Human plasma fractionation and the impact of new technologies on the use and quality of plasma-derived products

Brodniewicz-Proba, Teresa

doi:10.1016/0268-960x(91)90016-6

Cited by 15 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Centrifugation separates blood components on the basis of their density gradients. Platelets are the lightest components, followed by WBCs, and RBCs are heaviest [26]. Therefore, RCF and time must be appropriate to obtain optimal separation of blood components.…”

Section: Discussionmentioning

confidence: 99%

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

2017

View full text Add to dashboard Cite

BackgroundPlatelet-rich plasma (PRP) has been expected for regenerative medicine because of its growth factors. However, there is considerable variability in the recovery and yield of platelets and the concentration of growth factors in PRP preparations. The aim of this study was to identify optimal relative centrifugal force and spin time for the preparation of PRP from canine blood using a double-centrifugation tube method.MethodsWhole blood samples were collected in citrate blood collection tubes from 12 healthy beagles. For the first centrifugation step, 10 different run conditions were compared to determine which condition produced optimal recovery of platelets. Once the optimal condition was identified, platelet-containing plasma prepared using that condition was subjected to a second centrifugation to pellet platelets. For the second centrifugation, 12 different run conditions were compared to identify the centrifugal force and spin time to produce maximal pellet recovery and concentration increase. Growth factor levels were estimated by using ELISA to measure platelet-derived growth factor-BB (PDGF-BB) concentrations in optimised CaCl2-activated platelet fractions.ResultsThe highest platelet recovery rate and yield were obtained by first centrifuging whole blood at 1000 g for 5 min and then centrifuging the recovered platelet-enriched plasma at 1500 g for 15 min. This protocol recovered 80% of platelets from whole blood and increased platelet concentration six-fold and produced the highest concentration of PDGF-BB in activated fractions.ConclusionsWe have described an optimised double-centrifugation tube method for the preparation of PRP from canine blood. This optimised method does not require particularly expensive equipment or high technical ability and can readily be carried out in a veterinary clinical setting.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1123-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

2017

View full text Add to dashboard Cite

show abstract

“…Blood plasma is a complex media, which contains a different type of proteins, enzymes, cofactors, inorganic ions, etc . Some of the blood plasma components can interact with quantum dots or tetrapyrrole compounds .…”

Section: Introductionmentioning

confidence: 99%

Investigation of Complexes of CdTe Quantum Dots with the AlOH-Sulphophthalocyanine Molecules in Aqueous Media

et al. 2013

View full text Add to dashboard Cite

The interaction between negatively charged CdTe quantum dots and AlOH-sulphophthalocyanine molecules in blood plasma and in aqueous solutions with different pH levels has been investigated. Similarly charged quantum dots and molecules are found to form complexes that exhibit an effective energy transfer. The efficiency of the energy transfer in these complexes is close to the theoretical FRET efficiency for a given donor−acceptor pair. It has been shown that in blood plasma, as well as in aqueous solutions, the formation of complexes of quantum dots with AlOH-sulphophthalocyanine molecules without using their charged groups allows preserving the photophysical properties of phthalocyanine molecules. A theoretical model of the formation of complexes between similarly charged quantum dots and molecules is proposed.

show abstract

“…Human plasma is a valuable raw material that produces functional proteins used in the therapy of coagulation, haematological and immunological disorders [ 1 , 2 , 3 , 4 ]. The quality of this starting material and the final products derived from it depends on two main factors: the blood or plasma of donors [ 5 ], and plasma preparation method [ 3 , 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and cellular markers differentiate recovered, in‐line filtered plasma, and plasma obtained by apheresis methods

et al. 2021

View full text Add to dashboard Cite

Background and Objectives: Assessment of plasma quality often focuses on the common safety tests for minimizing the risk of transmitting blood-borne pathogens.Little attention is paid to the possible quality attributes that ensure a consistent biochemical composition of plasma for fractionation. We therefore investigated the suitability of selected biochemical and haematological attributes that could be used as markers of plasma quality obtained by different separation and pre-treatment procedures.Material and Methods: We characterized six plasma types, including source plasma, plasma recovered by classic means and in-line filtered plasma, by determining the analytical attributes protein content, coagulation factors and markers of coagulation, contact and complement activation. Residual cell content and cell-specific variables were also measured. Results:We found relevant differences between the plasma types in complement activation, as indicated by C3a measurements, while thrombin antithrombin complex values and, to a minor extent, activated factor XII concentrations indicated only moderate differences in activation levels of coagulation and contact systems. The most striking differences, however, were detected in residual cell content and concentrations of the platelet-associated proteins, platelet factor 4 and β-thromboglobulin. We showed that leucocyte reduction filters disrupt cells. This includes platelets, thereby releasing the platelet-associated proteins platelet factor 4 and β-thromboglobulin, and leucocytes as demonstrated by the release of elastase from polymorphonuclear leucocytes. Furthermore, the filtration processing of whole blood can lead to activation of the complement system. Conclusion:Our results show that biochemical and cellular surrogate markers are valuable discriminators of plasma types.

show abstract

Human plasma fractionation and the impact of new technologies on the use and quality of plasma-derived products

Cited by 15 publications

References 35 publications

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

Investigation of Complexes of CdTe Quantum Dots with the AlOH-Sulphophthalocyanine Molecules in Aqueous Media

Biochemical and cellular markers differentiate recovered, in‐line filtered plasma, and plasma obtained by apheresis methods

Contact Info

Product

Resources

About