Human Placenta Is a Potent Hematopoietic Niche Containing Hematopoietic Stem and Progenitor Cells throughout Development

Robin, Catherine; Bollérot, Karine; Mendes, Sofia Silva; Haak, Esther; Crisan, Mihaela; Cerisoli, Francesco; Lauw, Ivoune; Kaimakis, Polynikis; Jorna, Ruud; Vermeulen, Mark; Kayser, Manfred; Linden, Reinier van der; Imanirad, Parisa; Verstegen, Monique M A; Nawaz-Yousaf, Humaira; Papazian, Natalie; Steegers, Eric; Cupedo, Tom; Dzierzak, Elaine

doi:10.1016/j.stem.2009.08.020

Cited by 188 publications

(171 citation statements)

References 51 publications

(74 reference statements)

Supporting

Mentioning

161

Contrasting

Unclassified

Order By: Relevance

“…Indeed, Zhang and colleagues have isolated mesenchymal progenitor cells from the human placenta that are capable of expanding long-term cultureinitiating cells from cord blood-derived CD34 + cells (Zhang et al 2004). We also have derived mesenchymal stromal cell lines from the human placenta (Robin et al 2009) and mouse placenta (I. Lauw, Master of Science thesis) from several stages of development. Some of these showed an extensive mesenchymal differentiation potential and also displayed a pericyte/perivascular cell phenotype similar to cells that have recently been reported to be the in vivo counterpart of mesesenchymal stromal/stem cells (Crisan et al 2008).…”

Section: Placenta Microenvironmentmentioning

confidence: 99%

The placenta as a haematopoietic organ

Ottersbach

Dzierzak

2010

Int. J. Dev. Biol.

Self Cite

View full text Add to dashboard Cite

The recent description of the placenta as a tissue rich in haematopoietic stem and progenitor cells has not only opened up a whole new line of investigation into how haematopoiesis is regulated in this unique mammalian tissue, but has also resulted in the revisiting of longstanding and yet unanswered questions about the significance of having multiple haematopoietic organs during development. Due to its remarkable capacity for haematopoietic stem/progenitor cell expansion, the study of placental haematopoiesis is also of obvious clinical interest. In the following pages, we summarise what is currently known about the haematopoietic regulatory processes in the murine placenta and describe our most recent data demonstrating that the human placenta, like its murine counterpart, is also a source of haematopoietic stem and progenitor cells throughout development.

show abstract

Section: Placenta Microenvironmentmentioning

confidence: 99%

The placenta as a haematopoietic organ

Ottersbach

Dzierzak

2010

Int. J. Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…All these approaches have suggested that discrete subsets of vascular ECs transiently exhibit blood-forming potential during vertebrate development. Recently a novel perspective to HSCs development came with the discovery that the human and murine placenta harbor a large pool of multipotential hematopoietic progenitors and stem cells, indicating that the placenta could play an important role in the establishment of hematopoiesis (Barcena et al, 2009;Robin et al, 2009;Mikkola et al, 2005; see also Ottersbach and Dzierzak; Mikkola, Dieterlen-Lièvre et al, in this issue). This process of HSCs emergence in the placenta is also intimately associated with large vessels (Rhodes et al, 2008) and therefore do highlight the role of vascular ECs in the generation of HSCs in vertebrates not only in the AGM, the umbilical and vitelline arteries but also in the placenta.…”

Section: Introductionmentioning

confidence: 98%

Definitive human and mouse hematopoiesis originates from the embryonic endothelium: a new class of HSCs based on VE-cadherin expression

Oberlin¹,

Hafny²,

Petit³

et al. 2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Hematopoietic stem cells (HSCs) arise first in the third week of human ontogeny inside yolk sac developing blood vessels, and independently, from the wall of the embryonic aorta and vitelline arteries one week later. HSCs produced in the yolk sac and in the embryonic truncal arteries migrate to and transiently colonize the embryonic liver (EL), and thereafter the bone marrow (BM), their permanent site of residence. At the moment, the origin of human HSCs is still controversial; one of the main hypotheses being that they are generated by hemogenic endothelial cells (ECs). To proove definitively the endothelial origin of HSCs that arise within the human embryo, we previously purified ECs from either the yolk sac or the truncal arteries and reported that they were able to produce blood cells in vitro. We then found that some of the HSCs present in the human EL were co-expressing vascular endothelial (VE)-cadherin, an endothelial marker, CD45, a pan-hematopoietic marker, and CD34, a common endothelial and hematopoietic marker, and demonstrated that these HSCs bearing a dual hemato-endothelial phenotype were endowed with remarkably high self renewal and proliferative potentials. Furthermore, a transgenic mouse model based on the VE-cadherin cis-regulating elements that we engineered to trace the fate of the first VE-cadherin expressing cells allowed us to clearly demonstrate that a majority of adult BM HSCs derived from a VE-cadherin ancestor. Altogether our studies strongly suggest that at least a part of both the human and the murine hematopoietic systems arise from an endotheliumlike ancestor.

show abstract

“…These hematopoiesis-potential mesoderm cells initiate primitive hematopoiesis after migrating to the yolk sac and can subsequently produce definitive hematopoietic cells [17,18]. The first definitive HSCs arise at the ventral wall of the dorsal aorta in the aorta-gonadmesonephros (AGM) region [19,20] and from murine and human placenta [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

TGFβ inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy

et al. 2011

View full text Add to dashboard Cite

Embryonic hematopoiesis is a complex process. Elucidating the mechanism regulating hematopoietic differentiation from pluripotent stem cells would allow us to establish a strategy to efficiently generate hematopoietic cells. However, the mechanism governing the generation of hematopoietic progenitors from human embryonic stem cells (hESCs) remains unknown. Here, on the basis of the emergence of CD43 + hematopoietic cells from hemogenic endothelial (HE) cells, we demonstrated that VEGF was essential and sufficient, and that bFGF was synergistic with VEGF to specify the HE cells and the subsequent transition into CD43 + hematopoietic cells. Significantly, we identified TGFβ as a novel signal to regulate hematopoietic development, as the TGFβ inhibitor SB 431542 significantly promoted the transition from HE cells into CD43 + hematopoietic progenitor cells (HPCs) during hESC differentiation. By defining these critical signaling factors during hematopoietic differentiation, we can efficiently generate HPCs from hESCs. Our strategy could offer an in vitro model to study early human hematopoietic development.

show abstract

Human Placenta Is a Potent Hematopoietic Niche Containing Hematopoietic Stem and Progenitor Cells throughout Development

Cited by 188 publications

References 51 publications

The placenta as a haematopoietic organ

The placenta as a haematopoietic organ

Definitive human and mouse hematopoiesis originates from the embryonic endothelium: a new class of HSCs based on VE-cadherin expression

TGFβ inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy

Contact Info

Product

Resources

About