Human Platelet Acetylcholinesterase: The Effects of Anticholinesterases on Platelet Function

Abstract

SummaryPlatelet acetylcholinesterase (AChE) activity was measured in gel-filtered platelet preparations. Three different anticholinesteratic agents (eserine, neostigmine, and diiso- propylphosphorofluoridate) at final concentrations of 10 μM caused complete inhibition of AChE activity after 30 min incubation at room temperature with either platelet-rich plasma or gel-filtered platelets. Complete inhibition of platelet AChE had no effect on platelet aggregation, factor-3 availability, and plasma clot retraction. We conclude that platelet membrane AChE activity is not required for normal platelet function as measured by these in vitro parameters.

Amyloid Precursor Protein in Platelets of Patients With Alzheimer Disease

“…Although it is known that platelets express the repertoire of enzymes necessary for APP processing 35 and AChE, 36 it is difficult to ascribe the donepezil effect to a generic increase in the concentration of acetylcholine in biological fluids, which might in turn activate through its receptor(s) a biochemical cascade capable of affecting APP processing.…”