Human pleural B-cells regulate IFN-γ production by local T-cells and NK cells in a<i>Mycobacterium tuberculosis</i>-induced delayed hypersensitivity reaction

Schierloh, Pablo; Landoni, Verónica Inés; Balboa, Luciana; Musella, Rosa M.; Castagnino, Jorge; Moraña, Eduardo José; Casado, Graciela C. de; Palmero, Domingo; Sasiain, María C.

doi:10.1042/cs20130769

Cited by 21 publications

(23 citation statements)

References 40 publications

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“…To make a wide characterization of blood B‐cell subsets circulating in ANDV + patients, we performed immunophenotyping based on CD27/IgD expression among CD19 + CD3 neg gated lymphocytes/lymphoblasts . The percentages of naive B cells – defined by surface IgD expression and lack of CD27 – were strongly reduced in ANDV + patients when compared with HS or with ARS patients (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For experiments described in Fig. , a group of patients with pulmonary tuberculosis (TB; n = 10; mean age 31 years, range 19–43 years) were enrolled and diagnosed at the Muñiz Hospital, as previously described …”

Section: Methodsmentioning

confidence: 99%

“…B lymphocytes/lymphoblasts were first enriched by immunomagnetic depletion of CD2 + CD14 + CD16 + WBC with goat anti‐mouse beads (Dynabeads ® , Invitrogen) . Then, CD19 + CD38 high (PB) and/or CD19 + CD38 low/neg (B cells) subsets were FACS‐sorted using a FACS Aria™ II cell sorter system (BD, Franklin Lakes, NJ, USA).…”

Section: Methodsmentioning

confidence: 99%

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Massive plasmablast response elicited in the acute phase of hantavirus pulmonary syndrome

et al. 2017

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Beside its key diagnostic value, the humoral immune response is thought to play a protective role in hantavirus pulmonary syndrome. However, little is known about the cell source of these antibodies during ongoing human infection. Herein we characterized B-cell subsets circulating in Andes-virus-infected patients. A notable potent plasmablast (PB) response that increased 100-fold over the baseline levels was observed around 1 week after the onset of symptoms. These PB present a CD3 CD19 CD20 CD38 CD27 CD138 IgA surface phenotype together with the presence of cytoplasmic functional immunoglobulins. They are large lymphocytes (lymphoblasts) morphologically coincident with the 'immunoblast-like' cells that have been previously described during blood cytology examinations of hantavirus-infected patients. Immunoreactivity analysis of white blood cell lysates suggests that some circulating PB are virus-specific but we also observed a significant increase of reactivity against virus-unrelated antigens, which suggests a possible bystander effect by polyclonal B-cell activation. The presence of this large and transient PB response raises the question as to whether these cells might have a protective or pathological role during the ongoing hantavirus pulmonary syndrome and suggest their practical application as a diagnostic/prognostic biomarker.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Massive plasmablast response elicited in the acute phase of hantavirus pulmonary syndrome

et al. 2017

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“…Although the signaling axis through this pathway is identical regardless of the type of infection, the outcome is substantially different, suggesting that the type I IFNs and the related IFN-inducible genes are able to create a favorable or unfavorable intracellular milieu to promote or disrupt the survival of invading pathogens [16]. Changes in mRNA levels of IFIT1, IFIT2, CXCR3 and CD163 have been validated by qPCR in previous studies [17][18][19]. Moreover, Kim et al [20] confirmed that five genes (IFN-γ, TNF-α, IL-2R, CXCL9, and CXCL10) could be used for the detection of Mtb infection, including active PTB disease and LTB with sensitivity of each gene above 80%.…”

Section: Discussionmentioning

confidence: 85%

Gene network in pulmonary tuberculosis based on bioinformatic analysis

Lyu

et al. 2020

Preprint

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Background: Pulmonary tuberculosis (PTB) is one of the serious infectious diseases worldwide; however, the gene network involved in the host response remain largely unclear. Methods: This study integrated two cohorts profile datasets GSE34608 and GSE83456 to elucidate the potential gene network and signaling pathways in PTB. Differentially expressed genes (DEGs) were obtained for Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis using Metascape database. Protein-Protein Interaction (PPI) network of DEGs was constructed by the online database the Search Tool for the Retrieval of Interacting Genes (STRING). Modules were identified by the plug-in APP Molecular Complex Detection (MCODE) in Cytoscape. GO and KEGG pathway of Module 1 were further analyzed by STRING. Hub genes were selected for further expression validation in dataset GSE19439. The gene expression level was also investigated in the dataset GSE31348 to display the change pattern during the PTB treatment. Results: Totally, 180 shared DEGs were identified from two datasets. Gene function and KEGG pathway enrichment revealed that DEGs mainly enriched in defense response to other organism, response to bacterium, myeloid leukocyte activation, cytokine production, etc. Seven modules were clustered based on PPI network. Module 1 contained 35 genes related to cytokine associated functions, among which 14 genes, including chemokine receptors, interferon-induced proteins and Toll-like receptors, were identified as hub genes. Expression levels of the hub genes were validated with a third dataset GSE19439. The signature of this core gene network showed significant response to Mycobacterium tuberculosis (Mtb) infection, and correlated with the gene network pattern during anti-PTB therapy. Conclusions: Our study unveils the coordination of causal genes during PTB infection, and provides a promising gene panel for PTB diagnosis. As major regulators of the host immune response to Mtb infection, the 14 hub genes are also potential molecular targets for developing PTB drugs.

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“…CXCR3 + B cells have previously been associated with migration to inflamed tissue [34][35][36][37] and may secrete interferon c (IFNc) 38 similar to their CD4 T cell counterpart; CXCR3 + Th1 cells. CXCR3 (CD183) ligands include the IFN-induced chemokines CXCL9 (MIG) and CXCL10 (IP-10), which have been shown to be systemically increased in high-risk populations.…”

Section: Discussionmentioning

confidence: 99%

Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial

Keefer

Zheng

Rosenberg

et al. 2016

AIDS Research and Human Retroviruses

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The success of an HIV vaccine will require induction of a protective immune response in the most at-risk populations. The increased incidence of HIV infection in high-risk populations is assumed to be primarily the result of more frequent exposure to the virus or a greater inoculum of the virus; however, underlying variations in immune homeostasis may also contribute to HIV susceptibility and potentially impact vaccine responses and those required for protection. As an effective humoral immune response is likely to be a critical component of a protective HIV vaccine, we evaluated the steady-state phenotypic profile of peripheral blood B cells by flow cytometry from participants in the HIV Vaccine Trials Network (HVTN) 203 Phase 2a HIV vaccine trial considered to be at higher risk and lower risk for HIV acquisition. Overall, high-risk participants exhibited increased frequency of unswitched IgM memory and activated switched IgDCD95 memory B cells than low-risk participants. Most (93%) of the high-risk male participants were men who have sex with men who engaged in high-risk sexual behavior. High-risk males had a significantly increased frequency of CXCR3 IgDCD95 B cells than low-risk males. These results suggest that high-risk populations have altered B cell homeostasis. The increased frequency of activated and memory B cells may suggest increased immune activation in high-risk populations, which may contribute to possible differential responses to HIV vaccine strategies.

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Human pleural B-cells regulate IFN-γ production by local T-cells and NK cells in aMycobacterium tuberculosis-induced delayed hypersensitivity reaction

Cited by 21 publications

References 40 publications

Massive plasmablast response elicited in the acute phase of hantavirus pulmonary syndrome

Massive plasmablast response elicited in the acute phase of hantavirus pulmonary syndrome

Gene network in pulmonary tuberculosis based on bioinformatic analysis

Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial

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