Human Rotavirus Serotypes

Zissis, Georges; Lambert, J P; Kapsenberg, Jacoba G.; Enders, Gisela; Mutanda, L N

doi:10.1016/s0140-6736(81)91639-1

Cited by 22 publications

(16 citation statements)

References 12 publications

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“…The major neutralization antigen, VP7 protein, determines the G serotype. The most common serotypes in humans are G1, G2, G3 and G4 [55,56], but also serotypes G6 [15], G8 [30], G9 [35], G10 [2] and G12 [45] have been found. To date, at least 14 G serotypes have been detected in humans and animals [20].…”

Section: Introductionmentioning

confidence: 99%

Rotavirus serotypes and electropherotypes in Finland from 1986 to 1990

Maunula

Bonsdorff

1995

Archives of Virology

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Four epidemic seasons of rotaviruses were studied in Helsinki during 1986-1990. This is the first Scandinavian study, where both electropherotypes and serotypes are determined. Out of 5316 fecal specimens 769 (14.5%) rotavirus positive samples were detected by electron microscopy. Of these, 645 isolates (83.9%) gave a clear RNA pattern in gel electrophoresis and they clustered into 87 electropherotypes. An illustrative number of isolates representing each electropherotype (= E-type) was serotyped using VP7 protein-specific monoclonal antibodies for serotypes G1-G4 and without exceptions, within one E-type only a single serotype specificity was found. After establishment of the serotype of each E-type, the distribution of serotypes was scored as 61.2%, 2.0%, 0.5% and 29.8% for G1-G4, respectively; 6.5% remained untypable. Two seasons had one predominant E-type (Season 1, 1986-87, and Season 3, 1988-89, 84.2% and 80.6% of rotavirus positive samples, respectively). Both were followed by a season with no predominant E-type, but several minor E-types. Altogether, 5 short E-types (13/645 samples) with serotype G2 specificity were found, most of them occurring in Season 2. Only 2 E-types (3 samples) belonged to serotype G3. Group C rotavirus was found in 8 specimens. In this study a shift in serotypes, from G1 to G4, was observed in Finland in 1988/89; a similar shift was reported in many European countries at that time.

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Section: Introductionmentioning

confidence: 99%

Rotavirus serotypes and electropherotypes in Finland from 1986 to 1990

Maunula

Bonsdorff

1995

Archives of Virology

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“…Each species appears to have at least one unique serotype, and human rotaviruses have been divided into three serotypes (Woode et aL, 1976;Thouless et al, 1977Flewett et al, 1978;Yolken eta/., 1978;Zissis & Lambert, 1978Beards et al, 1980;Zissis et al, 1981). Recently, at least one new major group that does not react serologically with other rotavirus antisera has been described (Saif et al, 1980;McNulty et aL, 1981;Bridger et at., 1982).…”

Section: Introductionmentioning

confidence: 99%

Identification of a Neutralization-specific Antigen of a Calf Rotavirus

Killen

Dimmock

1982

Journal of General Virology

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SUMMARYMonospecific polyclonal antisera were raised in guinea-pigs against the calf rotavirus polypeptides VP 1, VP2, VP3* + 4*, VP4.2, VP6, VP7. I, VP7.2 and VP 10. All of the antisera gave a similar pattern of cytoplasmic immunofluorescence in rotavirus-infected cells, but spots of fluorescence of varying intensity with different sera were seen over the nucleus. Immune precipitation, using Staphylococcus aureus to collect immune complexes, showed that VP2 was precipitated by antiserum to VP2 (ct-VP2) and VP6 by ct-VP6, ct-VP7.1 and ct-VP7.2 both precipitated the same range of proteins from infected cells (VP7, VP7.1 and VP7.2) or from virions (VP7.1 and VP7.2). VP 10, either from virions or infected cells, was not precipitated by a-VP 10. The only antiserum which efficiently neutralized infectivity was a-VP7.2. There were low levels of neutralization with a-VP10 (but the results varied from experiment to experiment) and traces with a-VP6, ct-VP7.1 and the other antisera did not neutralize even though a-VP7.1 agglutinated double-shelled particles as seen in immune electron microscopy to a greater extent than ~-VP7.2. Both VP7.1 and VP7.2 were shown to be glycoproteins by tunicamycin treatment of infected cells. Core particles only were agglutinated by a-VP10. All the evidence leads us to conclude that there were major neutralizing antigenic determinants present on VP7.2, a minor component of the outer shell of the virion.

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“…Both single-and doubleshelled particles are seen in infected stool. The outer shell contains typespecific antigens, whereas the inner shell contains group-specific antigens (2,21).…”

Section: Rotavirusmentioning

confidence: 99%

“…There are two major serotypes of human rotavirus and an unknown number of less common human serotypes (7,20,21). Four major groups of rotavirus have been identified, represented by human, bovine, porcine, and canine-simian strains (7).…”

Section: Rotavirusmentioning

confidence: 99%