Human polyomavirus JC presence in chronic inflammatory rheumatic diseases patients treated with anti-TNF-α: Evaluation of JC viral loads in urine and plasma samples

Bellizzi, Anna; Mischitelli, Monica; Anzivino, Elena; Rodio, Donatella Maria; Scribano, Daniela; Cacciotti, F.; Cioccolo, Sara; Delbue, Serena; Valesini, Guido; Pietropaolo, Valeria

doi:10.1016/j.jbspin.2014.12.010

Cited by 3 publications

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“…Regarding viremia, it was mainly associated to viruria during the entire follow-up (Figure 1 ). Although JC viremia seems to be essential for the development of PML and it was detected in 37.5% (12/32) of our patients at T18, its short temporality precludes its usefulness in screening or diagnostic algorithms, as already demonstrated by other Authors in comparable studies (Rinaldi et al, 2010 ; Bellizzi et al, 2015 ; Verheyen et al, 2015 ). Hence, monitoring urine JCPyV replication is a good, non-invasive method to check viral pathogenic potential.…”

Section: Discussionmentioning

confidence: 52%

Increased Prevalence of Human Polyomavirus JC Viruria in Chronic Inflammatory Rheumatic Diseases Patients in Treatment with Anti-TNF α: A 18 Month Follow-Up Study

et al. 2016

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Chronic inflammatory rheumatic diseases (CIRDs) are immune-mediated pathologies involving joints. To date, TNFα-blocking agents administration is the most promising therapy, although these treatments are associated with an increased Polyomavirus JC (JCPyV) reactivation, the etiological agent of the Progressive Multifocal Leukoencephalopathy (PML). The aim of this study was the recruitment and the analysis of a CIRDs cohort in order to investigate a possible correlation between JCPyV presence and the influence of anti-TNF-α agents on viral loads. Blood and urine samples were collected from 34 CIRDs subjects prior the first anti-TNF-α infusion (T0) and after 3 (T3), 6 (T6), 12 (T12), and 18 (T18) months. Results showed persistent JC viruria significantly higher than JC viremia throughout the 18 month follow-up study (p = 0.002). In JCPyV positive samples, the non-coding control region (NCCR) was analyzed. Results evidenced archetypal structures (type II-S) in all isolates with the exception of a sequence isolated from a plasma sample, that corresponds to the type II-R found in PML subjects. Finally, the viral protein 1 (VP1) genotyping was performed and results showed the prevalence of the European genotypes 1A, 1B, and 4. Since only few studies have been carried out to understand whether there is a PML risk in CIRDs population infected by JCPyV, this study contributes to enrich literature insight on JCPyV biology in this cluster. Further investigations are necessary in order to recognize the real impact of biologics on JCPyV life cycle and to identify possible and specific viral variants related to increased virulence in CIRDs patients.

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Section: Discussionmentioning

confidence: 52%