Human red blood cell behaviour in hydroxyethyl starch: probed by single cell spectroscopy

Microplastics are plastic particles less than 5 mm in size but larger than 0.1 µm. Widespread microplastic pollution in aquatic and terrestrial environment is raising serious concern globally due to its detrimental impacts on living beings. This urge for the necessity of a sensitive analytical tool, which can facilitate reliable identification of microplastics. Micro-Raman spectroscopy enables molecular level structural details of samples and thus highly preferable for the identification of microplastics in water. Besides being a non-destructive technique enabling fast analysis, this technique requires minimal/no sample preparation. The present work demonstrates the performance of a custom built Micro-Raman spectrometer for the discrimination of various microplastics in water based samples. Micro- Raman analysis have been found quite successful in the identification of Polystyrene (PS), Polyethylene terephthalate (PET), High Density Poly ethylene (HDPE), Low Density Poly ethylene (LDPE) etc. The Micro-Raman spectral data have been capable enough for the discrimination of high-density polyethylene (HDPE,> 0.940 g/cm3) and low-density polyethylene (LDPE,< 0.930 g/cm3) microplastics, especially due to the variations in the C-H stretching vibrations (2825-2970 cm−1). The ratio of intensities of asymmetric CH2 stretching mode (2879 cm−1) and the symmetric CH2 stretching mode (2847 cm−1) are found to be higher for HDPE with respect to LDPE. Moreover, intensity variations were also observed for the antisymmetric and symmetric C-C stretching bands present at 1059 cm−1 and 1126 cm−1 respectively. The band at 1167 cm−1 arising from CH vibration have also shown an intensity enhancement in HDPE samples.

show abstract

Recent advances in Raman spectroscopy for skin diagnosis

Wu¹,

Gao²,

Wei³

2023

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

The skin is the largest organ in humans. It comprises about 16% of our body. Many diseases originate from the skin, including acne vulgaris, skin cancer, fungal skin disease, etc. As a common skin cancer in China, melanoma alone grows at year rate of nearly 4%. Therefore, it is crucial to develop an objective, reliable, accurate, non-invasive, and easy-to-use diagnostic method for skin diseases to support clinical decision-making. Raman spectroscopy is a highly specific imaging technique, which is sensitive, even to the single-cell level in skin diagnosis. Raman spectroscopy provides a pattern of signals with narrow bandwidths, making it a common and essential tool for researching individual characteristics of skin cells. Raman spectroscopy already has a number of clinical applications, including in thyroid, cervical and colorectal cancer. This review will introduce the advantages and recent developments in Raman spectroscopy, before focusing on the advances in skin diagnosis, including the advantages, methods, results, analysis, and notifications. Finally, we discuss the current limitations and future progress of Raman spectroscopy in the context of skin diagnosis.

show abstract

Human red blood cell behaviour in hydroxyethyl starch: probed by single cell spectroscopy

Abstract: Hydroxyethyl starch (HES) is a commonly used intravenous fluid in hospital settings.

Cited by 4 publications

References 50 publications

Red blood cells under externally induced stressors probed by micro-Raman spectroscopy

Red blood cells under externally induced stressors probed by micro-Raman spectroscopy

Identification of Microplastics Using a Custom Built Micro-Raman Spectrometer

Recent advances in Raman spectroscopy for skin diagnosis

Contact Info

Product

Resources

About