Human Pythiosis, Brazil

Bosco, Sandra de Moraes Gimenes; Bagagli, Eduardo; Araújo, João Pessoa; Candeias, J. A. N.; Franco, Marcello; Marques, Mariângela Esther Alencar; Mendoza, Leonel; Camargo, Rosangela Pires de; Marques, Sílvio Alencar

doi:10.3201/eid1105.040943

Cited by 64 publications

(32 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pythium insidiosum de Cock, Mendoza, Padhye, Ajello & Kaufman se destaca por ser uma espécie extremamente patogênica em eqüinos, bovinos, caprinos, cães, gatos e para o homem (Alexopoulos et al 1996), cuja ocorrência também é registrada no Brasil (Santurio et al 1998, Leal et al 2001, Sallis et al 2003, Rech et al 2004, Bosco et al 2005. Por outro lado, Pythium oligandrum Drechsler atua como hiperparasita de outros fungos, possuindo um importante papel no controle biológico (Alexopoulos et al 1996).…”

Section: Introductionunclassified

O gênero Pythium no Parque Estadual da Serra da Cantareira, estado de São Paulo, Brasil

Miranda

Zottarelli

2008

Hoehnea

View full text Add to dashboard Cite

Durante o período de junho de 2005 a junho de 2006 foram coletadas trimestralmente, no Parque Estadual da Serra da Cantareira, amostras de água e solo para o levantamento das espécies do gênero Pythium. As amostras coletadas foram iscadas, em laboratório, com substratos celulósicos, quitinosos e queratinosos. Foram identificadas seis espécies, as quais são primeira citação para o Parque: Pythium dissotocum Drechsler, P. echinulatum Matthews, P. middletonii Sparrow, P. rostratum Butler, P. torulosum Coker & Patterson e P. vexans de Bary.

show abstract

Section: Introductionunclassified

O gênero Pythium no Parque Estadual da Serra da Cantareira, estado de São Paulo, Brasil

Miranda

Zottarelli

2008

Hoehnea

View full text Add to dashboard Cite

show abstract

“…However, these techniques display variable sensitivity and specificity, especially during early infection when the immune response is not fully mounted (8). Definitive diagnosis can be achieved using molecular methods, including species-specific nested PCR (3) or DNA sequencing of variable regions (1,2).…”

Section: Case Reportmentioning

confidence: 99%

Molecular Diagnosis of Subcutaneous Pythium insidiosum Infection by Use of PCR Screening and DNA Sequencing

et al. 2012

Self Cite

View full text Add to dashboard Cite

h Pythium insidiosum is an emerging human pathogen classified among brown algae and diatoms that can cause significant morbidity and mortality in otherwise healthy individuals. Here we describe a pediatric patient with pythiosis acquired in the southern United States, diagnosed by molecular screening and DNA sequencing of internal transcribed spacer region 1. CASE REPORTA n otherwise healthy 14-year-old girl living in urban north Texas presented to an urgent care facility with a 2-week history of a progressively enlarging, erythematous bump on the medial aspect of her left lower leg. She denied recent travel but was noted to have gone swimming in a swimming pool with signs of algal overgrowth. She was treated with incision and drainage and given a course of oral cephalexin. Although initially responding to antibiotics, over 2 weeks she again developed worsening local edema and erythema. The patient was switched to oral clindamycin but did not show improvement, prompting hospital admission about 3 weeks into her illness. She was administered intravenous clindamycin, and an ultrasound of the area revealed soft-tissue swelling with some patchy areas suggestive of fluid accumulation but without clear signs of an abscess. Incision and drainage was performed, and a drain was placed. Wound cultures were sent and returned positive for Staphylococcus intermedius and Pseudomonas aeruginosa. Based on the culture sensitivities of those organisms, antibiotic therapy was tailored to cefepime, tobramycin, and clindamycin. The patient showed clinical improvement and was discharged on oral ciprofloxacin 1 week after admission.After approximately 5 days, erythema and edema around the wound had visibly increased and the patient was readmitted approximately 1 month after onset of her initial symptoms. She was administered intravenous meropenem and amikacin and incision and drainage was repeated, revealing an abscess measuring 10 cm by 15 cm. A drain was again placed, and wound cultures were sent for growth of bacteria, fungi, and acid-fast bacilli. Histologic sections from debridement material demonstrated an acute and chronic inflammatory infiltrate, interrupted by eosinophilic pools comprised of poorly formed granulomas with central necrosis admixed with neutrophils, eosinophils, and mineralized debris (Fig. 1A). Rare hyphal elements on Gomori methenamine silver stain were identified (Fig. 1B and C). Consequently, amikacin was discontinued, and the patient was started on liposomal amphotericin B (250 mg intravenously [i.v.]

show abstract

“…2 Human pythiosis cases occur worldwide but are probably underrecognized and thus, misdiagnosed. 7,8 We report a case of contact lens-related P. insidiosum keratitis imported in France after traveling to Thailand, in which the strain was identified by nucleic acid sequencing and the patient was cured with a large therapeutic penetrating keratoplasty.…”

Section: Introductionmentioning

confidence: 99%

Imported Pythium insidiosum Keratitis After a Swim in Thailand by a Contact Lens-Wearing Traveler

Lelièvre¹,

Borderie²,

García-Hermoso³

et al. 2015

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract.A 30-year-old woman with a history of contact lens wear and exposure to swimming pool water in Thailand presented with a non-responsive, progressive corneal ulcer of the right eye. Confocal microscopy evidenced septate linear branching structures, raising suspicion of fungal keratitis. She was promptly treated with topical antibiotics and both topical and intravenous caspofungin plus voriconazole. Worsening of the clinical picture after 1 month of intensive medical therapy led to a large therapeutic penetrating keratoplasty being performed. Corneal cultures grew a mold-like organism, which was identified by sequencing as Pythium insidiosum, an aquatic oomycete. After 4 years of follow-up, the graft exhibits no infection relapse, but graft transparency has been lost after two rejection episodes. Keratoplasty combined with antifungal treatment may offer a cure to P. insidiosum keratitis, although long-term preservation of corneal transparency is difficult to obtain.

show abstract

Human Pythiosis, Brazil

Cited by 64 publications

References 14 publications

O gênero Pythium no Parque Estadual da Serra da Cantareira, estado de São Paulo, Brasil

O gênero Pythium no Parque Estadual da Serra da Cantareira, estado de São Paulo, Brasil

Molecular Diagnosis of Subcutaneous Pythium insidiosum Infection by Use of PCR Screening and DNA Sequencing

Imported Pythium insidiosum Keratitis After a Swim in Thailand by a Contact Lens-Wearing Traveler

Contact Info

Product

Resources

About