Human salivary anionic analysis using ion chromatography

Chen, Z.-F.; Darvell, B.W.; Leung, V.W.-H.

doi:10.1016/j.archoralbio.2004.05.002

Cited by 31 publications

(19 citation statements)

References 13 publications

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“…We also detected increased acetic acid concentrations in some of our patients with COPD. However like NH 4 ϩ , acetic acid is present in the saliva in much higher concentrations than those found in the EBC or plasma, and acetic acid represents the most abundant volatile anion in the saliva (23). This suggests that at least some of this acid may be derived from the saliva rather than the lungs.…”

Section: ) (B )mentioning

confidence: 88%

The Effects of Volatile Salivary Acids and Bases on Exhaled Breath Condensate pH

Effros

Casaburi

et al. 2006

Am J Respir Crit Care Med

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Rationale: Recent studies have reported acidification of exhaled breath condensate (EBC) in inflammatory lung diseases. This phenomenon, designated "acidopnea," has been attributed to airway inflammation. Objectives: To determine whether salivary acids and bases can influence EBC pH in chronic obstructive pulmonary disease (COPD). Methods: Measurements were made of pH, electrolytes, and volatile bases and acids in saliva and EBC equilibrated with air in 10 healthy subjects and 10 patients. In 2000, Hunt and colleagues reported that exhaled breath condensates (EBCs) are acidified during asthmatic exacerbations (1). They referred to this phenomenon as "acidopnea" and suggested that it reflected excess acid produced in the airways by inflammation. These observations were confirmed in studies of chronic obstructive pulmonary disease (COPD) and other inflammatory lung disorders (2-10). Subsequently, Hunt and colleagues found that EBC NH 4 ϩ concentrations were reduced in many patients with asthma (11). They postulated that production of NH 3 in the airways was reduced because of impaired glutaminase activity, and suggested that reductions in airway NH 3 production reduced local buffering, thereby promoting airway acidification. They also argued that concentrations of NH 4 ϩ in EBCs did not influence the pH of the EBC samples, which represented an accurate marker of airway acidification (12, 13). This study has analyzed the acid and base concentrations in saliva and EBC from 10 healthy subjects and 10 patients with COPD to determine whether EBC pH is influenced by volatile or nonvolatile constituents in the saliva. METHODSEleven healthy subjects and 10 subjects with COPD were initially selected but condensate from one healthy subject was deleted because high amylase concentrations indicated salivary contamination (Table 1). Spirometry was performed in all subjects (Sensormedics, Yorba Linda, CA). The patients had COPD (FEV 1 Ͻ 75% predicted, FEV 1 /FVC Ͻ 70%) (14) and an average smoking history of 59 Ϯ 43 (SD) pack-yr. None smoked within an hour of the study. All were taking prescribed maintenance bronchodilators, which were not used during the hour before collections.Patients exhaled for 1 h into an insulated 66-cm polycarbonate condenser cooled with recirculated ice water. One-way valves were used to ensure that subjects inhaled fresh air and exhaled into the condenser. Nose clips were not used. The mouthpiece and condenser were connected by a 450-ϫ 22-mm ID ventilator tubing (Corr-a-Flex 2; Hudson RCI, Temecula, CA) inclined upward to minimize salivary contamination. Condensate was collected using polycarbonate tubes. Collection and analysis of capillary plasma and saliva are described in the online supplement.All samples were stored at Ϫ80ЊC. Before analysis, all samples were allowed to thaw in room air for about 30 min. To minimize potential losses of volatile acids and bases, no attempt was made to remove ambient CO 2 from the EBC or saliva with inert gas (see the online supplement). The buffering capacity of th...

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Section: ) (B )mentioning

confidence: 88%

The Effects of Volatile Salivary Acids and Bases on Exhaled Breath Condensate pH

Effros

Casaburi

et al. 2006

Am J Respir Crit Care Med

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“…Until recently, acetic acid received little attention as an endogenous compound, but it has been known for some time to be present in exhaled breath condensate [24] and saliva [24,25]. Acetate, the anionic form of acetic acid in biological fluids, is a physiological metabolite involved in normal human biochemistry.…”

Section: Discussionmentioning

confidence: 99%

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Smith¹,

Sovová²,

Dryahina³

et al. 2016

J. Breath Res.

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A study has been carried out of the volatile organic compounds present in the exhaled breath of 58 cystic fibrosis (CF) patients. An important observation is that the acetic acid vapour concentration measured by selected ion flow tube mass spectrometry, SIFT-MS, is significantly elevated in the exhaled breath of CF patients, independent of the Pseudomonas aeruginosa (PA) infection status (PA-infected median 170 ppbv; PA-negative median 182 ppbv), compared to that of healthy controls (median 48 ppbv). The cause for this may be decreased pH of the mucus lining the CF airways. Thus, we speculate that non-invasive measurement of breath acetic acid concentration could serve as an indicator of the acidity of the CF airways mucosa.2

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“…Some methods have been reported for individual determination of thiocyanates in biological fluids, such as: capillary electrophoresis [5,10], capillary isotachophoresis [9], spectrophotometry [11,12], atomic absorption spectrometry [13], cathodic stripping voltammetry [14], ion chromatography [15][16][17][18][19]. Analyses of such samples characterized by complex composition of the matrix represent a difficult analytical task and require special sample pretreatment in order to obtain successful separation of analytes under investigation.…”

Section: Introductionmentioning

confidence: 99%

Application of ion chromatography for the determination of inorganic ions, especially thiocyanates in human saliva samples as biomarkers of environmental tobacco smoke exposure

Demkowska¹,

Polkowska²,

Namieśnik³

2008

Journal of Chromatography B

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Human salivary anionic analysis using ion chromatography

Cited by 31 publications

References 13 publications

The Effects of Volatile Salivary Acids and Bases on Exhaled Breath Condensate pH

The Effects of Volatile Salivary Acids and Bases on Exhaled Breath Condensate pH

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Application of ion chromatography for the determination of inorganic ions, especially thiocyanates in human saliva samples as biomarkers of environmental tobacco smoke exposure

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