Human primed and naïve PSCs are both able to differentiate into trophoblast stem cells

Viukov, Sergey; Shani, Tom; Bayerl, Jonathan; Aguilera-Castrejon, Alejandro; Oldak, Bernardo; Sheban, Daoud; Tarazi, Shadi; Stelzer, Yonatan; Hanna, Jacob; Novershtern, Noa

doi:10.1016/j.stemcr.2022.09.008

Cited by 24 publications

(26 citation statements)

References 37 publications

(100 reference statements)

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“…During the revision of our manuscript, a study from Arima laboratory reported that activation of the imprinted microRNA cluster C19MC occurs in naive‐TSCs but not in TSCs converted from conventional PSCs through BMP stimulation, and it confers differentiation potential into trophoblast lineages (Kobayashi et al , 2022 ). It will be interesting in future to systematically compare the epigenome and the expression of imprinted genes of TSCs obtained through different protocols, like differentiation of naive PSCs (Dong et al , 2020 ; Guo et al , 2021 ; Io et al , 2021 ), conversion of conventional PSCs with (Soncin et al , 2018 ; Wei et al , 2021 ; Jang et al , 2022 ; Kobayashi et al , 2022 ) or without BMP stimulation (this study and Viukov et al , 2022 ) or somatic cell reprogramming (Liu et al , 2020 ), and to functionally test the consequences of altered imprinted gene expression.…”

Section: Discussionmentioning

confidence: 91%

“…While we were finalising our manuscript, a study from the Hanna laboratory reported the generation of TSCs at high efficiency from conventional PSCs using a combination of TGF‐beta receptor inhibitor and Vitamin C in N2B27 medium (Viukov et al , 2022 ). Interestingly, no induction of naive genes was detected, indicating the existence of multiple trajectories followed by conventional PSCs towards a TSC state.…”

Section: Discussionmentioning

confidence: 99%

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Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation

et al. 2023

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In human embryos, naive pluripotent cells of the inner cell mass (ICM) generate epiblast, primitive endoderm and trophectoderm (TE) lineages, whence trophoblast cells derive. In vitro, naive pluripotent stem cells (PSCs) retain this potential and efficiently generate trophoblast stem cells (TSCs), while conventional PSCs form TSCs at low efficiency. Transient histone deacetylase and MEK inhibition combined with LIF stimulation is used to chemically reset conventional to naive PSCs. Here, we report that chemical resetting induces the expression of both naive and TSC markers and of placental imprinted genes. A modified chemical resetting protocol allows for the fast and efficient conversion of conventional PSCs into TSCs, entailing shutdown of pluripotency genes and full activation of the trophoblast master regulators, without induction of amnion markers. Chemical resetting generates a plastic intermediate state, characterised by co‐expression of naive and TSC markers, after which cells steer towards one of the two fates in response to the signalling environment. The efficiency and rapidity of our system will be useful to study cell fate transitions and to generate models of placental disorders.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation

et al. 2023

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“…Notably, stem cell-based hTSC has been widely proposed in the later years, not only using naïve pluripotent stem cell, which needs more complex cultivation conditions and cell culture technical level, but also using primed stem cell, which possesses more commercially serum-free medium [36,41,[103][104][105]. BMP4 is reported could be able to transform primed stem cells into hTSC, and the potential function of BMP4 in pluripotency is reviewed recently [106].…”

Section: Application Of Stem Cells In Trophoblast Lineagementioning

confidence: 99%

Early differentiation and gene expression characteristics of trophoblast lineages

Qin

et al. 2023

Biology of Reproduction

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With the development of the embryo, the totipotent blastomere undergoes the first lineage decision to the inner cell mass (ICM) and the trophectoderm (TE). The ICM forms the fetus while the TE forms the placenta, which is one of the most unique organs in mammals serving as the interface between maternal and fetal. Proper trophoblast lineage differentiation is crucial for correct placental and fetal development, including the TE progenitor self-renewal and its differentiation toward mononuclear cytotrophoblast (CTB), which later either develops into invasive extravillous trophoblast (EVT), remodeling the uterine vascular, or fuses into multinuclear syncytiotrophoblast (STB), secreting pregnancy-sustaining hormone. Aberrant differentiation and gene expression of trophoblast lineage is associated with severe pregnancy disorders and fetal growth restriction. This review focuses on the early differentiation and key regulatory factors of trophoblast lineage, which have been poorly elucidated. Meanwhile, the recent development of trophoblast stem cells (TSC), trophectoderm stem cells (TESC), and blastoids derived from pluripotent stem cells bring the accessible model to investigate the profound mystery of embryo implantation and placentation were also summarized.

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“…Optionally, PSCs may be able to differentiate into germ line cells [ 65 , 66 ]. Additionally, human PSCs are readily differentiated into trophoblast [ 67 ].…”

Section: Differentiation Of Pscsmentioning

confidence: 99%

Pluripotent Stem Cells of Order Carnivora: Technical Perspective

Мензоров

2023

IJMS

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Human and mouse induced pluripotent stem cells (PSCs) are widely used for studying early embryonic development and for modeling of human diseases. Derivation and studying of PSCs from model organisms beyond commonly used mice and rats may provide new insights into the modeling and treating human diseases. The order Carnivora representatives possess unique features and are already used for modeling human-related traits. This review focuses on the technical aspects of derivation of the Carnivora species PSCs as well as their characterization. Current data on dog, feline, ferret, and American mink PSCs are summarized.

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Human primed and naïve PSCs are both able to differentiate into trophoblast stem cells

Cited by 24 publications

References 37 publications

Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation

Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation

Early differentiation and gene expression characteristics of trophoblast lineages

Pluripotent Stem Cells of Order Carnivora: Technical Perspective

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