Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo

Heimburg, Dennis von; Zachariah, Sascha; Kühling, Hendrik; Heschel, I.; Schoof, H.; Hafemann, B.; Pallua, Norbert

doi:10.1016/s0142-9612(00)00186-1

Cited by 219 publications

(129 citation statements)

References 23 publications

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“…HYAFF Ò 11 is based on hyaluronic acid modified by esterification which increases the hydrophobicity, the residence time in vivo and makes it more resistant to hyaluronidase activity. Previous studies observed the potential to support expansion and differentiation of adipose precursor cells in vivo [8]. When implanted in nude mice human preadipocyte-seeded HYAFF Ò 11 sponges showed a higher cell density than collagen constructs after 3 weeks [10].…”

Section: Introductionmentioning

confidence: 83%

“…The three-dimensional geometry of the native ECM constrains cells during regeneration and provides space for tissue development [25] and in vitro studies have shown that the scaffold geometry promotes preadipocyte differentiation [31]. The architecture of adipose tissue has been reconstructed using adipose-derived stromal cells seeded onto different scaffold materials [6][7][8]13,14]. Hyaluronic acid-based scaffolds have been used clinically to successfully direct chondrocyte development [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

“…Materials that meet fundamental requirements such as controlled degradation, cytotoxicity and immunogenity are needed. Research reports have shown successful and reproducible inoculation and culturing of adipocyteand preadipocyte-precursor cells (preadipocytes and progenitors, respectively) on synthetic [6,7] or natural [8] prefabricated scaffolds [9] with subsequent differentiation in vitro or adipose tissue formation in vivo [10]. The scaffolds within these bio-hybrids provided the seeded cells specific attachment or binding sites, shaped the tissue construct and functioned as geometrical environments in which not only cells received their essential cues for a structural cell organization and cellular behaviour but also cellular function was modulated [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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a b s t r a c tHistioconductive approaches to soft-tissue defects use scaffolds seeded with lineage-and tissue-specific progenitors to generate tissue which should reside in equilibrium with adjacent tissue. Scaffolds guide histiogenesis by ensuring cell-cell and cell-matrix interactions. Hyaluronic acid-based (HA) preadipocyte-seeded scaffolds were evaluated for their adipo-conductive potential and efficacy in humans. Preadipocytes were isolated from lipoaspirate material and seeded on HA scaffolds. The cellular biohybrid (ADIPOGRAFT Ò ) and an acellular control scaffold (HYAFF Ò 11) were implanted subcutaneously. At specific time points (2, 8 and 16 weeks) explants were analyzed histopathologically with immunohistochemistry. No adverse tissue effects occurred. Volume loss and consistent degradation of the HYAFF Ò 11 scaffolds compared to the ADIPOGRAFT Ò group indicated progressive tissue integration. No consistent histological differences between both groups were observed. By 8 weeks all void spaces within the scaffolds were filled with cells with pronounced matrix deposition in the ADIPOGRAFT Ò bio-hybrids. Here we show that HA scaffolds were stable cell carriers and had the potential to generate volumeretaining tissue. However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.

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Section: Introductionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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“…Structural biopolymer carriers such as sponges or nonwoven meshes provide a permissive microenvironment in which seeded preadipocytes proliferate and differentiate. In the absence of seeded cells, common scaffold materials alone have not been demonstrated as having appreciable adipogenic activity [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Polylactide glycolic acid (PLGA), polyglycolic acid (PGA), hyaluronan, and collagen-I in the form of nonwoven meshes or sponges have been evaluated as three-dimensional carriers for ex vivo expanded preadipocytes with varying success [13][14][15][16]. The in vivo efficacy of constructs in generating mature adipose tissue has been clearly correlated to the in vitro cell inoculation of the scaffolds and on the structure and type of biopolymer used [17].…”

Section: Introductionmentioning

confidence: 99%

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

Hemmrich¹,

Sijpe²,

Rhodes³

et al. 2008

Journal of Surgical Research

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Background. There is a major clinical need for strategies for adequately reconstructing the soft tissue defects found after deep burns, tumor resection, or trauma. A promising solution is adipose tissue engineering with preadipocytes, stem-cell derived precursors of the adipose tissue, implanted within biomaterials. This pilot study evaluated hyaluronan gels mixed with autologous undifferentiated preadipocytes in a pig model for their potency to generate new fat.Materials and methods. Preadipocytes were isolated from intra-abdominal pig fat by collagenase digestion, plated on fibronectin-coated culture dishes in Dulbecco's Modified Eagle Medium/Ham's F12 (Biochrom, Berlin, Germany) combined with 10% pig serum, expanded, and mixed with hyaluronan gel. Two types of gels with varying degrees of amidation of the carboxyl groups were tested (HYADD3, HYADD4). Cell-loaded gels and unseeded controls were injected subcutaneously into the ears of three pigs, explanted at 6 wk, and analyzed histologically.Results. Both cell-loaded specimens were detected macroscopically. They demonstrated a slight volume effect with limited stability after 6 wk. Unloaded HYADD3 and HYADD4 controls could not be identified at the time of explantation. Histology of HYADD3 revealed islets of mature adipocytes and vessels embedded in fat tissue surrounded by gel. In contrast, no fat formation was found in HYADD4 gels when implanted in the ear.Conclusions. Histological findings demonstrate that HYADD3 is a promising gel for generating adipose tissue. Even though HYADD3 might be a potential material for the reconstruction of small tissue defects, the question remains as to whether the adipose tissue within the gel is attributable to preadipocyte maturation or ingrowth from neighboring tissue.

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Characterization and Culturing of Adipose‐Derived Precursor Cells

Hutmacher¹,

Reichert²,

Olkowska‐Truchanowicz³

et al. 2009

Emerging Technology Platforms for Stem Cells

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Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo

Cited by 219 publications

References 23 publications

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

Characterization and Culturing of Adipose‐Derived Precursor Cells

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