Human reaming debris: a source of multipotent stem cells

Wenisch, Sabine; Trinkaus, Katja; Hild, Anne; Hose, Dirk; Herde, Katja; Heiß, Christian; Kilián, O.; Alt, Volker; Schnettler, Reinhard

doi:10.1016/j.bone.2004.09.019

Cited by 140 publications

(90 citation statements)

References 53 publications

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“…The use of reaming debris as a source of autologous bone graft is further supported by a number of studies showing that bone material collected from reaming retains osteocyte viability as well as the presence of biochemical proteins known to stimulate bonehealing. 14,15,17,21 The intramedullary reaming debris has an abundance of progenitor cells and growth factors. [21][22][23][24] Various papers establish that the benefit from reamed particles is the existence of viable osteoprogenitors within the bone matrix itself, not merely in the marrow, which is well accepted.…”

Section: Discussionmentioning

confidence: 99%

“…14,15,17,21 The intramedullary reaming debris has an abundance of progenitor cells and growth factors. [21][22][23][24] Various papers establish that the benefit from reamed particles is the existence of viable osteoprogenitors within the bone matrix itself, not merely in the marrow, which is well accepted. 15,21,24,25 Quantitative analysis of the reaming debris have found higher levels of fibroblast growth factor (FGFa), platelet-derived growth factor (PDGF), insulin like growth factor (IGF)-1, BMP-2, and transforming growth factor (TGF)-b1 which play a vital role in osteosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…[21][22][23][24] Various papers establish that the benefit from reamed particles is the existence of viable osteoprogenitors within the bone matrix itself, not merely in the marrow, which is well accepted. 15,21,24,25 Quantitative analysis of the reaming debris have found higher levels of fibroblast growth factor (FGFa), platelet-derived growth factor (PDGF), insulin like growth factor (IGF)-1, BMP-2, and transforming growth factor (TGF)-b1 which play a vital role in osteosynthesis. 16 Wenisch and colleagues 21 studied human reaming debris using cell cultures and found multipotent stem cells capable of differentiating along the osteogenic pathway.…”

Section: Discussionmentioning

confidence: 99%

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Case Series of Femoral Shaft Fractures Innovatively Managed by Using Reamed Material as Internal Bone Graft for Early Fracture Union

Palanimalai¹,

Kolundan²,

Srinivasan³

et al. 2017

jemds

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BACKGROUNDFemur fracture treatment has constantly evolved over time from various conservative methods to various newer modalities of fixation. Since the incidence of malunion and non-union in conservative methods was more, gradually the treatment shifted to fixation as the primary mode of treatment. After IM nailing was introduced it has almost become a gold standard for femur fracture fixation. Reaming femoral canal is an integral part of nailing procedure. Previously, reamed material which stuck to the reamers were often ignored and neglected. Hence, recent interest has been in the judicial use of reamed material obtained through reaming. We thought if we could accumulate the reamed material in the fracture site, even with closed nailing, we could achieve the objective of using this potential bone graft material effectively. At this juncture, we have devised this new idea of reaming the proximal femoral canal before reducing the fracture, thereby depositing the reamed material in the fracture site and studied the effect. MATERIALS AND METHODSThis is a case series study carried out in KAP Viswanatham Government Medical College/MGM Government Hospital, Trichy from 2013 to 2016 of 40 cases of femoral shaft fractures satisfying inclusion/exclusion criteria. Entry portal created as in any other standard nailing procedure and after entry portal is made, we did not pass the guide wire into the distal fragment and did not reduce the fracture. Instead, the fracture was left as it is and the guide wire was passed up to the fracture site only. Instead of using routine cutting reamers, we used a slightly modified reamer. Serial reaming was done until the fracture site was reached and up to the maximum size. Then as per standard IM nailing technique, appropriate-sized nail was inserted and locked. RESULTSBridging bony callus was visible in 4 -6 weeks in 22 cases of which 17 were simple and 5 comminuted. They subsequently united radiologically in 8 -10 weeks and complete union seen in 10 -12 weeks. In the rest 18 cases, bony callus was visible in 6 -8 weeks. These 18 patients had radiological union in 10 -12 weeks and complete union in 12 -14 weeks. In our study, all 40 cases went for complete union. CONCLUSIONIn our study, all 40 cases united well clinically and radiologically. And we also noted that there was early union occurring in all 40 cases. There was no incidence of non-union in our study. We advocate this simple modification in reamer and the reaming technique to obtain early union in femoral shaft fractures. Hence, we conclude this is a useful and safe mode of fixing femoral shaft fractures to avoid potential second surgery for grafting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Case Series of Femoral Shaft Fractures Innovatively Managed by Using Reamed Material as Internal Bone Graft for Early Fracture Union

Palanimalai¹,

Kolundan²,

Srinivasan³

et al. 2017

jemds

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“…As established previously, 19,20 human mesenchymal stem cells (hMSCs) were isolated from bone reaming debris of five patients of various age, gender and without any particular clinical condition (Table S1), in order to have a statistically representative human sample. The study design was approved by the Local Ethics Commission (Reference number: 245/13) and all donors of reaming debris provided informed consent.…”

Section: Cell Culturesmentioning

confidence: 99%

Enhanced osteogenesis on titanium implants by UVB photofunctionalization of hydrothermally grown TiO₂ coatings

et al. 2015

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Even though Ti-based implants are the most used materials for hard tissue replacement, they may present lack of osseointegration on the long term, due to their inertness. Hydrothermal treatment (HT) is a useful technique for the synthesis of firmly attached, highly crystalline coatings made of anatase titanium dioxide (TiO 2 ), providing favorable nanoroughness and higher exposed surface area, as well as greater hydrophilicity, compared to the native amorphous oxide on pristine titanium. The hydrophilicity drops even more by photofunctionalization of the nanostructured TiO 2 -anatase coatings under UV light. Human mesenchymal stem cells exhibited a good response to the combination of the positive surface characteristics, especially in respect to the UVB pre-irradiation. The results showed that the cells were not harmed in terms of viability; even more, they were encouraged to differentiate in osteoblasts and to become osteogenically active, as confirmed by the calcium ion uptake and the formation of well-mineralized, bone-like nodule structures. In addition, the enrichment of hydroxyl groups on the HT-surfaces by UVB photofunctionalization accelerated the cell differentiation process and greatly improved the osteogenesis in comparison with the nonirradiated samples. The optimal surface characteristics of the HT-anatase coatings as well as the high potentiality of the photo-induced hydrophilicity, which was reached during a relatively short pre-irradiation time (5 h) with UVB light, can be correlated with better osseointegration ability in vivo; among the samples, the superior biological behavior of the roughest and most hydrophilic HT coating makes it a good candidate for further studies and applications.

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“…However, this study presents a bias as there are more reaming procedures in the group showing the best results (Table 7). Reaming debris is "a source of multipotent stem cells" [36] and "...a rich source of growth factors" [37,38]. This may explain the differences observed.…”

Section: Clinical Use Of Bmpmentioning

confidence: 99%

Growth factors in orthopaedic surgery: demineralized bone matrix versus recombinant bone morphogenetic proteins

Hinsenkamp

Collard

2014

International Orthopaedics (SICOT)

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During recent decades the utilisation of growth factors, especially BMPs, has received an increasing interest in orthopaedic surgery. For clinical implantation the two main options are demineralised bone matrix (DBM) and recombinant bone morphogenetic proteins (rhBMP). Many clinical studies agree on an equivalent osteoinductive effect between DBM, BMPs and autologous bone graft; however, the different origins and processing of DBM and rhBMP may introduce some fluctuations. Their respective characteristics are reviewed and possible interactions with their effectiveness are analysed. The main difference concerns the concentration of BMPs, which varies to an order of magnitude of 106 between DBM and rhBMPs. This may explain the variability in efficiency of some products and the adverse effects. Currently, considering osteoinductive properties, safety and availability, the DBM seems to offer several advantages. However, if DBM and rhBMPs are useful in some indications, their effectiveness and safety can be improved and more evidence-based studies are needed to better define the indications.

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Human reaming debris: a source of multipotent stem cells

Cited by 140 publications

References 53 publications

Case Series of Femoral Shaft Fractures Innovatively Managed by Using Reamed Material as Internal Bone Graft for Early Fracture Union

Case Series of Femoral Shaft Fractures Innovatively Managed by Using Reamed Material as Internal Bone Graft for Early Fracture Union

Enhanced osteogenesis on titanium implants by UVB photofunctionalization of hydrothermally grown TiO₂ coatings

Growth factors in orthopaedic surgery: demineralized bone matrix versus recombinant bone morphogenetic proteins

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Human reaming debris: a source of multipotent stem cells

Cited by 140 publications

References 53 publications

Case Series of Femoral Shaft Fractures Innovatively Managed by Using Reamed Material as Internal Bone Graft for Early Fracture Union

Case Series of Femoral Shaft Fractures Innovatively Managed by Using Reamed Material as Internal Bone Graft for Early Fracture Union

Enhanced osteogenesis on titanium implants by UVB photofunctionalization of hydrothermally grown TiO2 coatings

Growth factors in orthopaedic surgery: demineralized bone matrix versus recombinant bone morphogenetic proteins

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Product

Resources

About

Enhanced osteogenesis on titanium implants by UVB photofunctionalization of hydrothermally grown TiO₂ coatings