Human Periosteal Derived Stem Cell Potential: The Impact of age

Abstract

There is a great deal of interest in the understanding of possible age-related changes in Mesenchymal Stem Cells in view of their use for regenerative medicine applications. Given to the outmost standing of periosteum in bone biology and to probe data for a cell-based therapy promoting graft osseointegration in the elderly, we tried to identify specific aging markers or pattern of expression in human periosteal precursor cells. Immunohistochemical detection of Ki67 and p53, Nitric Oxide production and qRT- PCR of a selected gene panel for osteoblastic differentiation, bone remodeling and stemness were evaluated. We confirmed that both Ki67 and p53 are noteworthy indicators of senescence in human periosteal precursor cells and their expression significantly correlate with cell NO production. Moreover, cell age affects genes involved in bone remodeling, with a significant increase in interleukin-6 mRNA expression and receptor activator of nuclear factor kappa-B ligand/osteoprotegerin ratio. The analysis of mRNAs of genes involved in pluripotency regulation and self-renewal of stem cells, evidenced changes at least in part related to bone remodeling. We believe that this is the first study taking on age-related changes in human periosteal precursor cells, and paving the way toward new regenerative medicine strategies in bone aging and/or bone metabolic diseases.

Displacement in root apex and changes in incisor inclination affect alveolar bone remodeling in adult bimaxillary protrusion patients: a retrospective study

“…Such equation may customize both periodontal and orthodontic consideration in adult patients, especially determining whether bone augmentation surgery was needed to aid orthodontic therapy, commonly recognized as surgically facilitated orthodontic therapy [SFOT] [32] or periodontally accelerated osteogenic orthodontics [PAOO]) [33] . Our present equation further supports the conception that bodily retraction would lead to proximity of root apex to palatal cortical plate and extensive alveolar bone resorption [34] ,and this jeopardy is particularly pertinent in adults as numbers and regeneration capability of osteoblasts in the periosteum reduces with aging [35] .…”

Biofabrication and Bone Tissue Regeneration: Cell Source, Approaches, and Challenges

“…Despite these advances, there are still few comparable data in the literature on long-term in vitro and in vivo characterization of bioprinted and biofabricated bone constructs. The improve accuracy of in silico predictive models would also reduce the numbers of animals used in in vivo studies (Fedorovich et al, 2011; Ferretti et al, 2015; Vozzi et al, 2016). …”

Treatment of intrabony defects with modified perforated membranes in aggressive periodontitis: a 12-month randomized controlled trial

“…The inclusion criteria were as follows: (1) no systematic medical compromising conditions; (2) no medications affecting periodontal status; (3) only light smokers were included (< 10 cigarettes/day); (4) not pregnant or lactating; (5) familial aggregation (history of periodontitis in parents or siblings); (6) presence of at least two teeth with probing pocket depth (PPD) ≥ 6 mm and CAL ≥ 5 mm associated with an intrabony defect of at least 3 mm as detected in diagnostic periapical radiographs; (7) full mouth plaque score (FMPS) ≤ 20%; (8) bleeding on probing score (BoP) ≤ 20%; (9) teeth had to be vital or properly treated; (10) no furcation involvement of the teeth presenting the intraosseous defects; (11) the width of keratinized tissue on the facial aspect of the selected teeth ≥ 2 mm.…”

“…The high osteogenic potential of jaw periosteal cells, especially MSCA-1 + fraction, is closely related to the expression of lipoprotein receptor-related protein 6 (LRP-6), stanniocalcin 1 (STC-1), and tissue inhibitor of metalloproteinases-4 (TIMP-4) [5]. Periosteal cell populations modify their potential with age, with a significant increase in IL-6 mRNA expression and receptor activator of nuclear factor kappa-B ligand/osteoprotegerin ratio [6]. In vivo studies indicated that PDPCs are indispensable for bone graft healing and remodeling [7].…”