Human Seminal Plasma Proteinase Inhibitor: Action Toward Some Trypsinlike Arginine Amidases from Humans

Hara, Ikuo; Matsuda, Yoshihisa; Kaneko, Shohei; Ishikawa, Hiromichi; Sato, Hiroo; Lee, H.-K.; Akihama, Sumiyuki

doi:10.3109/01485019308988376

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…HSP-TI inhibits human thrombin [8]. The present investigation confirmed that HSP-TI acts on the thrombin-like enzyme in human seminal plasma as well (Figure 4).…”

Section: Discussionsupporting

confidence: 70%

See 1 more Smart Citation

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

Park¹,

Yoshimura²,

Nozawa³

et al. 1997

Archives of Andrology

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“…HSP-TI inhibits human thrombin [8]. The present investigation confirmed that HSP-TI acts on the thrombin-like enzyme in human seminal plasma as well (Figure 4).…”

Section: Discussionsupporting

confidence: 70%

“…We could not evaluate the activity recovery of proteolytic enzymes, in particular thrombinlike enzyme, since seminal plasma contains proteinase inhibitors [3,8,191, precluding the measurement of the enzyme activity. HSP-TI inhibits human thrombin [8].…”

Section: Discussionmentioning

confidence: 99%

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

Park¹,

Yoshimura²,

Nozawa³

et al. 1997

Archives of Andrology

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“…Human semen has been shown to contain several proteinase inhibitors. [68][69][70] HSP-TI, which is known to inhibit human thrombin, 61 was found to have affinity to the thrombin-like enzyme in human seminal plasma. 7 Functionally active PCI is also detected in seminal plasma at very high concentrations-almost 30 to 40 times higher than those found in human blood.…”

Section: Proteinase Inhibitorsmentioning

confidence: 99%

Seminal Hemostatic Factors: Then and Now

Lwaleed¹,

Goyal²,

Delves³

et al. 2007

Semin Thromb Hemost

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Human semen spontaneously coagulates and subsequently liquefies following ejaculation. The exact reasons for this phenomenon are not entirely understood. Research has resulted in the discovery of several seminal proteins that are already known from another major domain of human physiology (i.e., blood coagulation and fibrinolysis). Some believe that regulation of seminal coagulation and fibrinolytic liquefaction may bear analogies to the well-described pathways operational in blood. We here attempt to summarize the subject, revisiting earlier findings and covering the more recent and extensive data on hemostatically functional seminal proteins, including the findings of our own group. Evidence for the existence of each key hemostatic factor in semen is presented along with any correlation with indices of male fertility. Several probably possess alternative functions including anti-inflammatory action, aiding sperm capacitation and classic hemostasis following intercourse-induced bleeding. Established mechanisms such as the high molecular weight seminal vesicle system are also considered as potentially interacting with conventional hemostatic pathways to regulate seminal coagulation and liquefaction. Although our grasp of this subject continues to evolve, there still remain many unanswered questions. A more complete understanding may one day prove useful in designing technologies for improved diagnostics in male infertility and potentially aiding assisted human reproductive therapies.

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Inhibitors on an Elastase-Like Enzyme Activity Catalyzing Suc-Ala-Ala-Pro-Leu-Pna Amidolysis in Human Seminal Plasma

Matsuda

Hara

Tomomasa

et al. 2000

Archives of Andrology

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The behavior of some proteinase inhibitors toward the Suc-Ala-Ala-Pro-Leu-pNA amidolytic enzyme activity in human seminal plasma (HSP) was tested. [(2S, 3R)-3-Amino-2-hydroxy-5-methyl-hexanoyl]L-valyl-L-valyl-L-aspartic acid (Amastatin) and 3-[1-[(2-(hydroxymethyl)- -pyrolidinyl)-2-methylpropyl]-carbamoyl] octanohydroxamic acid (Actinonin) showed strong inhibitory effects. No inhibition of this present enzyme activity was seen with anti-human serum (whole), anti-human leukocyte elastase, phenyl-methyl sulfonyl fluoride, Elastatinal, ethyeneglycol bis(beta-aminoethyl ethyl)N,N,N:N'-tetra acetic acid, and [L-3-trans-ethoxycarbonyl-oxirane-2-carbonyl]1-L-leucine(3-methylbutyl)a mido (E-64). No relation was observed between human pancreatic elastase antigen and the Suc-Ala-Ala-Pro-Leu-pNA amidolytic enzyme enzyme activity in HSP. Two peaks of Suc-Ala-Ala-Leu-Pro-pNA amidolytic enzyme activity were separated by Cellulofine GCL-2000 gel filtration and these activities were completely abolished by addition of Amastatin. Suc-Ala-Ala-Pro-Leu-pNA amidolytic enzyme activity in HSP is not an elastase-like metalloproteinase but is rather an acyl amidase-like leucine aminopeptidase.

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Human Seminal Plasma Proteinase Inhibitor: Action Toward Some Trypsinlike Arginine Amidases from Humans

Cited by 4 publications

References 13 publications

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

Seminal Hemostatic Factors: Then and Now

Inhibitors on an Elastase-Like Enzyme Activity Catalyzing Suc-Ala-Ala-Pro-Leu-Pna Amidolysis in Human Seminal Plasma

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