coli (ExPEC) are facultative pathogens that are part of the normal human intestinal flora. The ExPEC group includes uropathogenic E. coli (UPEC), neonatal meningitis E. coli (NMEC), sepsis-associated E. coli (SEPEC), and avian pathogenic E. coli (APEC). Virulence factors (VF) related to the pathogenicity of ExPEC are numerous and have a wide range of activities, from those related to bacteria colonization to those related to virulence, including adhesins, toxins, iron acquisition factors, lipopolysaccharides, polysaccharide capsules, and invasins, which are usually encoded on pathogenicity islands (PAIs), plasmids and other mobile genetic elements. Mechanisms underlying the dynamics of ExPEC transmission and the selection of virulent clones are still poorly understood and require further research. The time shift between colonization of ExPEC and the development of infection remains problematic in the context of establishing the relation between consumption of contaminated food and the appearance of first disease symptoms. What appears to be most difficult is to prove that ExPEC strains cause disease symptoms and to examine the mechanism of transition from the asymptomatic colonization of the intestines to the spreading of the bacteria outside the digestive system. A significant problem for researchers who are trying to ascribe ExPEC transmission to food, people or the environment is to draw the distinction between colonization of ExPEC and infection. Food safety is an important challenge for public health both at the production stage and in the course of its processing and distribution. Examination of the genetic similarity of ExPEC strains will allow to determine their origin from different sources. Many levels of genotyping have been proposed in which the typing of strains, plasmids and genes is compared in order to obtain a more complete picture of this complex problem. The aim of our study was to characterize E. coli strains isolated from humans, animals and food for the presence of bacterial genes encoding virulence factors such as toxins, and iron acquisition systems (siderophores) in the context of an increasing spread of ExPEC infections.

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Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture

Cited by 9 publications

References 33 publications

Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes

Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes

Role of CSE1034 in Escherichia coli Biofilm Destruction

Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports

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