Human rhabdosphincter cell culture: A model for videomicroscopy of cell contractions

Abstract

These results demonstrate that cholinergic stimulation triggers contraction of cultured human rhabdosphincter cells. This model might help to understand external urethral sphincter physiology and to establish new therapies for the treatment of sphincter dysfunctions. Prostate 47:189-193, 2001.

Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence

“…Primary cell culture was established using the half‐dry method modified from a previously established protocol . RS cell culture is rare with only one publication in this area . Briefly, RS tissues were cut into 1 × 1 mm 2 pieces and placed into the bottom of 25 cm 2 flasks.…”

“…23 RS cell culture is rare with only one publication in this area. 24 Briefly, RS tissues were cut into 1 × 1 mm 2 pieces and placed into the bottom of 25 cm 2 flasks. Culture flasks containing 2 mL of medium were placed upside-down in a 5% CO 2 incubator at 37°C for 2 h to allow the tissues to attach.…”

Membrane properties of single muscle cells of the rhabdosphincter of the male urethra

“…Electrophysiological studies on human RS cells were performed in primary cell cultures. Biopsies were taken from the prostatic part of the RS [6,13]. Subsequently, the small amounts of striated muscle cells were collected under microscopic control.…”

“…In order to investigate the potential of this new therapy, it is necessary to analyze the cellular properties of myoblasts taken from the RS and skeletal muscle (SKM) in more detail. Corvin and coworkers reported [13] that striated muscle fibres that are capable to contract upon application of agonists can be obtained from explant cultures grown from prostatectomy and cystoprostatectomy specimens. Their data suggest that the human RS contains progenitor cells as other SKM (also called satellite cells, see Ref.…”

In vitro models: research in physiology and pharmacology of the lower urinary tract

“…Similarly, in the LUT, cultures of urethral, urinary bladder and ureter cells have been reported, but pharmacology remains largely uncharacterized (e.g. Baskin et al ., 1993; Kropp et al ., 1999; Corvin et al ., 2001). Organ bath studies of ureter, urethra and more common urinary bladder have been reported and the reader is referred to representative examples (Levin et al ., 1995; Pontari et al ., 2004; Su et al ., 2004; Wheeler et al ., 2005).…”