Human reconstructed skin xenografts on mice to model skin physiology

Salgado, Giorgiana; Ng, Yi Zhen; Koh, Li Fang; Goh, Christabelle S M; Common, John E.A.

doi:10.1016/j.diff.2017.09.004

Cited by 35 publications

(28 citation statements)

References 116 publications

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“…For instance, similar to animal models for psoriasis, immune cells and peripheral blood (including PBMCs and stem cells) from patients with HS can be used to reconstitute immunodeficient mice. Healthy human skin can then be engrafted onto reconstituted mice, developing a ‘multi‐tissue humanized model’, permitting studies of complex multicellular pathways that may be missed with either immune cells or skin engraftment alone . It is also possible to use HS cell transfer combined with HS skin engraftments so that HS disease pathology is represented by both systemic and local components.…”

Section: Reportmentioning

confidence: 99%

“…fibrin or collagen). There are also reports using in vivo chamber housing with silicon chambers and a human cell mixture as a method of engraftment . These methods may provide more structural support for the xenograft, contribute to maintenance of the disease and lead to more experimental consistency.…”

Section: Reportmentioning

confidence: 99%

“…Healthy human skin can then be engrafted onto reconstituted mice, developing a 'multi-tissue humanized model', permitting studies of complex multicellular pathways that may be missed with either immune cells or skin engraftment alone. 10 It is also possible to use HS cell transfer combined with HS skin engraftments so that HS disease pathology is represented by both systemic and local components. Because the NSG-SGM3 mice in our study were essentially lacking certain immune cells, this may have led to the alteration of disease in the HS lesions.…”

Section: Reportmentioning

confidence: 99%

“…There are also reports using in vivo chamber housing with silicon chambers and a human cell mixture as a method of engraftment. 10 These methods may provide more structural support for the xenograft, contribute to maintenance of the disease and lead to more experimental consistency. Lastly, although NSG-SGM3 mice have been used extensively for xenograft models, immunodeficient mice, such as NOD.CgPrkdc scid Il2rg tm1Wjl (NSG), NODShi.Cg-Prkdc scid Il2rg tm1Sug (NOG), BALB/c-Rag2 null IL2rg null (BR2G) and others, 10 may be superior for HS xenografts.…”

Section: Reportmentioning

confidence: 99%

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Lessons learned from the development of a hidradenitis suppurativa xenograft mouse model

et al. 2019

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Summary Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease originating from the pilosebaceous unit, in which patients develop painful abscesses, sinus tracts, nodules and scarring, typically in intertriginous areas. Major gaps in our understanding of HS exist, and these may be partially due to the lack of an animal model for experimental studies. We developed an HS xenograft mouse model using human HS lesions grafted onto immunocompromised mice. Although the model had its limitations, several informative lessons were learned, which may contribute to future attempts at an HS animal model.

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Section: Reportmentioning

confidence: 99%

Section: Reportmentioning

confidence: 99%

Section: Reportmentioning

confidence: 99%

Section: Reportmentioning

confidence: 99%

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Lessons learned from the development of a hidradenitis suppurativa xenograft mouse model

et al. 2019

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“…Sci. 2018, 19, 3156 2 of 15 and physiology, 'humanized' mice models were generated [9]. Although humanized mice models have the ability to evaluate therapies of high clinical relevance, the major limitations in their use are the cost and complicated process to 'mass-produce' significant quantities of mice for wound-healing studies.…”

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confidence: 99%

525 PEG-Plasma Hydrogels Increase Epithelialization Using a Human Ex Vivo Skin Model

Stone

Wall

Florell

et al. 2018

Journal of Burn Care &Amp; Research

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In vitro cell culture methods are used extensively to study cellular migration, proliferation, and differentiation, which play major roles in wound healing but the results often do not translate to the in vivo environment. One alternative would be to establish an ex vivo model utilizing human discarded skin to evaluate therapies in a more natural setting. The purpose of this study was to institute such a model by creating 'wounds' in the center of a piece of discarded skin and treating them with three different biomaterials: collagen, polyethylene glycol (PEG)-fibrin, or PEG-platelet free plasma (PFP). Explants were cultured for 14 days with supernatant and microscopy images collected every 3 days to assess cytotoxicity and epithelialization. After 14 days, the explants were fixed, sectioned, and stained for cytokeratin-10 (CK-10), alpha-smooth muscle actin (α-SMA), and wheat germ (WG). Compared to controls, similar levels of cytotoxicity were detected for 12 days which decreased slightly at day 14. The PEG-PFP hydrogel-treated wounds epithelialized faster than other treatments at days 6 to 14. A 6-8 cell layer thick CK-10+ stratified epidermis had developed over the PEG-PFP hydrogel and cells co-stained by WG and α-SMA were observed within the hydrogel. An ex vivo model was established that can be used practically to screen different therapies exploring wound healing. Int. J. Mol. Sci. 2018, 19, 3156 2 of 15 and physiology, 'humanized' mice models were generated [9]. Although humanized mice models have the ability to evaluate therapies of high clinical relevance, the major limitations in their use are the cost and complicated process to 'mass-produce' significant quantities of mice for wound-healing studies. Alternatively, human skin samples that are usually discarded after an elective surgical procedure, such as abdominoplasty, may provide an ideal ex vivo platform to conduct high-throughput wound healing studies in the laboratory [7]. When a skin sample is freshly collected, most of the cells are viable and still can respond to an exogenous physiological insult, i.e., a full-thickness excision wound [10][11][12]. Whereas, ex vivo excisions created on a skin sample possess the key cells like keratinocytes, immune cells, fibroblasts, endothelial cells, and smooth muscle cells capable of migrating within the wound bed. Therefore, ex vivo models may be a good 'high-throughput' platform to evaluate materials or products that are intended to promote wound healing.Most of the regenerative skin products on the market are acellular skin substitutes with very few products incorporating therapeutic agents or cells to improve wound healing [13][14][15]. One such line of materials that are currently under investigation are hydrogels. Hydrogels are advantageous in both providing a moist wound environment, as well as acting as a conducive platform for skin cells to migrate and proliferate [16]. Natural biological polymers, such as collagen-, fibrin-, and glycosaminoglycan-based hydrogels have precedence due to their excel...

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